Microbial biotemplates for obtaining silica porous carrier for antibacterial substance

Microbial biotemplates for obtaining silica porous carrier for antibacterial substance | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Microbial biotemplates for obtaining silica porous carrier for antibacterial substance Elizaveta A. Lantsova, Violetta A. Pertseva, Denis A. Kolykhalov, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8310871/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 7 You are reading this latest preprint version Abstract This study proposes an alternative to the template method for forming porous silica carriers. Bacterial cells of Rhodococcus fascians VKM B-1462 and Pseudomonas veronii VKM B-877 were utilized as biotemplates for the synthesis of a material based on tetraethoxysilane and methyltriethoxysilane (50/50 vol. %). As comparison samples, type III and IV materials were synthesized using cetyltrimethylammonium bromide (CTAB) micelles as templates, using methyltriethoxysilane and tetraethoxysilane 50/50 vol.%, as well as 100 vol.% tetraethoxysilane. The templates were removed from the materials by high-temperature annealing in the range from 200 to 1200 °C. The formation of a silicon-containing matrix was confirmed by scanning electron microscopy and IR spectroscopy. The optimal temperature for the removal of templates from materials was determined to be 800 °C for all types of material. An antiseptic substance, octenidine dihydrochloride, was loaded onto silicon carriers. UV spectroscopy revealed that the matrix formed using the Rhodococcus fascians biotemplate demonstrated optimal sorption and desorption properties. In comparison with the CTAB template, the employment of microbial cells has the potential to offer a number of applications due to cost-effectiveness, environmental safety, and ease of removal of the pore-forming agent. sol-gel silicon сarrier biotemplate octenidine dehydrochloride antibacterial porous material Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 1. Introduction Porous silicate materials represent a highly versatile class of inorganic matrices, which are distinguished by their ability to exhibit tunable texture, surface chemistry, and hierarchical porous structure [ 1 – 3 ]. At present, there is an increased demand for effective catalysts, adsorbents, membrane and filtration elements, sensors, drug delivery systems and materials for the energy sector, where controlled porosity and stability play a key role [ 4 – 7 ]. Sol-gel technology is a convenient method for synthesizing materials with customisable properties. It is characterized by its simplicity, environmentally friendly and rapid synthesis of non-toxic matrices, and the ability to obtain a variety of materials, such as thin films, membranes, powders, etc. [ 8 – 10 ]. Sol-gel material has been found to exhibit a number of advantageous properties, which have resulted in its extensive utilization in a variety of fields. These include medicine, ecology, electrochemistry, and the formation of catalysts and biocatalysts [ 11 – 17 ]. A notable area of interest lies in the utilization of sol-gel for the fabrication of sorbents [ 18 – 21 ], carrier materials for antimicrobial substances [ 22 – 24 ] and systems for delivering medicines. In order to obtain materials with controlled release of the substance, it is important to have a sufficiently large surface area and a developed pore system for diffusion of the active component. The template method, a widely utilized technique, facilitates the synthesis of micro- and mesoporous materials, characterized by an extensive surface area. However, the diffusion of the active substance may be limited by the presence of small-diameter pores, a phenomenon that is particularly pronounced when a sufficiently sterically loaded molecule is desorbed [ 25 ]. Furthermore, cetylmethyltrimonium bromide (CTAB) is a frequently utilized template. This quaternary ammonium compound functions as a surfactant [ 26 , 27 ]. However, CTAB is a toxic substance and may not be fully removed even by high-temperature treatment [ 28 ]. Furthermore, during the synthesis of materials, there is a possibility of environmental accumulation, which would have a detrimental effect on the environment. Microorganism cells have been found to serve as an alternative to surfactant templates in the formation of porous materials. These cells are characterized by a wide variety of shapes, similar morphology among cells of the same strain, reproducibility of cell structure, environmental friendliness, and ease of biomass reproduction [ 29 , 30 ]. In order to utilize microbial cells as templates, it is necessary to first encapsulate them completely in organosilicon material, and then to anneal them to form pores of a size corresponding to that of the microbial cells (typically 1–3 micrometres). The sol-gel material formed around the microorganism cells has a developed porous structure, which has been previously studied