A novel hexasegmented virus isolated from the phytopathogenic fungus Verticillium nonalfalfae

A novel hexasegmented virus isolated from the phytopathogenic fungus Verticillium nonalfalfae | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article A novel hexasegmented virus isolated from the phytopathogenic fungus Verticillium nonalfalfae Vanja Miljanić, Sead Sabanadzovic, Nina Aboughanem-Sabanadzovic, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9475253/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 9 You are reading this latest preprint version Abstract In this study, we describe a novel virus, tentatively named Verticillium nonalfalfae virus M (VnaVM), identified in the phytopathogenic fungus Verticillium nonalfalfae isolated from hop ( Humulus lupulus L.). The VnaVM genome consists of six double-stranded RNA (dsRNA) segments ranging from 1051 to 2401 bp. DsRNAs 1–3 encode an RNA-dependent RNA polymerase (RdRp), a hypothetical protein and a methyltransferase (MTR), respectively, while dsRNA6 code for a proline-alanine-serine-rich protein (PASrp). These four proteins share less than 58% amino acid identity with homologs encoded by viruses in the family Polymycoviridae . Products encoded by dsRNAs 4 and 5 show no similarity to known proteins, and their roles in the viral life cycle are unknown. Phylogenetic analysis of RdRp amino acid sequences, along with other results, support classifying VnaVM as a representative of a novel species within the genus Multimycovirus (family Polymycoviridae ). To our knowledge, this is the first report of a polymycovirid infection in a member of the genus Verticillium . Figures Figure 1 Figure 2 Introduction Polymycovirids, viruses belonging to the Polymycoviridae family, are a group of recently described mycoviruses. The first polymycovirid was discovered in the human pathogenic fungus Aspergillus fumigatus [ 1 ]. Since then, polymycoviruses have also been identified in a range of plant- [ 2 – 6 ] and insect-associated fungi [ 7 – 10 ]. These viruses are notable for their unusual genomic structure, which typically comprises four to eight dsRNA segments, each containing one, or rarely two, putative open reading frames (ORFs) flanked by 5′- and 3′-untranslated regions (UTRs). All known polymycovirids share a “core” of four conserved genomic segments coding for RNA-dependent RNA polymerase (RdRp), a hypothetical cysteine-rich protein, putative methyltransferase (MTR), and a proline-alanine-serine-rich protein (PASrp) [ 11 ]. The family Polymycoviridae , officially recognized in 2020 as a monogeneric, has recently been reorganized and expanded to include 28 species classified into three genera: Polymycovirus , Plurimycovirus and Multimycovirus [ 12 , 13 ]. Evolutionarily, polymycovirids are distantly related to hadakavirids (family Hadakaviridae ), both currently classified in the order Xenadelphovirales , class Mycopleornaviricetes [ 12 , 13 ]. Some polymycovirids have been shown to alter various host features, such as growth, pigmentation, sporulation, changes in virulence, fungicide susceptibility, and metabolic pathways [ 7 , 14 – 16 ]. Verticillium wilt, caused by ascomycete filamentous fungi in the genus Verticillium , is among the most devastating plant vascular fungal diseases worldwide. Ten species are currently recognized in the genus Verticillium sensu stricto ( V. albo-atrum , V. alfalfae , V. dahliae , V. isaacii , V. klebahnii , V. longisporum , V. nonalfalfae , V. nubilum , V. tricorpus , and V. zaregamsianum ) [ 17 ]. These soilborne fungi can persist in soil for several years without a host by producing melanized resting structures [ 18 ]. Following host-induced germination of these resting structures, the fungus penetrates the root system and subsequently colonizes the xylem, resulting in wilting and, in severe cases, complete dieback [ 19 ]. Hop ( Humulus lupulus L.) is one of the most susceptible hosts to V. nonalfalfae . Highly virulent or lethal pathotypes spread rapidly throughout hop plantations, resulting in significant yield losses and long-term impacts on hop production [ 20 ]. To date, a total of seven mycoviruses have been reported from Verticillium spp.: Verticillium dahliae ormycovirus 1 (VdOMV1) and VdOMV2 (family Ormycoviridae ), Verticillium dahliae chrysovirus 1 (VdCV1; family Chrysoviridae ), Verticillium dahliae partitivirus 1 (VdPV1; family Partitiviridae ), Verticillium dahliae magoulivirus 1 (VdMoV1; family Botourmiaviridae ), Verticillium dahliae RNA virus 1 (VdRV1; unassigned non-segmented + RNA virus), and Verticillium albo-atrum partitivirus 1 (VaaPV1; family Partitiviridae ) [ 21 – 26 ]. In this study, we report the molecular characterization of the novel hexasegmented virus from the phytopathogenic fungus V. nonalfalfae isolate 274VD, tentatively named Verticillium nonalfalfae virus M (VnaVM). Provenance of the virus material The source