Exon 9LEPRGene SNP Polymorphism of Hybrid Chickens F2KambroCrossbreeds of ♀ F1Kambrowith ♂ F1Kambro

The implementation of the T-ARMS PCR method in the detection of single nucleotide polymorphisms (SNPs) in the LEPR gene in chicken DNA samples has never been conducted. This research aims to design a specific protocol for exon 9 LEPR gene SNPs detection and detect LEPR gene expression or LEPR SNPs in Pelung chicken samples, F 1 Pelung , Layer, Broiler Cobb 500, F 1 Kambro chicken and F 2 Kambro chicken using the T-ARMS PCR method. Determination of LEPR gene correlation degree on Body Weight (BT) and Egg Productivity (PT) in F 1 Kambro population and F 2 Kambro . Qualitative phenotype parameters showed six groups of segregated phenotypes compared to F 1 Kambro chicken. Growth of F 2 Kambro chicken weight reached 753.36 ± 155.31 grams in 8 weeks was not significant for F 1 Kambro chicken due to inbreeding depression (Fx = 25%, IR = 4.925%) and transversion of A LEPR allele mutations. Specific protocol detection of exon 9 LEPR gene SNPs using the T-ARMS PCR method can detect C127A LEPR mutations with IP: OP ratio 10:1 pmol / µM, chicken DNA template concentration of 100 ng / µL with annealing temperature of 55.7° C / 30s. The transversion mutation of C127A of LEPR exon 9 SNP were detected in DNA samples of F 1 Kambro hens (80%), F 2 Kambro roosters (20%), Broiler Cobb 500 hens (75%). The mutations were not detected in Layer, Pelung Blirik Hitam chicken and F 1 Pelung populations.