in the process of forming a number of bioreceptor elements for the formation of biosensors [ 11 , 31 ]. The development of a pore system enables the encapsulation of microorganisms within matrices, thereby preserving their catalytic activity and facilitating the acquisition of substrates through diffusion. This process also enables the elimination of waste products, thereby extending the operational lifespan of biosensors without the necessity of replacing the biomaterial. It has previously been established that each cell is immobilized individually, which is important for their use as templates [ 32 , 33 ]. Gram-positive and gram-negative bacteria of various shapes can be used as templates. Rhodococcus fascians ( R. fascians ) are gram-positive, non-spore-forming bacteria measuring 1.0-2.5*0.5-1.0 µm, and capable of pleomorphism. In the exponential phase of active growth, the bacteria exhibit an elongated rod-like morphology. In the stationary phase, the bacteria acquire an oval shape, which is the predominant form for this particular type of microorganism. R. fascians possesses a Gram-positive cell wall that exhibits characteristics consistent with those of mycobacteria, including the presence of mycolic acids. These acids have been demonstrated to facilitate the adsorption of precursor molecules and function as effective nucleation centres within the sol-gel process. Another template employed is Pseudomonas veronii , a Gram-negative rod-shaped non-spore-forming bacterium measuring 0.5-1.0 * 1.5-5.0 µm. The stable, rod-shaped form and small diameter of the fibres enable the creation of materials with a high specific surface area and an elongated, fibrous architecture. The outer membrane of P. veronii , which is rich in lipopolysaccharides, provides a multitude of hydroxyl and carboxyl groups that act as centres for the chemical binding of organosilicon precursors. The utilization of Gram-positive and Gram-negative bacteria has been observed to result in the formation of matrices exhibiting diverse morphologies. This phenomenon has been demonstrated to impact the sorption and desorption properties of the matrices. The application of cells as biotemplates for the synthesis of porous carrier materials has the potential to enhance the sorption characteristics of matrices and mitigate diffusion-related challenges. This approach facilitates the regulated release of diverse active substances, including octenidine dihydrochloride, thereby achieving an antibacterial effect. Numerous studies utilize benzalkonium chloride [ 34 , 35 ] and cetylpyridinium chloride as active agents [ 36 , 37 ]. However, these compounds were initially described in the 1930s and are still widely employed today, despite their rapid loss of effectiveness due to the development of bacterial resistance [ 38 – 40 ]. The choice of octenidine as an antibacterial agent is predicated on several factors. Firstly, it is non-toxic, which makes it a suitable agent for use in humans. Secondly, it is capable of effectively combating both Gram-positive and Gram-negative microorganisms [ 41 , 42 ]. The material will be more environmentally friendly, as the active component will be desorbed in small but effective concentrations. Furthermore, during the synthesis process, the toxic template (CTAB) will be replaced with biogenic templates based on bacterial cells. 2. Experimental part 2.1 Reagents Silica sol − gel matrix based on methyltriethoxysilane (MTES) and tetraethoxysilane (TEOS) (Sigma − Aldrich, USA), and 5% aqueous PVA solution (FerakBerlin, Germany) was used as the basis for formation porous carrier with bacterial cells. Cetyltrimethylammonium bromide (CTAB) (Macklin, China) was used as a traditional surfactant template. Octenidine dihydrochloride (OCT) (Macklin, China) was used as the active antimicrobial agent. 2.2 Microorganism cultivation Bacterial strains of the Rhodococcus fascians VKM B-1462 and Pseudomonas veronii VKM B-877 were obtained from the All-Russian collection of microorganisms (VKM) of the Institute of Biochemistry and Physiology of Microorganisms, Pushchino. Cultivation of the Rhodococcus fascians VKM B-1462 was carried out on a Corynebacterium agar (Casein peptone 10.0 g; Yeast extract 5.0 g; Glucose 5.0 g; NaCl 5.0 g; Agar 15.0 g (Helicon, Russia)). Cultivation of the Pseudomonas veronii VKM B-877 was carried out on a Peptone meat agar (Peptone 10.0 g; NaCl 5.0 g; Beef extract 3.0 g; Agar 20.0 g (Helicon, Russia)). Cultures of microorganisms were grown in flasks with a volume of 750 cm 3 (medium volume 200 cm 3 ) at 28°C and aeration in a shaker at 180 rpm. The inoculum was introduced in an amount of 2% by volume of the medium to a final concentration of ~ 10 8 CFU/cm 3 . The grown biomass was centrifuged at 10000 rpm for 10 min, and the precipitate was washed with phosphate buffer solution (20 mmol/dm 3 , pH 6.8). Cell biomass was stored in microtubes at − 15°C. 2.3 Formation of organosilicon biohybrid material Rhodococcus fascians VKM B-1462 