Paenibacillus xylinteritussp. nov., a novel bacterial species isolated from grapevine wood

ABSTRACT Bacteria naturally colonize grapevine wood, and some populations interact synergistically with fungal pathogens to promote wood degradation. In this study, we characterized a bacterial strain, designated S150 T , isolated from the wood of grapevine cultivar Sauvignon Blanc. The strain was previously known as promoting wood degradation caused by the causal agent of white-rot, Fomitiporia mediterranea . Phylogenetic analysis based on the full-length 16S rRNA gene sequence showed that the strain S150 T belonged to the genus Paenibacillus . Based on 16S rRNA gene sequences, S150 T shared low sequence similarity values (between 96.5 and 97.5%) with closed recognized members of the genus ( P. jilunlii Be17, P. tepidiphilus SYSU G01001, P. tengchongensis SYSU-G-01003, P. sonchi X19-5, P. helianthi P26E). The cell wall peptidoglycan of S150 contains meso-diaminopimelate. MK6 and MK7 are the two respiratory quinones. The main cellular fatty acids are iso-C 16:0 and anteiso-C 15:0 . The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, three amino phospholipids, two phospholipids and three polar lipids. The whole-genome is 7.45 Mb, with a G+C content of 52.54%. The average nucleotide identity (ANI) value between S150 T and the closely related Paenibacillus member ( P. sonchi X19-5) was 85.06%. Biochemical and physiological analyses revealed that strain S150 T has different characteristics from reference strains Paenibacillus spp.. Phenotypic, phylogenetic and chemotaxonomic analyses show strain S150 T is a novel species of the genus Paenibacillus , for which the name Paenibacillus xylinteritus is proposed.