An Optimised Method for Robust Golgi–Cox Staining in Cortical Neurons

Golgi-based staining remains a valuable method for visualising the full morphology of individual neurons, however conventional workflows can be slow, variable, and technically demanding. Here, we present an optimised staining protocol designed to improve the reliability and usability of Golgi-based labelling in mouse cortical tissue. The revised workflow combines controlled fixation, dichromate impregnation, silver development, vibratome sectioning, and slide processing conditions that support consistent visualisation of neuronal somata and dendritic architecture. In the tissue examined here, the protocol produced clear impregnation with low background staining and was suitable for morphological assessment using standard light microscopy and image analysis software. Overall, this method provides a practical approach for laboratories seeking a straightforward and cost-conscious protocol for neuronal morphology