Tyrosinase-based proximity labeling in living cells and in vivo

Abstract Proximity labeling (PL) has emerged as a promising technology for spatial proteomics. However, most enzymes adopted for PL still have poor biocompatibility in vivo or suffer from slow or high-background labeling. We introduce a bacterial tyrosinase, BmTyr, as a new PL enzyme that addresses these limitations. BmTyr can be genetically fused or ligand-guided to a protein-of-interest in living cells and in vivo, unveiling its surrounding proteome efficiently by PL-based proteomics.