material for dsRNA isolation was V. nonalfalfae isolate 274VD, recovered from an infected hop plant in Slovenia in 2024. Fungal mycelia were harvested from a pure culture grown at room temperature on a cellophane membrane overlaid on the surface of ½ Czapek Dox plate (supplemented with 1g/l of malt extract, 1 g/l of yeast extract, 1g/l of peptone, and 15 g/l of agar). DsRNA was extracted from fungal mycelium using cellulose C6288 (Sigma-Aldrich, St. Louis, MO, USA), following the method described by Mokhtari and Ali [ 27 ] with minor modifications. The nature of the dsRNA was confirmed by digestion of the extracts with DNase I (New England BioLabs) and S1 nuclease (Thermo Fisher Scientific). The purified dsRNAs were analyzed in a 1% (wt/vol) agarose gel and visualized under UV light after staining with 0.1 µg/mL ethidium bromide. Six dsRNA segments ranging from 1 to 2.4 kb were observed. The library was constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) according to the manufacturer’s instructions. The yield and size distribution of the amplified cDNA library were determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). Sequencing was performed on the Ion GeneStudio S5 Prime System (Thermo Fisher Scientific). Adapter sequences were removed from raw reads before processing with bioinformatics tools CLC Genomic Workbench and Genomics Server (Qiagen). The Basic Local Alignment Search Tool (BLAST) of the National Center for Biotechnology Information (NCBI) was used for sequence comparisons. Potential open reading frames (ORFs) and putative conserved domains were predicted using ORF Finder and the Conserved Domain Database (CDD) of NCBI, respectively. Proteins with no matches in CDD, were further investigated using HHPred for remote homology and structure prediction via pairwise comparison of profile hidden Markov models (HMMs) [ 28 ]. Phylogenetic analysis was conducted with the IQ-Tree v.3.0.1 [ 29 ] on amino acid sequences of RdRp aligned with MAFFT v.7 [ 30 ] under the best-fit model Q.PFAM + F+I+G4 identified by ModelFinder [ 31 ]. The tree was visualized with iTOL v7 [ 32 ]. The complete sequences of dsRNA1-6 were obtained by rapid amplification of cDNA ends (RACE) using the SMART™ RACE cDNA Amplification Kit (Takara). All amplified fragments were purified, cloned, and Sanger sequenced. The complete sequence of each dsRNA segment was determined by sequencing at least three independent clones. Primer sets used in this study are detailed in Supplementary Table 1. The six dsRNAs are deposited in the GenBank database under the accession numbers PX960521-PX960526. Sequence properties A total of six dsRNA segments were extracted from V. nonalfalfae isolate 274VD (Fig. 1A) and visualized by agarose gel electrophoresis (Fig. 1B). Each dsRNA segment contained a single open reading frame on the coding strand flanked by UTRs (Fig. 1C). The 5′-UTRs of the coding strands of dsRNAs 1–6 range from 30 to 117 nt in length and share a highly conserved 30-nt sequence (ACAUGGGGGAACAAAAYHWUAUAWMYKYRC) (Supplementary Fig. 1A). The 3′-UTRs vary from 64 to 299 nt in length and lack extensive conserved elements. Secondary structure prediction revealed that the 5′ and 3′ terminal regions of all six dsRNAs fold into stem–loop structures, as exemplified by dsRNA1 (Supplementary Fig. 1B). VnaVM dsRNA1 contains one large ORF1 (nt 31-2337), which encodes a protein of 768 amino acids (aa) with an estimated molecular mass ( Mr ) of 84.07 kDa. The 84K protein shares high amino acid identity with RdRps encoded by members of the family Polymycoviridae , particularly with that encoded by Exserohilum turcicum polymycovirus 1 (XBY85583.1) and Setosphaeria turcica polymycovirus 1 (UMZ55610.1) (57.96%, query cover = 100%, e-value = 0.0). VnaVM RdRp contains the six conserved motifs, including the characteristic tetrapeptide GDNQ in motif VI (Fig. 2A). This tetrapeptide is also found in members of the mycoviral families Polymycoviridae and Hadakaviridae , as well as in some (-)RNA viruses belonging to the order Mononegavirales [ 33 ]. VnaVM dsRNA2 contains a single ORF (ORF2, nt 79-2172) that encodes a hypothetical protein of 697 aa ( Mr 74.03 kDa) of unknown function, which shares conserved aa content (25–48%) with the corresponding proteins of polymycovirids and Hadaka virus 1. The third dsRNA of VnaVM is also monocistronic, with a coding region spanning nucleotides 47 to 1900 and encoding a protein of 617 aa ( Mr 66.56 kDa). The ORF3-encoded protein shows the highest identity of ~ 48% with homologs encoded by dsRNA3 of Alternaria alternata polymycovirus 1 (QVK45098.1) and Plasmopara viticola lesion associated polymycovirus 1 (QHG11068.1) (query cover = 89%; e-value = 7e-149). CDD-based searches indicate that the ORF3 protein contains a conserved RsmD superfamily domain from amino acid positions 385 to 621 (COG0742; e-value = 1.15e-06), suggesting