Differential gene expression between urban and rural acorn ant populations

preprint OA: closed CC-BY-4.0

Abstract

The acorn ant, Temnothorax curvispinosus, is a model system for rapid evolution of physiological traits to urban environments. Here, we performed a transcriptome-wide comparison of changes in gene expression between urban and rural populations of acorn ants in the southeastern United States. Our analyses revealed 287 differentially expressed genes. Overrepresentation in gene ontology terms was consistent with evolved differences in whole-organism traits such as metabolism and running speed. Transcriptome-wide comparisons also implicated an important role for cuticle development, which could directly aid in maintaining water balance in urban environments, with potential indirect effects on heat tolerance.
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This is a Preprint and has not been peer reviewed. This is version 1 of this Preprint. You must log in to post a comment. There are no comments or no comments have been made public for this article. This is a Preprint and has not been peer reviewed. This is version 1 of this Preprint. Add a Comment You must log in to post a comment. Comments There are no comments or no comments have been made public for this article. The acorn ant, Temnothorax curvispinosus, is a model system for rapid evolution of physiological traits to urban environments. Here, we performed a transcriptome-wide comparison of changes in gene expression between urban and rural populations of acorn ants in the southeastern United States. Our analyses revealed 287 differentially expressed genes. Overrepresentation in gene ontology terms was consistent with evolved differences in whole-organism traits such as metabolism and running speed. Transcriptome-wide comparisons also implicated an important role for cuticle development, which could directly aid in maintaining water balance in urban environments, with potential indirect effects on heat tolerance. https://doi.org/10.32942/X2PW96 Life Sciences Published: 2026-03-08 00:33 Last Updated: 2026-03-08 00:33 CC BY Attribution 4.0 International Data and Code Availability Statement: https://doi.org/10.17605/OSF.IO/2R5SH Language: English

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License: CC-BY-4.0