Quantification of mRNA levels of OCT4 and TGF-β receptor I (TGF-β RI) in human endometrial tissues.

Reverse transcription and quantitative real-time PCR were used to quantify the levels of (A)OCT4 and (B)TGF-β RI mRNAs in RL95-2 human endometrial cell lines, primary endometriotic stromal cells, normal endometrium and hyperplastic endometrium (n = 8), adenomyosis (n = 23), and chocolate cysts (n = 24). The RL95-2 cells were used as internal controls for normalization. Results are expressed as mean ± SD. **P < .01, ***P < .001 in comparison with the control group of normal and hyperplastic endometrium tissues. By Mann-Whitney-U test.