its enzymatic nature as a SAM-dependent methyltransferase. The sixth dsRNA contains a single ORF (ORF6, nt 118–906) encoding a 262-aa product ( Mr 28.03 kDa) relatively rich in proline (8.02%), alanine (10.68%), and serine (8.02%). A BLASTp search showed that the ORF6 protein sequence shares 56.70% identity with PASrps of Beauveria bassiana polymycovirus 4 (QRF54816.1), Beauveria bassiana polymycovirus 4 − 2 (UXC94315.1), and Lecanicillium aphanocladii polymycovirus 1 (XUP88367.1) (query cover = 99%; e-value = 1e-96). It contains a Pfam-annotated capsid protein domain, spanning amino acid residues 4–259, related to that of Colletotrichum camelliae filamentous virus 1 (PF25660, CcFV1_CP, e-value = 1.2e-95), indicating that it likely represents the capsid protein of the virus. dsRNA4 and dsRNA5 each contain a single ORF encoding proteins with predicted molecular masses of 36.26 kDa and 35.72 kDa, respectively, with no significant similarity to known proteins. Phylogenetic analysis of the VnaVM RdRp, along with homologous proteins encoded by recognized and putative polymycovirids and by hadaka virus 1, suggests that the virus studied in this work is a new member of the family Polymycoviridae . Based on the RdRp tree topology, VnaVM is evolutionarily closest to Trichoderma barbatum polymycovirus 1 and groups with other members of the newly established genus Multimycovirus (Fig. 2B). Similar results were obtained in phylogenetic analysis of the amino acid sequences of MTR and PASrp (not shown). Concluding remarks The phylogenetic position and genomic characteristics of VnaVM indicate its affiliation with the genus Multimycovirus in the family Polymycoviridae . Furthermore, VnaVM RdRp shares less than 58% sequence identity with homologs encoded by members of previously described species, which is below the proposed species demarcation threshold in the family (> 70% RdRp aa identity), supporting its classification as a novel member of this genus. To our knowledge, this is the first report of a polymycovirus infection in any Verticillium species. Given that some polymycoviruses influence the phenotype of their fungal hosts, ongoing research focuses on assessing the impact of VnaVM on host virulence. Declarations Funding This research was funded by the Slovenian Research and Innovation Agency (ARIS): postdoctoral research project Z4-50137 awarded to Vanja Miljanić, bilateral project Slovenia – United States of America BI-US/24-26-079, and research program P4-0077 Genetics and Modern Technologies of Crops. S.S. and N.A.-S. acknowledge partial support from the US Department of Agriculture, Hatch Projects 7006130 and 7006131. Author Contribution V.M. designed the experiments; S.R. provided fungal material; V.M. performed the experiments; V.M., S.S., N.A.-S., and J.J. analyzed the data; V.M. wrote the original draft; V.M., S.S., N.A.-S., J.J., S.R., and N.Š. reviewed and edited the manuscript. References Kanhayuwa L, Kotta-Loizou I, Özkan S, Gunning AP, Coutts RHA (2015) A novel mycovirus from Aspergillus fumigatus contains four unique dsRNAs as its genome and is infectious as dsRNA. 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Supplementary Files SupplementaryMaterialArchivesofVirology.docx SupplementaryFigure1.png Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 16 May, 2026 Reviews received at journal 14 May, 2026 Reviews received at journal 12 May, 2026 Reviewers agreed at journal 04 May, 2026 Reviewers agreed at journal 24 Apr, 2026 Reviewers invited by journal 23 Apr, 2026 Editor assigned by journal 21 Apr, 2026 Submission checks completed at journal 21 Apr, 2026 First submitted to journal 20 Apr, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9475253","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":633593023,"identity":"38fe8183-1f16-4252-8742-e63cd410a230","order_by":0,"name":"Vanja Miljanić","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABFUlEQVRIie2RvWrDMBRGbzDIi6jWa8hDXBNwO4T0VWQMnYwpBEKHDIaugb5N1igY7CVtH8CLp06eS4ZSqp8OpciZC9UBo2u4x98nDBAI/FFIP/wKFICEpYxrMMNNBDCrLyrMKXeSK6vgRcXC3NE4BQCnS3XP6v4M+zmbNW/DsH2tOPBsGABjEI03hU6VTHfQ62LtNcm2X2tFD6YY5l4lqUsibhWV6Z0+P+gUzD+N4r9L8jRS+mGV7l0rL/nOKDZFHL2KwJIWLuVkUtQPBfzFBI60mJNVNijbYs0jtrEKm7gLE2WWjg/9rcBun5y3q4rHj3qAZSFEN/gUY5H9mai+3yN3FGxi36y4b4lfLVbTRiAQCPwzvgD+BFILWsO0gwAAAABJRU5ErkJggg==","orcid":"","institution":"University of Ljubljana","correspondingAuthor":true,"prefix":"","firstName":"Vanja","middleName":"","lastName":"Miljanić","suffix":""},{"id":633593024,"identity":"8f08ad9a-04fc-4bd1-b675-6b663fad5674","order_by":1,"name":"Sead Sabanadzovic","email":"","orcid":"","institution":"Mississippi State University","correspondingAuthor":false,"prefix":"","firstName":"Sead","middleName":"","lastName":"Sabanadzovic","suffix":""},{"id":633593025,"identity":"2bd6a9d1-1683-4b2e-a4dc-ea58fc1dbd9c","order_by":2,"name":"Nina