Network Pharmacology-based Analysis on Treatment of Diabetic Retinopathy by Salidroside

Objective: To study the mechanism of salidroside in the treatment of diabetic retina injury. Methods: : The animal model of diabetic retinal injury was established, salidroside treatment group was treated with salidroside, and then OCT detection, water maze detection, heat map difference analysis, molecular docking, PPI protein interaction, GO and KEGG analysis were used to study the protective mechanism of salidroside against diabetic retinal injury. Results: : OCT and water maze results showed that the learning and cognitive functions of rats in the DM group and sham group were significant on day 1 and 5 after salidroside treatment. Venny intersection map was made for the data screened by proteomic analysis, and heat map was made for the three intersection genes. It was found that Nsmce1, Inpp5f and Vcan genes were all up-regulated in the diabetes group. By establishing molecular docking between the intersection gene and salidroside, Vcan did not docking with salidroside ligand, and BOTH Nsmce1 and Inpp5f could establish stable molecular binding mode with salidroside. Through keyword search in PubMed, the associated gene combination and intersection genes were finally obtained, and PPI protein interaction was analyzed. The genes with mutual relationship were analyzed by GO enrichment. It was found that response to molecule of Bacterial Origin is the most important BP. The most important CC process is raft and cytokine receptor binding. KEGG analysis found that the most important signaling pathways were Lipid and atherosclerosis. Conclusion: Salidroside has certain therapeutic effect on diabetic retina injury.