Acanthopanax senticosus polysaccharides attenuates Klebsiella infection in immunocompromised mice by modulating immunity

Acanthopanax senticosus polysaccharides attenuates Klebsiella infection in immunocompromised mice by modulating immunity | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Acanthopanax senticosus polysaccharides attenuates Klebsiella infection in immunocompromised mice by modulating immunity Zihan Liang, Yiyao Wang, Jingyi Zhang, Yixiang Lai, Lanlan Yin, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4283450/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Radiotherapy and chemotherapy compromise individuals’ immunity clinically, resulting in increased opportunistic infections. In this study, the cyclophosphamide-induced immunocompromised C57BL/6J mouse with Klebsiella infection was hired to analyze the effect of the active components of Acanthopanax senticosus on anti-infection for immunocompromised individuals. As a result, Acanthopanax senticosus polysaccharide (ASPS) was selected with the best effect. For immunocompromised C57BL/6J mouse, ASPS increased its indices of thymus and spleen, reducing the bacteria load in lung and protecting the function of liver. Meanwhile, ASPS obviously boosted the total number of thymocytes and the number of CD4+ T cells in spleen, promoting the proportions of CD4+ TCM and TEM cells, while decreasing that of CD4+ TEMRA cells in in peripheral blood. Moreover, ASPS enhanced the number of NF-κB+, CD192+ Mφ and NF-κB+ neutrophils in the abdomen and strengthened their antibacterial function, however, reduced the number of NF-κB+ Mφ and neutrophils in the spleen reducing inflammation. It is also observed by immunofluorescence that the NF-κB expression of peritoneal Mφ increased evidently after the mice were treated with ASPS. In conclusion, ASPS has an obvious effect against infection for immunocompromised mice by promoting immunity recovery. Acanthopanax senticosus polysaccharide compromised immunity Klebsiella pneumoniae infection immune cells Full Text Additional Declarations No competing interests reported. Supplementary Files FigureS1GatingstrategiesofTECscellsandtheirsubsets.jpg FigureS2Gatingstrategiesofthymocytes.jpg FigureS3GatingstrategiesofTcellsinspleen.jpg FigureS4GatingstrategiesofTcellsinperipheralblood.jpg FigureS5Gatingstrategiesofneutrophilsandmonocytesinperipheralblood.jpg FigureS6GatingstrategiesofMandneutrophilsinabdomen.jpg FigureS7GatingstrategiesofMandneutrophilsinspleen.jpg Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4283450","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":292983700,"identity":"332db7ea-c6b8-4c5e-b787-3e4412d60a68","order_by":0,"name":"Zihan Liang","email":"","orcid":"","institution":"China Three Gorges University","correspondingAuthor":false,"prefix":"","firstName":"Zihan","middleName":"","lastName":"Liang","suffix":""},{"id":292983701,"identity":"77c71cdb-7e0e-47de-9479-629a8558ee7b","order_by":1,"name":"Yiyao Wang","email":"","orcid":"","institution":"China Three Gorges 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