Aboughanem-Sabanadzovic","email":"","orcid":"","institution":"Mississippi State University","correspondingAuthor":false,"prefix":"","firstName":"Nina","middleName":"","lastName":"Aboughanem-Sabanadzovic","suffix":""},{"id":633593029,"identity":"979d6d6e-9377-496f-802b-58a388435d9f","order_by":3,"name":"Jernej Jakše","email":"","orcid":"","institution":"University of Ljubljana","correspondingAuthor":false,"prefix":"","firstName":"Jernej","middleName":"","lastName":"Jakše","suffix":""},{"id":633593030,"identity":"4853fdf1-eaf7-4772-b797-20ced71a80e7","order_by":4,"name":"Sebastjan Radišek","email":"","orcid":"","institution":"Slovenian Institute of Hop Research and Brewing","correspondingAuthor":false,"prefix":"","firstName":"Sebastjan","middleName":"","lastName":"Radišek","suffix":""},{"id":633593032,"identity":"6489d223-4c6e-47be-9e54-1f524f847f95","order_by":5,"name":"Nataša Štajner","email":"","orcid":"","institution":"University of Ljubljana","correspondingAuthor":false,"prefix":"","firstName":"Nataša","middleName":"","lastName":"Štajner","suffix":""}],"badges":[],"createdAt":"2026-04-20 17:24:41","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9475253/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9475253/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":108493530,"identity":"9e23b173-fae9-4e81-8232-3807f6078bf0","added_by":"auto","created_at":"2026-05-05 10:00:53","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":7807016,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003e(A)\u003c/strong\u003e Fungus \u003cem\u003eV. nonalfalfae\u003c/em\u003e isolate 274VD. \u003cstrong\u003e(B)\u003c/strong\u003edsRNA was electrophoresed in a 1% agarose gel. Lane 1, DNA ladder; lane 2, dsRNA without S1 nuclease and DNase I treatment; lane 3, dsRNA treated with S1 nuclease and DNase I. \u003cstrong\u003e(C) \u003c/strong\u003eSchematic representation of the genome organization of VnaVM RNAs 1 to 6. Pink boxes indicate ORFs, and black lines indicate UTRs.\u003c/p\u003e","description":"","filename":"Figure1.png","url":"https://assets-eu.researchsquare.com/files/rs-9475253/v1/0c7a55a371cb4d71d6463a41.png"},{"id":108419511,"identity":"b631481f-53c1-4c6c-a814-613db71f12b4","added_by":"auto","created_at":"2026-05-04 12:17:02","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":8406518,"visible":true,"origin":"","legend":"\u003cp\u003e(\u003cstrong\u003eA)\u003c/strong\u003e Amino acid sequence alignment of RdRp motifs IV-VI of Verticillium nonalfalfae virus M (VnaVM) and other members of the family \u003cem\u003ePolymycoviridae\u003c/em\u003e. StPmV1, Setosphaeria turcica polymycovirus 1; MoPmV1, Magnaporthe oryzae polymycovirus 1; AaPmV1, Alternaria alternata polymycovirus 1; MbPmV1, Metarhizium brunneum polymycovirus 1; BbPmV2, Beauveria bassiana polymycovirus 2; BbPmV3, Beauveria bassiana polymycovirus 3; TbPMV1, Trichoderma barbatum polymycovirus 1; AfuPmV-1, Aspergillus fumigatus polymycovirus 1; AfuTmV-1, Aspergillus fumigatus tetramycovirus 1; PjPmV1, Penicillium janthinellum polymycovirus 1. \u003cstrong\u003e(B)\u003c/strong\u003e Maximum-likelihood phylogenetic tree showing the relationships of VnaVM with members of the two families in the order \u003cem\u003eXenadelphovirales\u003c/em\u003e. VnaVM groups with members of the genus \u003cem\u003eMultimycovirus\u003c/em\u003e (clade shaded blue) in the family \u003cem\u003ePolymycoviridae\u003c/em\u003e. Clades corresponding to the other two genera in this family, \u003cem\u003ePolymycovirus\u003c/em\u003e and \u003cem\u003ePlurimycovirus,\u003c/em\u003e are shaded in green and yellow, respectively. The tree was constructed on the MAFFT-aligned [30] RdRp amino acid sequences using IQ-TREE v 3.0.1. [29] under best-fit model “Q.PFAM+F+I+G4” according to BIC, as estimated by ModelFinder [31]. The tree is visualized with iTOL v7 [32] . The GenBank accession numbers of RdRp amino acid sequences used for analysis along with virus names are indicated at the branch tips. Red dots at a branching point indicate statistical support \u0026gt;90%, with the size corresponding to the bootstrap value. The clade representing the second family in the order, \u003cem\u003eHadakaviridae\u003c/em\u003e, is collapsed.