and Pseudomonas veronii VKM B-877 bacteria strains were used as biotemplates to obtain materials of types I and II, respectively. To synthesize the silicon material, 172 µl of a 5% polyvinyl alcohol solution was added to a 430 µl cell suspension (1.0 ± 0.1 × 10⁹ CFU/ml) in a phosphate buffer solution (pH 6.8), which was then mixed for five minutes using an Elmi CM-70M07 mixer (Riga, Latvia). Then, add a mixture of methyltriethoxysilane and tetraethoxysilane in volume ratios of 50/50 to the mixture. Stir the mixture at 100 rpm for 5 minutes. Then added 43 µl basic catalysts 0.2 M NaF and stir at 100 rpm for 15 minutes. Leave to dry in air at room temperature for 24 hours. The synthesis of materials of types III and IV was achieved by the addition of 170 mg of cetyltrimethylammonium bromide (CTAB) and 50 mg of NaOH (Helicon, Russia) to 80 ml of distilled water at 80°C. The mixture was stirred until complete dissolution occurred. Subsequently, 417 µl of methyltriethoxysilane and 417 µl of tetraethoxysilane (for type III) or 834 µl of tetraethoxysilane (for type IV) were added dropwise and stirred for 2 hours. Thereafter, the mixture was precipitated in a centrifuge at a speed of 10,000 rpm for 5 minutes. The specimens were then subjected to a thorough cleansing process involving distilled water, followed by drying in an oven at a precise temperature of 90°C. 2.4 Removal of templates from the formed material Materials of types I–IV, formed using templates, were then annealed in an oxygen atmosphere at a heating rate of 10°C per minute at temperatures of 200, 400, 600, 800, 1000 and 1200°C, with a 1-hour hold at the set temperature. 2.5 Scanning electron microscopy (SEM) Prior to measurements, samples encapsulated in a silica sol-gel matrix of Rhodococcus fascians and Pseudomonas veronii cells were mounted on a 25 mm diameter aluminum pin, fixed with conductive carbon adhesive tape and covered with a thin film (15 nm) of carbon. Observations were conducted using a Hitachi TM4000Plus scanning electron microscope. Images were acquired in secondary electron mode at an accelerating voltage of 10 kV. EDX-SEM studies were performed using an Oxford Instruments X-max 80 EDX system at an accelerating voltage of 10 kV. 2.6 IR spectroscopy IR spectra of materials were recorded on a Fourier infrared spectrometer FMS 1201 (Monitoring, St. Petersburg, Russia). The samples and pure potassium bromide were dried for 2 hours in a desiccator at 90 degrees Celsius. The spectra of the solids were measured using KBr discs. A solid sample (3 mg) was thoroughly mixed with potassium bromide (150–200 mg) in a mortar and the mixture was pressed at a pressure of 10 t/cm² for 3 min. The resulting sample spectrum was recorded relative to the background air spectrum. 2.7 Sorption of octenidine dihydrochloride In order to study the desorption properties of the formed materials, 20 milligrams of the sample was placed in 4 milliliters of octenidine dihydrochloride solution (titer − 5 mg/ml) for a different period of time at 180 rpm. Subsequently, the materials were separated by centrifugation (10,000 rpm, 5 min). The upper layer was then decanted and analyzed, after which the material was dried at 85°C for a period of two hours. 2.8 Study of desorption capacity At 30-minute intervals, 5 mg of the substance was deposited into a test tube, followed by the addition of 30 ml of distilled water. The mixture was then subjected to a centrifugal process at 10,000 rpm for a duration of 10 minutes. Two milliliters of the solution were placed into a quartz cuvette, after which the optical density was measured at a wavelength of 280 nm. 3. Results and Discussions The following four categories of material were formed. The formation of materials of types I and II involved the use of bacterial cells, specifically Rhodococcus fascians VKM B-1462 and Pseudomonas veronii VKM B-877, as biotemplates (Fig. 1 ). These two strains of microorganisms display significant disparities in morphology, a factor that has the potential to influence the materials obtained and their sorption capacity. The material employed for the formation of the silica shells was composed of methyltriethoxysilane and tetraethoxysilane in a volume ratio of 50/50 vol%, a proportion that had been determined to be more effective for complete encapsulation of bacteria [ 31 ]. As comparison samples, type III and IV materials were synthesized using cetyltrimethylammonium bromide micelles as templates, using methyltriethoxysilane and tetraethoxysilane 50/50 vol.%, as well as 100 vol.