Halocynthiibacter halioticoli sp. nov., isolated from the abalone Haliotis discus hannai

A Gram-stain-negative, rod-shaped, glide, non-flagellated, and facultatively aerobic bacterial strain, designated as Z654 T , was isolated from the gut of abalone Haliotis discus hannai from Rongcheng, Shandong province, China. Cells are 0.2–0.8 µm in width and 0.7–3.4 µm in length. Cells grew best at 30°C (range, 15–37°C), pH 7 (range, 6.0–8.5) and NaCl concentration of 2.0% (w/v) (range, 1–10%). According to the phylogenetic analysis of 16S rRNA gene sequence, the strain belongs to the genus Halocynthiibacter and the most close strain is Halocynthiibacter arcticus KCTC 42129 T (97.12%). The genome size of strain Z654 T was 3296250 bp and the DNA G + C content was 54.2 mol%. The average nucleotide identity (ANI) scores and digital DNA–DNA hybridization (dDDH) scores with H. arcticus KCTC 42129 T was 70% and 14.6–18.2% respectively. The predominant quinone was Q-10 and the major fatty acids were C 18:0 , C 18:1 ω 7 c 11-methyl and summed future 8. The polar lipids were consisted of phosphatidylcholine, phosphatidylglycerol, unidentified aminolipid and unidentifed lipids. Based on the phenotypic, phylogenetic and chemotaxonomic data, strain Z654 T was considered to represent a novel species of the genus Halocynthiibacter , for which the name Halocynthiibacte halioticoli sp. nov. , is proposed. The type strain is Z654 T (= MCCC 1H00503 T  = KCTC 92003 T ).