The effects of mifepristone intervention on Ki-67 mRNA and Caspase-3 mRNA expressions in cultured glandular cells from entopic endometrium in adenomyosis

Objective To explore effects of mifepristone intervention on proliferation and apoptosis of cultured glandular cells from entopic endometrium in adenomyosis.Methods The expressions of Caspase-3 mRNA and Ki-67 mRNA in these cells were detected with semi-quantitative RT-PCR.Results Compared with control group,higher level of Ki-67 mRNA expression in the glandular cells was found in adenomyosis group than in control group during both proliferative and secretory phases(P0.05).The expression of Ki-67 mRNA was decreased after intervention with mifepristone(P0.05).During proliferative phase,the expression of Caspase-3 mRNA in the glandular epithelium cells of adenomyosis group was weaker than that in control group(P0.05),but no difference was observed during the secretory phase(P0.05).In proliferation phase,the expression of Caspase-3 mRNA in mifepristone group was stronger than that in adenomyosis group(P0.05),but again,the two groups did not differ during the secretory phase.Conclusion Disturbance of apoptosis and proliferation in glandular epithelium cell from endometrium in adenomyosis may be involved in the pathogenesis of adenomyosis.Mifepristone was shown to induce apoptosis and inhibit proliferation of glandular epithelium cells from endometrium,hence providing control of adenomyosis.