% TEOS. The compositions under scrutiny were selected to elucidate the discrepancy between the utilization of biotemplates and CTAB, as well as a conventional material based on tetraethoxysilane in combination with CTAB. 3.1 Determination of the morphology of silicon-containing materials obtained using various templates In the initial phase of the research, a comprehensive array of materials was subjected to a thermal treatment within the temperature range of 200–1200°C. It is evident that all samples under consideration are fine-grained materials. The matrices that were not subjected to heat treatment are white in color. It has been established that when the materials are subjected to annealing at temperatures of 200 and 400°C, they exhibit a brown coloration. However, at a temperature of 600°C, a transformation to a light grey hue is observed. Samples of types I and II exhibit white coloration at temperatures in excess of 800°C. Materials of types III and IV, when subjected to temperatures ranging from 800 to 1200°C, exhibit a light grey hue accompanied by dark grey spots. This phenomenon may be attributed to the incomplete annealing of cetyltrimethyl ammonium bromide (CTAB) micelles. This suggests the presence of residual carbon within the materials, as illustrated in Fig. 2 . The morphology of the materials is influenced by the type of template used. Type I samples that have not undergone annealing typically exhibit a plate-like appearance; however, upon reaching 800°C, they typically acquire a powdery appearance. Type II materials consist of thicker (up to 2 mm) platelets that retain their structure even after annealing at 1200°C. This phenomenon may be attributed to variations in the composition of the cell wall of gram-positive and gram-negative bacteria, including the number of hydroxyl groups present. Materials obtained using CTAB micelles exhibit no change in appearance, forming fine powders. In the subsequent stage of the research, the morphology of the obtained materials was studied using scanning electron microscopy in order to investigate the influence of templates and annealing temperature (Fig. 3 ). Materials formed using microorganism cells consist of particles remaining after encapsulation of bacteria. Rhodococcus fascians cells were used for sample type I, and when immobilized, silicon-containing shells with average dimensions of 1×1.6 µm are formed. In the process of forming type II material using Pseudomonas veronii cells, the formation of more elongated cylindrical particles with dimensions of 1×3.5 µm is observed, which corresponds to the description of their morphology. It has been established that at annealing temperatures of 200 and 400°C, rough materials exhibit uneven areas of irregular shape. At a temperature of 600°C, materials of types I and II are characterized by a smoother folded layer with cylindrical particles. Prior to the initiation of the melting process, the dimensions of the cylindrical particles remain constant. Type III material is formed from small spherical particles that are joined together, and this morphology is observed at annealing temperatures ranging from 200 to 600°C. Type IV material is more uniform than type III material. This phenomenon may be attributed to the use of a single silane precursor, tetraethoxysilane, which facilitates both hydrolysis and polycondensation reactions across all four Si-O bonds. At the same time, the introduction of a second silane precursor, methyltriethoxysilane, into the type III material allows for a more heterogeneous surface to be obtained, which may have a more developed surface area, and consequently affect the sorption properties. All materials are characterized by matrix fusion at annealing temperatures ranging from 800 to 1200°C. It has been demonstrated that at 800°C, materials formed using bacterial cells become more porous, and cylindrical particles on the surface disappear. It is evident that an increase in temperature to 1200°C results in a further enhancement of the samples' smoothness. The type I matrix sample is a smooth material with round visible cavities. Materials of types II and III are characterized by their heterogeneity, with pores of varying sizes. At an elevated temperature of 1200°C, the type IV material exhibits a monolithic structure, devoid of any discernible pores on the surface. The morphology of materials obtained by employing different templates can exhibit notable variation, which can subsequently result in divergent sorption and desorption properties when utilized as loading platforms. 3.2 Confirmation of silicon carrier formation using IR spectroscopy In order to confirm the formation of polymeric silicon-containing materials, their samples were studied using IR spectroscopy (Fig. 4 ). The materials are characterized by peaks that are indicative of the formation of Si-O-Si bonds. The 800 cm -1 spectrum is indicative of inter-tetrahedral vibrations, while the 1075 and 1100 cm -1 peaks correspond to stretching vibrations. Despite annealing, all materials demonstrate an retention of CH 3 groups, being part of methyltriethoxysilane, which exhibit stretching vibrations at 1265 (1285) cm − 1 and bending vibrations at 1400 cm − 1 . However, it is observed that the intensity of these vibrations decreases with increasing temperature. The presence of bonds at 1650 cm − 1 is indicative of the occurrence of bending vibrations of OH groups. Furthermore, for materials of types III and IV, at processing temperatures up to 600°C, peaks with high intensity at 2850 and 2965 cm − 1 are evident. These vibrations may be indicative of asymmetric and symmetric stretching vibrations of CTAB. [ 43 ]. Furthermore, at temperatures up to 400°C, asymmetric stretching vibrations of N + (CH 3 ) 3 groups and insignificant vibrations of bromide anions at 720 cm − 1 are observed, which suggests that up to 600°C, cetyltrimethylammonium bromide remains in the material, as described in the literature, which may have a negative effect on the loading capacity of the matrices [ 28 , 44 ]. 