Determination of Prostate-Specific Antigen Via The Assembly of a Two-Dimensional Nanoplatform

Abstract Two-dimensional platforms with favorable features are highly expected for diverse application. In this work, we report a highly sensitive and selective “turn-on” fluorescent nanoprobe for prostate-specific antigen (PSA) detection base on cobalt oxyhydroxide nanosheets (CoOOH NSs). CoOOH NSs are employed as the suitable sensing hosts, in which fluorescein amidite-(abbreviated as FAM) labeled aptamer probe (PA) has been adsorbed on nanosheets. Energy transfer between substrate and optical species has switched off the fluorescence of PA. The strong affinity of PA to the target PSA induces the formation of a rigid aptamer structure and the integration with the CoOOH NSs has been drastically affected. The recognition process has been followed by the release of the aptamer probe PA from the nanosheet surface and the green luminescence has been recovered. The dynamic nano-sensor exhibits highly sensitive and accurate analytical performance toward PSA with a linear detection range from 0.1 to 5 nM and a detection limit of 56.1 pM. Therefore, a simple and efficient sensing platform for the detection of prostate cancer can be established.