Delivery of Moringa oleifera Extract via PLA-PEG-FA/Chitosan-PLA NPs into Breast Cancer Cell Lines

Delivery of Moringa oleifera Extract via PLA-PEG-FA/Chitosan-PLA NPs into Breast Cancer Cell Lines | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Article Delivery of Moringa oleifera Extract via PLA-PEG-FA/Chitosan-PLA NPs into Breast Cancer Cell Lines Mehran Noruzpour, Rasool Asghari Zakaria, Nasser Zare, Shima Bourang, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4516162/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Nanotechnology offers a promising solution for overcoming chemotherapy challenges in cancer treatment by enabling targeted drug delivery. Moringa oleifera Lam. containing significant medicinal compounds, is known for its anticancer and antioxidant characteristics. In this study, we investigated the effectiveness of polymeric PLA-PEG-FA/Chitosan-PLA nanoparticles on targeted delivery of this plant’s extract and their effects on the growth and viability of cancer cell lines. Aqueous extracts were obtained from plant and callus tissues of M. oleifera. A solvent diffusion technique was employed to prepare PLA-PEG-FA/PLA-Chitosan nanoparticles. The copolymers were encapsulated with moringa aqueous extract, its callus extract, and pure quercetin. The physicochemical characteristics of nanoparticles were evaluated. Finally, drug encapsulation efficiency, drug release, in vitro cytotoxicity, and cell apoptosis analysis were conducted to study the drug delivery efficiency to MCF-7 and BT-549 breast cancer cell lines. PLA-PEG-FA/PLA-Chitosan nanoparticles synthesized exhibit a spherical shape with an average size of 230 nm and a zeta potential of -6.4 mV. The spectrophotometric assessment revealed that drug release was more pronounced at acidic pH. The water-soluble tetrazolium salt (WST-1) assay demonstrated the biocompatibility of the drug nanocarriers and their low toxicity. Furthermore, the highest percentage of pre- and post-apoptotic cells was detected in the BT-549 cell line treated with PLA-PEG-FA/Chitosan-PLA/Quercetin nanoparticles (31.2 and 7.15%, respectively). it was concluded that PLA-Chitosan and PLA-PEG-FA/Chitosan-PLA copolymers nanoparticles exhibit a high capability for the targeted delivery of Quercetin, the anticancer compounds present in the Moringa water extract (from plant and callus tissue) towards cancer cells. Biological sciences/Biotechnology Health sciences/Molecular medicine Physical sciences/Chemistry/Medicinal chemistry Aqueous Extract Copolymer nanoparticle Moringa Oliefera Quercetin Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4516162","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":316487128,"identity":"6c4decf1-d083-4127-9bc4-375d756772f8","order_by":0,"name":"Mehran Noruzpour","email":"","orcid":"","institution":"University of Mohaghegh Ardabili","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mehran","middleName":"","lastName":"Noruzpour","suffix":""},{"id":316487129,"identity":"5a69253d-0c8f-4ac7-bd19-15d921a291fd","order_by":1,"name":"Rasool Asghari 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Moringa oleifera Lam. containing significant medicinal compounds, is known for its anticancer and antioxidant characteristics. In this study, we investigated the effectiveness of polymeric PLA-PEG-FA/Chitosan-PLA nanoparticles on targeted delivery of this plant’s extract and their effects on the growth and viability of cancer cell lines. Aqueous extracts were obtained from plant and callus tissues of M. oleifera. A solvent diffusion technique was employed to prepare PLA-PEG-FA/PLA-Chitosan nanoparticles. The copolymers were encapsulated with moringa aqueous extract, its callus extract, and pure quercetin. The physicochemical characteristics of nanoparticles were evaluated. Finally, drug encapsulation efficiency, drug release, in vitro cytotoxicity, and cell apoptosis analysis were conducted to study the drug delivery efficiency to MCF-7 and BT-549 breast cancer cell lines. PLA-PEG-FA/PLA-Chitosan nanoparticles synthesized exhibit a spherical shape with an average size of 230 nm and a zeta potential of -6.4 mV. The spectrophotometric assessment revealed that drug release was more pronounced at acidic pH. The water-soluble tetrazolium salt (WST-1) assay demonstrated the biocompatibility of the drug nanocarriers and their low toxicity. 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