3.3 Study of the sorption and desorption properties of materials Octenidine dihydrochloride, a quaternary ammonium compound with proven antiseptic efficacy, was applied to the obtained material samples [ 42 , 45 ]. To determine the sorption time, materials of types I and II were used, which showed the best loading capacity in preliminary studies (Fig. 5 ). The dependence of octenidine sorption in materials on the exposure time of samples in a concentrated solution was determined using UV spectroscopy. It has been determined that the optimal time for sorption of these materials is 1 hour, after which octenidine desorbs from the surface of the samples into the solution. Subsequent studies were conducted with sorption for a duration of one hour. Furthermore, the desorption properties of the materials were the subject of study. The results of the study are presented in Table 1 . Table 1 Characteristics of the formed materials Matrix OСT loading, µg/1 mg of carrier Cumulative release of OCT from 1 mg of carrier, µg 200 °С I type 183 16 II type 146 12 III type 199 27 IV type 213 27 400 °С I type 217 29 II type 229 32 III type 269 33 IV type 253 31 600 °С I type 303 87 II type 268 53 III type 299 63 IV type 311 67 800 °С I type 376 130 II type 340 94 III type 318 73 IV type 327 69 1000 °С I type 271 56 II type 253 46 III type 176 28 IV type 183 30 1200 °С I type 277 18 II type 321 23 III type 294 19 IV type 302 18 For silicon-containing matrices of all types, an increase in sorption and desorption characteristics is observed with rising temperature. The highest values for octenidine loading and release are achieved for materials with an annealing temperature of 800°C. This can be attributed to changes in the morphology of the samples upon reaching the Tammann temperature for silicates (759°C). An further increase in temperature has been shown to result in the fusion of the matrices, which has a negative effect on their characteristics. Materials obtained using biotemplates have been shown to exhibit high values of adsorbed and desorbed octenidine. The highest values of octenidine sorption and desorption are observed in type I matrix formed using Rh. fascians cells (Fig. 6 ) and annealing temperature of 800°C. This phenomenon may be attributed to the formation of material with sufficiently large transport pores, thereby enabling octenidine to desorb without diffusion difficulties. The kinetic curves for octenidine release were plotted for materials of all types with different annealing temperatures (Fig. 7 ). The release profiles for materials that have undergone annealing at 800°C are distinguished by the presence of two distinct stages. The initial stage is characterized by a short-term release effect, while the subsequent stage is marked by a more prolonged release, exhibiting an approximate linear dependence. For types I, III, IV samples at 600°C, a similar release pattern is observed, but with lower cumulative release values. Materials formed using CTAB are characterised by the release of smaller amounts of octenidine, which may be due to the morphology and preservation of larger amounts of carbon after annealing. The size of CTAB micelles is 2–3 nm, which is comparable to the size of the octenidine molecule (0.9 nm). This has the effect of causing diffusion difficulties during the desorption of the active substance. Kinetic models (zero order, first order, Korsmeyer-Peppas) were utilised to characterise the release of octenidine from all materials at an annealing temperature of 800°C (Table 2 ). The data processing using a modified Korsmeyer-Peppas model that takes into account the burst effect is characterised by high correlation coefficients. For all materials in the first stage with a burst effect, the diffusion exponents n I of the Korsmeyer-Peppas model range from 0.37 to 0.57, which is characteristic of Fickian diffusion-controlled release. The release constants k I in the first stage exceed the release constants in the second stage to a significant degree, thus confirming two-stage desorption. In stage I, more than 20% of octenidine is rapidly released from the surface of materials or from large transport pores. The diffusion flow is directed towards the concentration gradient, and diffusion difficulties are minimal. Type I material is characterised by the highest constant. In the second stage, the diffusion