Molecular Analytics of Biosurfactant Producing Bacteria Isolated from Soil

Molecular Analytics of Biosurfactant Producing Bacteria Isolated from Soil | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Molecular Analytics of Biosurfactant Producing Bacteria Isolated from Soil Frederick Mintah Appiah This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9372462/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Biosurfactant products show significant potential compared to chemical surfactants due to their biodegradability, low toxicity, and eco-friendliness. This study aims to characterize the structures of biosurfactants produced by bacteria isolated from soil and to evaluate their potential in degrading petrol and oil. Biosurfactant-producing strains were selected using oil spreading tests, screening for petrol-degrading bacteria, antimicrobial susceptibility tests, and PCR-based phylogenetic analysis. In total, 5852 colonies and strains of biosurfactant producers were isolated through cultural and molecular methods. These bacteria were classified into three species: Bacillus, Pseudomonas, and Staphylococcus. After biodegradation, the effects of petrol and olive oil, combined with cell-free supernatant, were analyzed, showing an efficient removal rate of 79.59% after 24 hours. Among the bacterial isolates, three strains demonstrated significant oil degradation capacity: Bacillus sp. With a degradation rate of 79.59%, Pseudomonas sp. At 77.55%, and Staphylococcus sp. At 67.3%. In conclusion, biosurfactant-producing bacteria were isolated from soil samples collected at the Center for Bioscience and Nanoscience Research Laboratory through screening and isolation efforts. Analytical Biochemistry Biochemical Research Methods Behavioral Ecology Bacteriology General Microbiology Molecular Genetics Dermatology Biosurfactants Soil Bacteria Petrol oil Olive oil Degradation PCR Full Text Additional Declarations The authors declare potential competing interests as follows: I'm the sole author of this paper and only the college and department are affiliated. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9372462","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":620561237,"identity":"8faaa7d4-6895-4a2e-975c-cb575aff3ef6","order_by":0,"name":"Frederick Mintah Appiah","email":"data:image/png;base64,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","orcid":"https://orcid.org/0009-0009-7619-0481","institution":"Rathinam College of Arts and Science","correspondingAuthor":true,"prefix":"","firstName":"Frederick","middleName":"Mintah","lastName":"Appiah","suffix":""}],"badges":[],"createdAt":"2026-04-09 21:23:11","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":true,"conflictsOfInterestStatement":true,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":true},"doi":"10.21203/rs.3.rs-9372462/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9372462/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":106728482,"identity":"f34d1028-14bc-414b-824d-5c4101d27641","added_by":"auto","created_at":"2026-04-12 18:42:57","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1908372,"visible":true,"origin":"","legend":"","description":"","filename":"FrederickMintahBMBThesisReview.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9372462/v1_covered_2a9d6f7d-5cf5-40ce-b7cb-18b5fc2dc757.pdf"}],"financialInterests":"The authors declare potential competing interests as follows: I'm the sole author of this paper and only the college and department are affiliated. 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