Proteomic Analysis and Comparison of Protein in Tibetan pork and Ordinary pork using TMT-coupled LC-MS/MS

The objective of this study was to comparatively analyze protein of Tibetan pork and ordinary pork using liquid chromatography tandem-mass spectrometry (LC − MS/MS) combined with tandem mass tag (TMT) labeling. The results showed that 2447 proteins were identified and 249 proteins were significantly changed including 165 significantly up-regulated and 84 significantly down-regulated proteins. The more abundant proteins of Tibetan pork included NADH dehydrogenase, Pyruvate dehydrogenase E1, Very long-chain specific acyl-CoA dehydrogenase, ATPase inhibitor and Cytochrome b-c1 complex subunit 7, which were up-regulated. A Gene Ontology (GO) analysis showed that these proteins mainly participated in biological process, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and the Clusters of Orthologous Groups of proteins (COG) analysis showed that they may be related to energy metabolism and conversion pathway of metabolism process. According to the protein-protein interaction (PPI) analysis, these proteins interacted significantly. This study revealed the Tibetan pork’s advantages in the aspects of energy metabolism compared with ordinary pork at the protein levels.