exponent values exceed 0.43 for all materials, indicating abnormal transport release occurring according to non-Fickian diffusion. Various diffusion difficulties arise due to the release of octenidine from the thickness of the materials, the desorption rate decreases, and re-adsorption of octenidine on the surface is possible due to the presence of OH groups in the matrices, as well as the occurrence of steric difficulties due to the peculiarities of the morphology of the materials. The values of the constants are significantly lower than in stage I of release. Despite the use of different ratios of silane precursors, there is a insignificant difference in the sorption and desorption properties of materials of types III and IV. This may indicate that the nature of the template has a great influence. The utilisation of Rhodococcus fascians VKM B-1462 and Pseudomonas veronii VKM B-877 bacterial cells as biotemplates resulted in a 1.3–1.7 fold increase in the desorption of octenidine compared to materials formed using cetyl trimethyl ammonium bromide (130 and 94 µg of octenidine/mg of carrier for bacterial templates, 73 and 69 µg of octenidine/mg of carrier for surfactants).Consequently, bacterial cells can be utilized as a viable alternative to conventional templates. The employment of these biotemplates has the potential to facilitate the formation of porous materials for sorption, drug delivery systems, catalyst carriers, and antibacterial materials 4. Conclusion The application of microbial cells as biological templates for the synthesis of silica has been shown to be an effective method for the controlled release of octenidine. The optimal heat treatment was determined to be annealing at 800°C, a process which ensured the removal of the biological template without sintering the silicate matrix. At lower temperatures, templates persist within the samples, thereby impacting the loading of octenidine. It is evident that an increase in temperature results in material fusion. This phenomenon has a subsequent impact on the pore volume, which is reduced, and the sorption capacity of the matrices, which is negatively affected. The optimal time for maximum adsorption of octenidine (18%) was determined to be 1 hour, after which the substance desorbs into the solution. An increase in adsorbed octenidine is observed when the annealing temperature of the materials is raised to 800°C, after which there is a decrease in loading capacity, a characteristic of all samples. The highest adsorption was observed for the matrix obtained using Gram-positive bacteria Rhodococcus fascians VKM Ac-1462. A comparative analysis demonstrated the advantage of the Gram-positive bacterium Rhodococcus fascians VKM B-1462 over the Gram-negative Pseudomonas veronii VKM B-877 when used as biotemplates. The rigid cell wall of R. fascians , stabilized by mycolic acids, retained its structural integrity during the ageing and drying of the sol-gel material. This ensured the formation of a porous surface with higher sorption characteristics. In contrast, the thin cell wall of P. veronii was likely to undergo rapid deformation during xerogel formation. The elongated shape of P. veronii also contributes to the sorption properties of the material, leading to the formation of an irregular pore structure with defects. In the process of annealing materials formed using CTAB, the quaternary ammonium compound is known to remain in the matrices. This phenomenon has been confirmed through the analysis of infrared spectroscopy data. The presence of CTAB in samples III and IV has been shown to affect the sorption and desorption of octenidine. This, in turn, requires a change in the procedure for removing surfactants from silicate material in order to improve the properties of the materials. The kinetic curves obtained demonstrate that matrices with an annealing temperature of 800°C are characterised by two-phase desorption, in which the initial stage involves the explosive release of the active substance from the surface of the samples, followed by a phase of prolonged release from the depths of the material. It was determined that the desorption of octenidine in the initial phase is in accordance with Fick's diffusion theory. The mechanism of desorption in the second stage is non-Fickian diffusion; the release of octenidine is slowed down due to diffusion difficulties caused by the possible re-adsorption of octenidine in the narrow pores of the matrices. In comparison with the CTAB template, the employment of microbial cells has the potential to offer a number of applications due to cost-effectiveness, environmental safety, and ease of removal of the pore-forming agent. The results confirm the hypothesis that biological templates can be utilized to create functional porous materials for the controlled delivery of antiseptic agents and drugs. Subsequent research will concentrate on the study of the antimicrobial activity of the obtained materials, the testing of biocompatibility, and