\u003c/p\u003e","description":"","filename":"Figure2.png","url":"https://assets-eu.researchsquare.com/files/rs-9475253/v1/411b4d4c3962ff0376684f4a.png"},{"id":109204416,"identity":"3709c3eb-3cdc-4984-870f-529751bdf6a3","added_by":"auto","created_at":"2026-05-13 14:59:28","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":15859538,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9475253/v1/8955d7a2-127a-4a41-b1f5-c8d54ccf8752.pdf"},{"id":108419509,"identity":"dad2fa0f-8962-4caa-802a-ebf9aae5701a","added_by":"auto","created_at":"2026-05-04 12:17:02","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":4822651,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryMaterialArchivesofVirology.docx","url":"https://assets-eu.researchsquare.com/files/rs-9475253/v1/a230b8e952c2c1dc06862ef3.docx"},{"id":108492897,"identity":"d62b055e-b53d-4c2a-be2c-f46c0ddf7163","added_by":"auto","created_at":"2026-05-05 09:58:55","extension":"png","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":4806758,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryFigure1.png","url":"https://assets-eu.researchsquare.com/files/rs-9475253/v1/173923c5c7288649b7f88fa5.png"}],"financialInterests":"No competing interests reported.","formattedTitle":" A novel hexasegmented virus isolated from the phytopathogenic fungus Verticillium nonalfalfae","fulltext":[{"header":"Introduction","content":"\u003cp\u003ePolymycovirids, viruses belonging to the \u003cem\u003ePolymycoviridae\u003c/em\u003e family, are a group of recently described mycoviruses. The first polymycovirid was discovered in the human pathogenic fungus \u003cem\u003eAspergillus fumigatus\u003c/em\u003e [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. Since then, polymycoviruses have also been identified in a range of plant- [\u003cspan additionalcitationids=\"CR3 CR4 CR5\" citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e] and insect-associated fungi [\u003cspan additionalcitationids=\"CR8 CR9\" citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. These viruses are notable for their unusual genomic structure, which typically comprises four to eight dsRNA segments, each containing one, or rarely two, putative open reading frames (ORFs) flanked by 5\u0026prime;- and 3\u0026prime;-untranslated regions (UTRs). All known polymycovirids share a \u0026ldquo;core\u0026rdquo; of four conserved genomic segments coding for RNA-dependent RNA polymerase (RdRp), a hypothetical cysteine-rich protein, putative methyltransferase (MTR), and a proline-alanine-serine-rich protein (PASrp) [\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. The family \u003cem\u003ePolymycoviridae\u003c/em\u003e, officially recognized in 2020 as a monogeneric, has recently been reorganized and expanded to include 28 species classified into three genera: \u003cem\u003ePolymycovirus\u003c/em\u003e, \u003cem\u003ePlurimycovirus\u003c/em\u003e and \u003cem\u003eMultimycovirus\u003c/em\u003e [\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e, \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. Evolutionarily, polymycovirids are distantly related to hadakavirids (family \u003cem\u003eHadakaviridae\u003c/em\u003e), both currently classified in the order \u003cem\u003eXenadelphovirales\u003c/em\u003e, class \u003cem\u003eMycopleornaviricetes\u003c/em\u003e [\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e, \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eSome polymycovirids have been shown to alter various host features, such as growth, pigmentation, sporulation, changes in virulence, fungicide susceptibility, and metabolic pathways [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e, \u003cspan additionalcitationids=\"CR15\" citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eVerticillium wilt, caused by ascomycete filamentous fungi in the genus \u003cem\u003eVerticillium\u003c/em\u003e, is among the most devastating plant vascular fungal diseases worldwide. Ten species are currently recognized in the genus \u003cem\u003eVerticillium sensu stricto\u003c/em\u003e (\u003cem\u003eV. albo-atrum\u003c/em\u003e, \u003cem\u003eV. alfalfae\u003c/em\u003e, \u003cem\u003eV. dahliae\u003c/em\u003e, \u003cem\u003eV. isaacii\u003c/em\u003e, \u003cem\u003eV. klebahnii\u003c/em\u003e, \u003cem\u003eV. longisporum\u003c/em\u003e, \u003cem\u003eV. nonalfalfae\u003c/em\u003e, \u003cem\u003eV. nubilum\u003c/em\u003e, \u003cem\u003eV. tricorpus\u003c/em\u003e, and \u003cem\u003eV. zaregamsianum\u003c/em\u003e) [\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. These soilborne fungi can persist in soil for several years without a host by producing melanized resting structures [\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. Following host-induced germination of these resting structures, the fungus penetrates the root system and subsequently colonizes the xylem, resulting in wilting and, in severe cases, complete dieback [\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eHop (\u003cem\u003eHumulus lupulus\u003c/em\u003e L.) is one of the most susceptible hosts to \u003cem\u003eV. nonalfalfae\u003c/em\u003e. Highly virulent or lethal pathotypes spread rapidly throughout hop plantations, resulting in significant yield losses and long-term impacts on hop production [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eTo date, a total of seven mycoviruses have been reported from \u003cem\u003eVerticillium\u003c/em\u003e spp.: Verticillium dahliae ormycovirus 1 (VdOMV1) and VdOMV2 (family \u003cem\u003eOrmycoviridae\u003c/em\u003e), Verticillium dahliae chrysovirus 1 (VdCV1; family \u003cem\u003eChrysoviridae\u003c/em\u003e), Verticillium dahliae partitivirus 1 (VdPV1; family \u003cem\u003ePartitiviridae\u003c/em\u003e), Verticillium dahliae magoulivirus 1 (VdMoV1; family \u003cem\u003eBotourmiaviridae\u003c/em\u003e), Verticillium dahliae RNA virus 1 (VdRV1; unassigned non-segmented\u0026thinsp;+\u0026thinsp;RNA virus), and Verticillium albo-atrum partitivirus 1 (VaaPV1; family \u003cem\u003ePartitiviridae\u003c/em\u003e) [\u003cspan additionalcitationids=\"CR22 CR23 CR24 CR25\" citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eIn this study, we report the molecular characterization of the novel hexasegmented virus from the phytopathogenic fungus \u003cem\u003eV. nonalfalfae\u003c/em\u003e isolate 274VD, tentatively named Verticillium nonalfalfae virus M (VnaVM).\u003c/p\u003e"},{"header":"Provenance of the virus material","content":"\u003cp\u003eThe source material for dsRNA isolation was \u003cem\u003eV. nonalfalfae\u003c/em\u003e isolate 274VD, recovered from an infected hop plant in Slovenia in 2024. Fungal mycelia were harvested from a pure culture grown at room temperature on a cellophane membrane overlaid on the surface of ½ Czapek Dox plate (supplemented with 1g/l of malt extract, 1 g/l of yeast extract, 1g/l of peptone, and 15 g/l of agar). DsRNA was extracted from fungal mycelium using cellulose C6288 (Sigma-Aldrich, St. Louis, MO, USA), following the method described by Mokhtari and Ali [\u003cspan class=\"CitationRef\"\u003e27\u003c/span\u003e] with minor modifications. The nature of the dsRNA was confirmed by digestion of the extracts with DNase I (New England BioLabs) and S1 nuclease (Thermo Fisher Scientific). The purified dsRNAs were analyzed in a 1% (wt/vol) agarose gel and visualized under UV light after staining with 0.1 µg/mL ethidium bromide. Six dsRNA segments ranging from 1 to 2.4 kb were observed. The library was constructed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) according to the manufacturer’s instructions. The yield and size distribution of the amplified cDNA library were determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). Sequencing was performed on the Ion GeneStudio S5 Prime System (Thermo Fisher Scientific). Adapter sequences were removed from raw reads before processing with bioinformatics tools CLC Genomic Workbench and Genomics Server (Qiagen). The Basic Local Alignment Search Tool (BLAST) of the National Center for Biotechnology Information (NCBI) was used for sequence comparisons. Potential open reading frames (ORFs) and putative conserved domains were predicted using ORF Finder and the Conserved Domain Database (CDD) of NCBI, respectively. Proteins with no matches in CDD, were further investigated using HHPred for remote homology and structure prediction via pairwise comparison of profile hidden Markov models (HMMs) [\u003cspan class=\"CitationRef\"\u003e28\u003c/span\u003e]. Phylogenetic analysis was conducted with the IQ-Tree v.3.0.1 [\u003cspan class=\"CitationRef\"\u003e29\u003c/span\u003e] on amino acid sequences of RdRp aligned with MAFFT v.7 [\u003cspan class=\"CitationRef\"\u003e30\u003c/span\u003e] under the best-fit model Q.PFAM + F+I+G4 identified by ModelFinder [\u003cspan class=\"CitationRef\"\u003e31\u003c/span\u003e]. The tree was visualized with iTOL v7 [\u003cspan class=\"CitationRef\"\u003e32\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eThe complete sequences of dsRNA1-6 were obtained by rapid amplification of cDNA ends (RACE) using the SMART™ RACE cDNA Amplification Kit (Takara). All amplified fragments were purified, cloned, and Sanger sequenced. The complete sequence of each dsRNA segment was determined by sequencing at least three independent clones. Primer sets used in this study are detailed in Supplementary Table\u0026nbsp;1. The six dsRNAs are deposited in the GenBank database under the accession numbers PX960521-PX960526.\u003c/p\u003e "},{"header":"Sequence properties","content":"\u003cp\u003eA total of six dsRNA segments were extracted from \u003cem\u003eV. nonalfalfae\u003c/em\u003e isolate 274VD (Fig.\u0026nbsp;1A) and visualized by agarose gel electrophoresis (Fig.\u0026nbsp;1B). Each dsRNA segment contained a single open reading frame on the coding strand flanked by UTRs (Fig.\u0026nbsp;1C). The 5′-UTRs of the coding strands of dsRNAs 1–6 range from 30 to 117 nt in length and share a highly conserved 30-nt sequence (ACAUGGGGGAACAAAAYHWUAUAWMYKYRC) (Supplementary Fig.\u0026nbsp;1A). The 3′-UTRs vary from 64 to 299 nt in length and lack extensive conserved elements. Secondary structure prediction revealed that the 5′ and 3′ terminal regions of all six dsRNAs fold into stem–loop structures, as exemplified by dsRNA1 (Supplementary Fig.\u0026nbsp;1B).