CD4+, CD8+ and CD4+CD25+ T lymphocytes in peripheral blood and peritoneal fluid of women with endometriosis - preliminary report

Jarosław Górski, Krzysztof Szyłło, Małgorzata Banasik, Przemysław Lewkowicz, H Tchórzewski
In: Archives of Medical Science, Vol 3, Iss 1, Pp 37-42 (2007) · 2007 · W4391505141
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This study found higher CD4+CD25+ T cells in peripheral blood but not peritoneal fluid of women with endometriosis, along with decreased IL-10, suggesting altered T cell populations may contribute to disease development.

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This preliminary study examined CD4+, CD8+, and CD4+CD25+ T lymphocyte subpopulations in the peripheral blood and peritoneal fluid of 24 women with laparoscopically diagnosed endometriosis, compared with 12 women without endometriosis, using flow cytometry. The authors found a higher percentage of CD4+CD25+ cells in peripheral blood of women with endometriosis, while no between-group difference was observed for peritoneal fluid, and they report that changes in CD4+CD25+ affected the CD8+ compartment. Peripheral blood lymphocytes from women with endometriosis produced less IL-10 at baseline and showed a lower IL-10 increase after PHA stimulation than controls. The paper is explicitly preliminary, and the main limitation stated is the small sample size. This paper is centrally about endometriosis—measuring T lymphocyte subsets and IL-10 in peripheral blood and peritoneal fluid to characterize immune differences in endometriosis.

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Abstract

Introduction: CD4+CD25+ cells play a major role in maintenance of immunological self-tolerance and negative control of pathological and physiological immunological reactions. Their absence or ill function leads to autoimmunization, immunopathology and allergy. The aim of this study was to investigate the prevalence of T lymphocyte subpopulations in peripheral blood and peritoneal fluid of women with endometriosis, as well as IL-10 generation by peripheral blood lymphocytes. Material and methods: The studied group comprised 24 women with endometriosis diagnosed during laparoscopy. The studied group was divided into two parts, depending on progression stage. The control group comprised 12 women without endometriosis. The percentages of subpopulations of T lymphocytes were evaluated via flow cytometry with IMK Plus test (BD Biosciences, USA). IL-10 concentration was analyzed by enzyme-linked immunosorbent assay – ELISA (R&D System, USA). Results: In the present study we observed higher percentage of CD4+CD25+ cells in peripheral blood of affected women in comparison with healthy subjects. We did not observe such a difference for the peritoneal fluid between the groups. Changes in number of CD4+CD25+ affect CD8+ shift. We observed a decreased amount of IL-10 in peripheral blood in women with endometriosis. The increase after PHA (phytohaemagglutinin) stimulation was lower in women suffering from endometriosis than in the control group. Conclusions: The quantitative disproportion of CD4+CD25+ T cells between peripheral blood and peritoneal fluid can affect the onset and development of endometriosis. The impaired migration and function of Treg cells alter composition of peritoneal fluid, which favours the onset and development of endometriosis.
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Archives of Medical Science (Mar 2007) CD4+, CD8+ and CD4+CD25+ T lymphocytes in peripheral blood and peritoneal fluid of women with endometriosis - preliminary report Abstract Introduction: CD4+CD25+ cells play a major role in maintenance of immunological self-tolerance and negative control of pathological and physiological immunological reactions. Their absence or ill function leads to autoimmunization, immunopathology and allergy. The aim of this study was to investigate the prevalence of T lymphocyte subpopulations in peripheral blood and peritoneal fluid of women with endometriosis, as well as IL-10 generation by peripheral blood lymphocytes. Material and methods: The studied group comprised 24 women with endometriosis diagnosed during laparoscopy. The studied group was divided into two parts, depending on progression stage. The control group comprised 12 women without endometriosis. The percentages of subpopulations of T lymphocytes were evaluated via flow cytometry with IMK Plus test (BD Biosciences, USA). IL-10 concentration was analyzed by enzyme-linked immunosorbent assay – ELISA (R&D System, USA). Results: In the present study we observed higher percentage of CD4+CD25+ cells in peripheral blood of affected women in comparison with healthy subjects. We did not observe such a difference for the peritoneal fluid between the groups. Changes in number of CD4+CD25+ affect CD8+ shift. We observed a decreased amount of IL-10 in peripheral blood in women with endometriosis. The increase after PHA (phytohaemagglutinin) stimulation was lower in women suffering from endometriosis than in the control group. Conclusions: The quantitative disproportion of CD4+CD25+ T cells between peripheral blood and peritoneal fluid can affect the onset and development of endometriosis. The impaired migration and function of Treg cells alter composition of peritoneal fluid, which favours the onset and development of endometriosis.

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endometriosis

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