Coagulation activity of Chitin Binding Protein to the Turbid Pond Water from Garden Candytuft Seeds

Abstract In advanced nations, reservoir water is frequently utilized for consumption or domestic use, and its turbidity is higher during wet seasons. It requires many artificial coagulants, which increases the cost of therapy. Finding natural alternatives, particularly those derived from plants, has become more relevant because of the negative health and environmental implications of traditional synthetic substances used in water clarification. In this study, a natural coagulant protein called chitin binding protein (CBP) purified from the seeds of Iberis umbellata L. was utilized to treat water and remove turbidity. To increase the effectiveness of turbidity removal, this study focuses on the extraction, isolation, and purification of natural coagulants from the seeds of I. umbellata L. At pH 7, the maximum turbidity reduction effectiveness was attained. The molecular weight of CBP was approximately 14 kDa, as revealed by SDS-PAGE and purified by spin column chromatography. The CBP of I. umbellata showed coagulation activity against synthetic clay solution and pond water. The coagulation results showed that CBP was 87% and Moringra extract showed 89% activity against the synthetic clay solution after 120 min. CBP from I. umbellata showed 83% and Mo extract 82% coagulation activity against the pond water. CBP from I. umbellata may be a viable natural coagulant for water treatment based on its coagulation performance against severely murky pond water.