Detection of astrocyte epigenetic memory in in vitro systems, experimental autoimmune encephalomyelitis and multiple sclerosis samples

The paper investigates whether previously described astrocyte pro-inflammatory “epigenetic memory” is genuine or instead caused by immune-cell contamination, after bioinformatic arguments by others suggested myeloid contamination. The authors report high astrocyte purity in both in vivo and in vitro systems, reproduce memory responses across five independent pure astrocyte in vitro models, and show dependence on the histone acetyltransferase p300. They further reanalyze five independent single-cell RNA-seq datasets from experimental autoimmune encephalomyelitis and multiple sclerosis using the Liddelow lab bioinformatic pipeline with specific purity and cell-quality criteria, detecting astrocyte epigenetic memory. The paper’s main caveat is that it relies on bioinformatic purity/cell-quality filtering and its ability to rule out contamination as the explanation. Relevance to endometriosis: the paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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