L1 gene based Molecular Characterization of Bovine papillomavirus type 1 (BPV1) isolated from cutaneous warts of cattle, Maharashtra (India)

Bovine Papillomatosis (BP) is a contagious disease of the animals in which it naturally occurs. It is caused by the bovine papillomavirus (BPV) and is characterized by warts that occur in cutaneous and mucosal forms. Cattle are affected by six different types of papillomaviruses. BPV-1 and − 2 infections can cause warts on the skin, teats, udders, penis, and tumors in the urinary bladder. In the present study, total of 10 wart samples from cattle were studied. PCR was performed to detect the presence of BPV and its type. Further, the PCR amplified product was sent for the sequencing for the confirmation and phylogeny analysis. The result revealed that the cutaneous warts of six cattle were positive for BPV-1 while all the samples were found negative for BPV-2. BPV-1 was detected in cutaneous warts by a PCR and was confirmed by nucleotide sequencing and phylogenetic analysis. In conclusion, the BPV type prevalent in the cutaneous warts of Indian cattle is the BPV-1 type.