Influence of calcium nanoparticles on antioxidant enzymes, total carbohydrates content and isozymes of in vitro cultured Lupinus termis under cadmium stress.

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Calcium nanoparticles reduced cadmium's harmful effects on Lupinus termis callus by decreasing peroxidase and catalase activity, increasing starch, and altering isozyme profiles.

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This preprint studied whether calcium nanoparticles made from Ca(OH)2 or CaO (100 and 50 µM) could influence biochemical stress responses in in vitro cultured Lupinus termis callus exposed to cadmium (CdCl2 at 20 or 60 mg/L). In B5 medium after 30 days, cadmium-reduced antioxidant enzyme activities (peroxidase and catalase), while calcium nanoparticles decreased cadmium’s harmful effects by reducing those enzyme activities, and starch content increased only when Ca nanoparticles were added under the 60 mg/L Cd condition. Isozyme profiling showed two peroxidase isoforms and three amylase isoforms that varied in intensity/thickening across treatments. As an unreviewed Research Square preprint, the work’s limitations are not stated in the provided text. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Abstract Tissue culture technique was carried out in order to assess the possible effects of calcium nanoparticles either Ca(OH) 2 or CaO at concentration of 100 and 50 µM, respectively on peroxidase and catalase enzymes, total soluble carbohydrates content, starch content and isozymes of peroxidase and amylase of Lupinus termis callus at 30 days of growth. The experimental plant tissues were grown in culture using B 5 medium amended with either different concentrations of CdCl 2 (20 and 60 mg/l) or calcium nanoparticles either Ca(OH) 2 or CaO at concentrations of 100 and 50 µM, respectively. Ca nanoparticles decreased the harmful effects of Cadmium as the activities of peroxidase (POD) and catalase (CAT) were reduced by applying nanoparticles either Ca(OH) 2 or CaO to media having different concentrations of Cd. The content of Starch Tissue culture technique was carried out on Lupinus termis plant.was increased only by addition of Ca nanoparticles either Ca(OH) 2 or CaO and their mixture to 60 mg/l Cd. Isozyme profile of peroxidase and amylase in callus showed 2 isoforms of POD and three isoforms of amylase varied in their intensity and thickening in response to different treatments.
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Influence of calcium nanoparticles on antioxidant enzymes, total carbohydrates content and isozymes of in vitro cultured Lupinus termis under cadmium stress. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Influence of calcium nanoparticles on antioxidant enzymes, total carbohydrates content and isozymes of in vitro cultured Lupinus termis under cadmium stress. Basma M.M. Khalaf, Awatif A. Mohsen, Maged El-Kemary, Samha M.A. Dowidar, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-2167818/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Tissue culture technique was carried out in order to assess the possible effects of calcium nanoparticles either Ca(OH) 2 or CaO at concentration of 100 and 50 µM, respectively on peroxidase and catalase enzymes, total soluble carbohydrates content, starch content and isozymes of peroxidase and amylase of Lupinus termis callus at 30 days of growth. The experimental plant tissues were grown in culture using B 5 medium amended with either different concentrations of CdCl 2 (20 and 60 mg/l) or calcium nanoparticles either Ca(OH) 2 or CaO at concentrations of 100 and 50 µM, respectively. Ca nanoparticles decreased the harmful effects of Cadmium as the activities of peroxidase (POD) and catalase (CAT) were reduced by applying nanoparticles either Ca(OH) 2 or CaO to media having different concentrations of Cd. The content of Starch Tissue culture technique was carried out on Lupinus termis plant.was increased only by addition of Ca nanoparticles either Ca(OH) 2 or CaO and their mixture to 60 mg/l Cd. Isozyme profile of peroxidase and amylase in callus showed 2 isoforms of POD and three isoforms of amylase varied in their intensity and thickening in response to different treatments. Tissue culture Cadmium stress Nanoparticles Ca(OH)2 CaO Lupinus termis Biochemical changes. Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-2167818","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":156790117,"identity":"c767880e-4a40-43f8-9378-f933be41becf","order_by":0,"name":"Basma M.M. 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