Wound healing potential: Evaluation of molecular profiling and amplification of Lucilia sericata angiopoietin-1 mRNA mid-part

Abstract Objective: High prevalence of chronic ulcers and burden of disease necessitate the increasingly significant production of new recombinant proteins in the world. The angiopoietin-1 enzyme is a part of growth factors group which is secreted by Lucilia sericata (Diptera: Calliphoridae) larvae when they are exposed to wounds to ensure maggot therapy. It is one of the most potent proteins in wound healing. Given its essential role, angiopoietin-1 gene of L. sericata was characterized which provided some basic information on its identity. Results: The mid-part of angiopoietin-1 mRNA sequence was thus characterized based on the design of disparate primers such as exon-exon junction, conserved regions, and specific region primers via conventional polymerase chain reaction (PCR). Its structural features was configured by in silico method. The sequence of mid-part (390 bp) of angiopoietin-1 was determined empirically, and BLAST analysis unravelled its high identity (85%) with the sequence of angiopoietin-1 mRNA of the larval housefly, Musca domestica . The homology of this enzyme also exhibited that its nucleic acid sequence was very similar to the domains of angiopoietin-1 in Lucilia cuprina . The current data are critical to evaluate the action of this enzyme in recombinant protein production in future molecular studies on wound healing.