Folic acid-Chitosan Coated Stylosin Nanostructured Lipid Carriers: Fabrication, In Vitro-In Vivo Assessment in Breast Malignant Cells

Abstract Objective: Synthesis of Targeted Nanostructure Lipid Carriers for stylosin (STY-CFN-NPs) delivery to MCF-7 CellsMaterials and Methods: STY-CFN-NPs were formulated via the homogenization and ultra-sonication technique. After evaluating the amount of drug encapsulation and FA binding, toxicity effect of the STY and STY-CFN-NPs on MCF-7 cells was measured by MTT method. Cell cycle analysis, AO/PI staining and qPCR to assess the inducing of apoptosis as well as Tubo cancer cell inoculated mouse model for antitumor properties of STY-CFN-NPs were used.Results: Significant increases in nanoparticle size and changes in zeta potential were observed after FA-CS coating on nanoparticles. Slow release of the STY within 144 h as well as acceptable rate for STY encapsulation efficiency (92.4% and FA binding (52.5%) to the STY-CFN-NPs (PS: 66.26±3.02 nm, ZP: 29.54±1.01 mV and PDI: 0.32±0.01) was reported. STY-CFN-NPs exhibited higher toxicity compared to STY suspension and treatment with STY-CFN-NPs was lead to the increased apoptotic cells, stopped cells in SubG1 phase and also increased Caspase and Bax expression and decreased BCL-2 and BCL-XL expression in In vitro and decreased the size of murine tumors (54.57% in 16 days) in In vivo.Conclusion: The results showed STY-CFN-NPs has good potential for breast cancer management