\u003c/p\u003e\u003cp\u003eVnaVM dsRNA1 contains one large ORF1 (nt 31-2337), which encodes a protein of 768 amino acids (aa) with an estimated molecular mass (\u003cem\u003eMr\u003c/em\u003e) of 84.07 kDa. The 84K protein shares high amino acid identity with RdRps encoded by members of the family \u003cem\u003ePolymycoviridae\u003c/em\u003e, particularly with that encoded by Exserohilum turcicum polymycovirus 1 (XBY85583.1) and Setosphaeria turcica polymycovirus 1 (UMZ55610.1) (57.96%, query cover = 100%, e-value = 0.0). VnaVM RdRp contains the six conserved motifs, including the characteristic tetrapeptide GDNQ in motif VI (Fig.\u0026nbsp;2A). This tetrapeptide is also found in members of the mycoviral families \u003cem\u003ePolymycoviridae\u003c/em\u003e and \u003cem\u003eHadakaviridae\u003c/em\u003e, as well as in some (-)RNA viruses belonging to the order \u003cem\u003eMononegavirales\u003c/em\u003e [\u003cspan class=\"CitationRef\"\u003e33\u003c/span\u003e].\u003c/p\u003e\u003cp\u003eVnaVM dsRNA2 contains a single ORF (ORF2, nt 79-2172) that encodes a hypothetical protein of 697 aa (\u003cem\u003eMr\u003c/em\u003e 74.03 kDa) of unknown function, which shares conserved aa content (25–48%) with the corresponding proteins of polymycovirids and Hadaka virus 1.\u003c/p\u003e\u003cp\u003eThe third dsRNA of VnaVM is also monocistronic, with a coding region spanning nucleotides 47 to 1900 and encoding a protein of 617 aa (\u003cem\u003eMr\u003c/em\u003e 66.56 kDa). The ORF3-encoded protein shows the highest identity of ~ 48% with homologs encoded by dsRNA3 of Alternaria alternata polymycovirus 1 (QVK45098.1) and Plasmopara viticola lesion associated polymycovirus 1 (QHG11068.1) (query cover = 89%; e-value = 7e-149). CDD-based searches indicate that the ORF3 protein contains a conserved RsmD superfamily domain from amino acid positions 385 to 621 (COG0742; e-value = 1.15e-06), suggesting its enzymatic nature as a SAM-dependent methyltransferase.\u003c/p\u003e\u003cp\u003eThe sixth dsRNA contains a single ORF (ORF6, nt 118–906) encoding a 262-aa product (\u003cem\u003eMr\u003c/em\u003e 28.03 kDa) relatively rich in proline (8.02%), alanine (10.68%), and serine (8.02%). A BLASTp search showed that the ORF6 protein sequence shares 56.70% identity with PASrps of Beauveria bassiana polymycovirus 4 (QRF54816.1), Beauveria bassiana polymycovirus 4 − 2 (UXC94315.1), and Lecanicillium aphanocladii polymycovirus 1 (XUP88367.1) (query cover = 99%; e-value = 1e-96). It contains a Pfam-annotated capsid protein domain, spanning amino acid residues 4–259, related to that of Colletotrichum camelliae filamentous virus 1 (PF25660, CcFV1_CP, e-value = 1.2e-95), indicating that it likely represents the capsid protein of the virus.\u003c/p\u003e\u003cp\u003edsRNA4 and dsRNA5 each contain a single ORF encoding proteins with predicted molecular masses of 36.26 kDa and 35.72 kDa, respectively, with no significant similarity to known proteins.\u003c/p\u003e\u003cp\u003ePhylogenetic analysis of the VnaVM RdRp, along with homologous proteins encoded by recognized and putative polymycovirids and by hadaka virus 1, suggests that the virus studied in this work is a new member of the family \u003cem\u003ePolymycoviridae\u003c/em\u003e. Based on the RdRp tree topology, VnaVM is evolutionarily closest to Trichoderma barbatum polymycovirus 1 and groups with other members of the newly established genus \u003cem\u003eMultimycovirus\u003c/em\u003e (Fig.\u0026nbsp;2B). Similar results were obtained in phylogenetic analysis of the amino acid sequences of MTR and PASrp (not shown).\u003c/p\u003e"},{"header":"Concluding remarks","content":"\u003cp\u003eThe phylogenetic position and genomic characteristics of VnaVM indicate its affiliation with the genus \u003cem\u003eMultimycovirus\u003c/em\u003e in the family \u003cem\u003ePolymycoviridae\u003c/em\u003e. Furthermore, VnaVM RdRp shares less than 58% sequence identity with homologs encoded by members of previously described species, which is below the proposed species demarcation threshold in the family (\u0026gt;\u0026thinsp;70% RdRp aa identity), supporting its classification as a novel member of this genus. To our knowledge, this is the first report of a polymycovirus infection in any \u003cem\u003eVerticillium\u003c/em\u003e species. Given that some polymycoviruses influence the phenotype of their fungal hosts, ongoing research focuses on assessing the impact of VnaVM on host virulence.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003e \u003c/p\u003e"},{"header":"Declarations","content":"\u003ch2\u003eFunding\u003c/h2\u003e \u003cp\u003eThis research was funded by the Slovenian Research and Innovation Agency (ARIS): postdoctoral research project Z4-50137 awarded to Vanja Miljanić, bilateral project Slovenia \u0026ndash; United States of America BI-US/24-26-079, and research program P4-0077 Genetics and Modern Technologies of Crops. S.S. and N.A.-S. acknowledge partial support from the US Department of Agriculture, Hatch Projects 7006130 and 7006131.