Cultivation of Bovine Lipid Chunks on Aloe vera Scaffolds

Cultivation of Bovine Lipid Chunks on Aloe vera Scaffolds | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Cultivation of Bovine Lipid Chunks on Aloe vera Scaffolds Gilad Gome, Benyamin Chak, Shadi Tawil, Itai Rotem, Ivana Ribarski-Chorev, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4630482/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 25 Feb, 2025 Read the published version in npj Science of Food → Version 1 posted 10 You are reading this latest preprint version Abstract Aloe vera, renowned for its medicinal and food applications, presents a sustainable, scalable, and cost-effective alternative for cultured meat production. Our innovative method repurposes the Aloe vera parenchyma to produce sterile scaffolds with a conducive porous structure that allows liquid retention. These scaffolds, demonstrating good biocompatibility, support adhesion, proliferation, and extracellular matrix formation of bovine mesenchymal stem cells. The addition of oleic acid leads to the accumulation of lipids, indicating the potential formation of ’lipid chunks’. This cultured bovine 3D tissue, enriched with lipids, can serve as a food additive in plant-based alternative meats, potentially enhancing their texture and flavor profile. To address the scalability challenge, a novel macrofluidic single use bioreactor (MSUB) was used to culture the scaffolds as an example of the potential of various scaling out or up possibilities, enabling sustainable and scalable production of cultured meat or to be used for regenerative medicine for cell-based therapies. This breakthrough in Aloe vera scaffold utilization not only advances the field of tissue engineering but also holds promise for a more sustainable future in alternative protein production. Biological sciences/Stem cells/Mesenchymal stem cells Biological sciences/Biotechnology/Environmental biotechnology Biological sciences/Cell biology/Cell division/Cell growth Tissue Engineering Cultured Meat Scaffolds Aloe vera Adipose Stem cells Full Text Additional Declarations (Not answered) Supplementary Files FigureS1.pdf Fabrication of macrofluidic single use bioreactor for Aloe vera scaffold cultivation Sup1video.mp4 Aloe vera scaffolds with cells in MSUB Cite Share Download PDF Status: Published Journal Publication published 25 Feb, 2025 Read the published version in npj Science of Food → Version 1 posted Editorial decision: revise 07 Nov, 2024 Review # 3 received at journal 04 Nov, 2024 Reviewer # 3 agreed at journal 16 Oct, 2024 Review # 2 received at journal 04 Aug, 2024 Reviewer # 2 agreed at journal 17 Jul, 2024 Reviewer # 1 agreed at journal 17 Jul, 2024 Reviewers invited by journal 17 Jul, 2024 Submission checks completed at journal 01 Jul, 2024 Editor assigned by journal 24 Jun, 2024 First submitted to journal 24 Jun, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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