Applicability of Commercial Clinical Chemistry Test Kits For Horse Serum

Abstract Background: Validation of a test method to determine its fitness for a particular use is critical for confirming that the test can generate accurate and precise data. Although several commercial biochemical test kits exist and validated for humans there is no specific and validated commercial clinical chemistry test kits designed for horse Objective: The aim of this study was to validate commercial clinical chemistry test kits designed for human serum for use in horses to accurately measure total cholesterol urea and total protein in serumMethods: This study used 25 apparently healthy adult horses. Blood samples were collected and pooled serum was prepared. Analytical validation comprises replication and recovery experiments. Total observable error (TEo%), Sigma metrics and quality goal index (QGI) was used to support the analytical validation studies.Result: Intra- and inter-assay variability was 2.05% and 2.08%, 2.26% and 1.89%, 2.4% and 1.63%, for total cholesterol urea and total protein for respectively; recovery was 99.46%, 97.32% and 100.1% for total cholesterol urea and total protein for respectively. TEo% for the specified analytes were within the allowable error indicated ASVCP guidelines. All the three anaytes satisfied the recommended requirement (>3 sigma values). The QGI for urea, as it had below 6σ was 0.95 indicating imprecision and inaccuracy.Conclusion: The results of the research endorse the suitability of the studied commercial clinical chemistry test kits and illustrated the acceptance criteria for serum collected from horse.