the investigation of the possibility of adding alternative antiseptics and medicinal products. Declarations Conflict of Interest The authors declare no competing financial interest. Funding This research was funded by grants from the Russian Science Foundation, RSF № 25-23-00410, https://rscf.ru/en/project/25-23-00410/ . Author Contribution All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by EAL, PVA and KDA. The first draft of the manuscript was written by EAL and OAK, all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. References Rashid AB, Shishir SI, Mahfuz MA, Hossain MT, Hoque ME (2023) Silica aerogel: synthesis, characterization, applications, and recent advancement. Part Part Syst Charact. https://doi.org/10.1002/ppsc.202200186 Nayl AA, Abd-Elhamid AI, Aly AA, Bräse S (2022) Recent progress in the applications of silica-based nanoparticles. RSC Adv. https://doi.org/10.1039/D2RA01587K Ciriminna R, Pagliaro M (2022) Open challenges in sol–gel science and technology. J Sol-Gel Sci Technol. https://doi.org/10.1007/s10971-021-05535-9 Yu X, Williams CT (2022) Recent advances in the applications of mesoporous silica in heterogeneous catalysis. Catal Sci Technol. https://doi.org/10.1039/D2CY00001F Shinde PS, Suryawanshi PS, Patil KK, Belekar VM, Sankpal SA, Delekar SD, Jadhav SA (2021) A brief overview of recent progress in porous silica as catalyst supports. J Compos Sci. https://doi.org/10.3390/jcs5030075 Wang J, Liu Y, Cai Q, Dong A, Yang D, Zhao D (2022) Hierarchically porous silica membrane as separator for high-performance lithium‐ion batteries. Adv Mater. https://doi.org/10.1002/adma.202107957 Oh JY, Yang G, Choi E, Ryu JH (2022) Mesoporous silica nanoparticle-supported nanocarriers with enhanced drug loading, encapsulation stability, and targeting efficiency. Biomater Sci. https://doi.org/10.1039/D2BM00010E Emrie DB (2024) Sol–gel synthesis of nanostructured mesoporous silica powder and thin films. J Nanomater. https://doi.org/10.1155/2024/6109770 Lionello DF, Ramallo JI, Soler-Illia GJ, Fuertes MC (2022) Mechanical properties of ordered mesoporous oxides thin films. J Sol-Gel Sci Technol. https://doi.org/10.1007/s10971-021-05626-7 Kurt T, Topuz B (2021) Sol-gel control on mixed network silica membranes for gas separation. Sep Purif Technol. https://doi.org/10.1016/j.seppur.2020.117654 Kamanina OA, Lantsova EA, Rybochkin PV, Arlyapov VA, Plekhanova YV, Reshetilov AN (2022) The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices. Membranes. https://doi.org/10.3390/membranes12100983 Fotie J (2025) Applications of tailored mesoporous silicate nanomaterials in regenerative medicine and theranostics /. Int J Mol Sci. https://doi.org/10.3390/ijms26167918 Wang G, Xu X, Cheng Q, Hu J, Xu X, Zhang Y et al (2023) Preparation of sustainable release mesoporous silica nano-pesticide for control of Monochamus alternatus. Sustain Mater Technol. https://doi.org/10.1016/j.susmat.2022.e00538 Fan X, Wu J, Zhang T, Liu J (2024) Electrochemical/electrochemiluminescence sensors based on vertically-ordered mesoporous silica films for biomedical analytical applications. ChemBioChem. https://doi.org/10.1002/cbic.202400320 Kamanina OA, Lantsova EA, Rybochkin PV (2025) Efficient Approach to the Immobilization of Glucose Oxidase in Sol-Gel Polymeric Matrices Using Silane Precursors with One and Two Non-Hydrolyzable Bonds J. Anal Chem. https://doi.org/10.1134/S1061934825700583 Ekeoma BC, Yusuf M, Johari K, Abdullah B (2022) Mesoporous silica supported Ni-based catalysts for methane dry reforming: A review of recent studies. Int J Hydrogen Energy. https://doi.org/10.1016/j.ijhydene.2022.05.297 Dong W, Yan J, Yang Y, Wu Q, Hu X (2024) Immobilization of laccase on magnetic mesoporous silica as a recoverable biocatalyst for the efficient degradation of benzo [a] pyrene // Chemosphere. https://doi.org/10.1016/j.chemosphere.2023.140642 Druzian DM, Da Silva MDC, Da Silva WL, Santos JHZD (2025) Sol-Gel Technologies for Drug Delivery in Potential Cancer Treatments. Novel Approaches in Cancer Treatment: Tumor Targeted Therapy. https://doi.org/10.1007/978-3-032-00771-1_397 Zhu H, Zheng K, Boccaccini AR (2021) Multi-functional silica-based mesoporous materials for simultaneous delivery of biologically active ions and therapeutic biomolecules. Acta Biomater. https://doi.org/10.1016/j.actbio.2021.05.007 Pellicer-Castell E, Belenguer-Sapiña C, Amoros P, El Haskouri J, Herrero-Martínez JM, Mauri-Aucejo AR (2022) Mesoporous silica sorbent with gold nanoparticles for solid-phase extraction of organochlorine pesticides in water samples. J Chromatogr A. https://doi.org/10.1016/j.chroma.2021.462729 Ripon RI, Begum ZA, Miyazawa R, Leonard K, Ogata M, Ahmmad B, Rahman IM (2024) Development and application of a new macrocyclic ligand-functionalized mesoporous silica sorbent for selective separation of radiocesium from environmental wastewater. Microchem J. https://doi.org/10.1016/j.microc.2024.111649 Namdar N, Nayeri Fasaei B, Shariati