\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eV.M. designed the experiments; S.R. provided fungal material; V.M. performed the experiments; V.M., S.S., N.A.-S., and J.J. analyzed the data; V.M. wrote the original draft; V.M., S.S., N.A.-S., J.J., S.R., and N.Š. reviewed and edited the manuscript.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eKanhayuwa L, Kotta-Loizou I, \u0026Ouml;zkan S, Gunning AP, Coutts RHA (2015) A novel mycovirus from \u003cem\u003eAspergillus fumigatus\u003c/em\u003e contains four unique dsRNAs as its genome and is infectious as dsRNA. 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Proc Natl Acad Sci USA 111(33):12205\u0026ndash;12210. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1073/pnas.1401786111\u003c/span\u003e\u003cspan address=\"10.1073/pnas.1401786111\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"archives-of-virology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"arvi","sideBox":"Learn more about [Archives of Virology](https://www.springer.com/journal/705)","snPcode":"705","submissionUrl":"https://submission.nature.com/new-submission/705/3","title":"Archives of Virology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-9475253/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9475253/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn this study, we describe a novel virus, tentatively named Verticillium nonalfalfae virus M (VnaVM), identified in the phytopathogenic fungus \u003cem\u003eVerticillium nonalfalfae\u003c/em\u003e isolated from hop (\u003cem\u003eHumulus lupulus\u003c/em\u003e L.). The VnaVM genome consists of six double-stranded RNA (dsRNA) segments ranging from 1051 to 2401 bp. DsRNAs 1\u0026ndash;3 encode an RNA-dependent RNA polymerase (RdRp), a hypothetical protein and a methyltransferase (MTR), respectively, while dsRNA6 code for a proline-alanine-serine-rich protein (PASrp). These four proteins share less than 58% amino acid identity with homologs encoded by viruses in the family \u003cem\u003ePolymycoviridae\u003c/em\u003e. Products encoded by dsRNAs 4 and 5 show no similarity to known proteins, and their roles in the viral life cycle are unknown. Phylogenetic analysis of RdRp amino acid sequences, along with other results, support classifying VnaVM as a representative of a novel species within the genus \u003cem\u003eMultimycovirus\u003c/em\u003e (family \u003cem\u003ePolymycoviridae\u003c/em\u003e). To our knowledge, this is the first report of a polymycovirid infection in a member of the genus \u003cem\u003eVerticillium\u003c/em\u003e.\u003c/p\u003e","manuscriptTitle":" A novel hexasegmented virus isolated from the phytopathogenic fungus Verticillium nonalfalfae","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-05-04 12:16:50","doi":"10.21203/rs.3.rs-9475253/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2026-05-16T12:14:16+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-14T20:46:13+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-12T14:04:48+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"96264249844824832079167556313157728998","date":"2026-05-05T03:48:29+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"36836536814247125444998991610857486308","date":"2026-04-24T10:38:23+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2026-04-23T11:10:28+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2026-04-21T07:31:03+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2026-04-21T07:30:57+00:00","index":"","fulltext":""},{"type":"submitted","content":"Archives of Virology","date":"2026-04-20T17:19:23+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"archives-of-virology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"arvi","sideBox":"Learn more about [Archives of Virology](https://www.springer.com/journal/705)","snPcode":"705","submissionUrl":"https://submission.nature.com/new-submission/705/3","title":"Archives of Virology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"a52c3315-bfe2-421d-8a70-06d321d0b382","owner":[],"postedDate":"May 4th, 2026","published":true,"recentEditorialEvents":[{"type":"decision","content":"Revision requested","date":"2026-05-16T12:14:16+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-14T20:46:13+00:00","index":17,"fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-12T14:04:48+00:00","index":16,"fulltext":""},{"type":"reviewerAgreed","content":"96264249844824832079167556313157728998","date":"2026-05-05T03:48:29+00:00","index":14,"fulltext":""}],"rejectedJournal":[],"revision":"","amendment":"","status":"in-revision","subjectAreas":[],"tags":[],"updatedAt":"2026-05-16T12:25:16+00:00","versionOfRecord":[],"versionCreatedAt":"2026-05-04 12:16:50","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-9475253","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-9475253","identity":"rs-9475253","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}