Hylocereus Polyrhizus Peel Ethanol Extract- the Potential Effect to Tumor Necrosis Factor-Α, Macrophage, and Matrix Metalloproteinase-9 in Endometriosis Mice

On endometriosis, macrophage and TNF-α was found in higher concentration. Tumor Necrosis Factor-α activates NF-κB pathway and increase the expression of MMP-9’s gen. NF-κB pathway can be blocked by Hylocereus polyrhizus peel. The objective of this study was to know the effect of Hylocereus polyrhizus peel ethanol extract at dose 0,25; 0,5 mg/gram BW/day; and 1 mg/gram on TNF-α concentration, number of macrophage, and MMP-9 expression on mouse model of endometriosis. This study was a laboratory experimental research. Thirty female mice were used as samples and divided into 5 groups: 1 positive control, 1 negative control, and 3 treatment groups. Positive control and treatment groups were induced as model of endometriosis for 14 days. The next 14 days, Na-CMC 0,5% was given to both control groups, while Hylocereus polyrhizus peel ethanol extract dose 0,25; 0,5 mg/gram BW/day; and 1 mg/gram were given to treatment groups orally. Peritoneum fluid and endometriosis lesion were examined. There were significantly differences on TNF-α concentration (p=0.021), number of macrophage (p=0.00), and MMP-9 expression (p=0.002) among control groups and treatment groups. TNF-α concentrations were higher on treatment groups. Hylocereus polyrhizus peel ethanol extract can be used as alternative therapy for attenuating the alteration of macrophage and MMP-9 in endometriosis disease