P, Joghataei SM, Arpanaei A (2024) Mesoporous silica nanoparticles co-loaded with lysozyme and vancomycin for synergistic antimicrobial action. Sci Rep Nat. https://doi.org/10.1038/s41598-024-78922-1 Alharthi S, Ziora ZM, Janjua T, Popat A, Moyle PM (2022) Formulation and Biological Evaluation of Mesoporous Silica Nanoparticles Loaded with Combinations of Sortase A Inhibitors and Antimicrobial Peptides. Pharmaceutics. https://doi.org/10.3390/pharmaceutics14050986 Lantsova EA, Pertseva VA, Soromotin VN, Saverina EA, Kamanina OA (2025) Functionalized Organosilicon Material as a Potential Support for the Loading of Active Compounds. Silicon. https://doi.org/10.1007/s12633-025-03293-9 Barman S, Fager C, Röding M et al (2021) New Characterization Measures of Pore Shape and Connectivity Applied to Coatings used for Controlled Drug Release. J Pharm Sci. https://doi.org/10.1016/j.xphs.2021.02.0242021 Beltrán-Aburto R, Borjas-García SE, Martínez-Torres P, Villegas J, Medina Flores A (2025) Synthesis of Mesoporous Samarium Oxide by Using CTAB. Microsc Microanal. https://doi.org/10.1093/mam/ozaf048.1054 De Araujo BRS, Onrubia-Calvo JA, Stambouli I et al (2022) Towards the development of advanced hierarchical chabazite materials: Novel micro-mesoporous silicoaluminophosphate SAPO-34 zeolites. Mater Today Commun. https://doi.org/10.1016/j.mtcomm.2022.103580 Carvalho GC, Marena GD, Karnopp JCF et al (2022) Cetyltrimethylammonium bromide in the synthesis of mesoporous silica nanoparticles: General aspects and in vitro toxicity. Adv Colloid Interface Sci. https://doi.org/10.1016/j.cis.2022.102746 Srujana TL, Rao KJ, Korumilli T (2025) Natural Biogenic Templates for Nanomaterial Synthesis: Advances, Applications, and Environmental Perspectives. ACS Biomater Sci Eng. https://doi.org/10.1021/acsbiomaterials.4c02075 Oh D, Ozgit-Akgun C, Akca E et al (2017) Biotemplating pores with size and shape diversity for Li-oxygen Battery Cathodes. Sci Rep. https://doi.org/10.1038/srep45919 Rybochkin PV, Kamanina OA, Lantsova EA, Arlyapov VA, Saverina EA (2023) Characterization of the catalytic ability and surface properties of a heterogeneous biocatalyst obtained by the sol-gel method. J Sol-Gel Sci Technol. https://doi.org/10.1007/s10971-022-05915-9 Kamanina OA, Lantsova EA, Rybochkin PV et al (2023) 3-in-1 Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution. ACS Appl Mater Interfaces. https://doi.org/10.1021/acsami.3c09897 Lantsova EA, Kamanina OA, Rybochkin PV, Arlyapov VA, Saverina EA, Vereshchagin AN (2024) Immobilization of microbial cells in a sol-gel matrix using a precursor with two non-hydrolyzed bonds. J Sol-Gel Sci Technol. https://doi.org/10.1007/s10971-023-06275-8 Zhang X, Li S, Deng Y, Zuo Z, Sun Z, Li C, Zheng S (2023) Benzalkonium chloride modified kaolinite with rapid bactericidal activity for the development of antibacterial films. Mater Today Commun. https://doi.org/10.1016/j.mtcomm.2023.106152 Khelissa S, Gharsallaoui A, Fadel A et al (2021) Microencapsulation of benzalkonium chloride enhanced its antibacterial and antibiofilm activities against Listeria monocytogenes and Escherichia coli. J Appl Microbiol. https://doi.org/10.1111/jam.15010 Khan S, Amin F, Amin R, Kumar N (2024) Exploring the Effect of Cetylpyridinium Chloride Addition on the Antibacterial Activity and Surface Hardness of Resin-Based Dental Composites. Polymers. https://doi.org/10.3390/polym16050588 Cabral-Romero C, Hernández-Delgadillo R, Nakagoshi-Cepeda SE et al (2024) Antimicrobial and antitumor activities of an alginate-based membrane loaded with bismuth nanoparticles and cetylpyridinium chloride. J Appl Biomater Funct Mater. https://doi.org/10.1177/22808000241236590 Kim M, Weigand MR, Oh S et al (2018) Widely Used Benzalkonium Chloride Disinfectants Can Promote Antibiotic Resistance. Appl Environ Microbiol. https://doi.org/10.1128/AEM.01201-18 Mao X, Auer DL, Buchalla W et al (2020) Cetylpyridinium Chloride: Mechanism of Action, Antimicrobial Efficacy in Biofilms, and Potential Risks of Resistance. Antimicrob Agents Chemother. https://doi.org/10.1128/aac.00576-20 Kampf G (2024) Benzalkonium Chloride BT - Antiseptic Stewardship: Biocide Resistance and Clinical Implications / ed. Kampf G. Springer International Publishing, Cham Koeck R, Denkel L, Fessler AT et al (2023) Clinical Evidence for the Use of Octenidine Dihydrochloride to Prevent Healthcare-Associated Infections and Decrease Staphylococcus aureus Carriage or Transmission—A. Rev Pathogens. https://doi.org/10.3390/pathogens12040612 Rzycki M, Drabik D, Szostak-Paluch K et al (2021) Unraveling the mechanism of octenidine and chlorhexidine on membranes: Does electrostatics matter? Biophys. J. https://doi.org/10.1016/j.bpj.2021.06.027 Ai F, Zhao G, Lv W, Lin J (2020) Facile synthesis of cetyltrimethylammonium bromide-loaded mesoporous silica nanoparticles for efficient inhibition of hepatocellular carcinoma cell proliferation. Mater Res Express. https://doi.org/10.1088/2053-1591/abad15 Han HW, Joe A, Jang ES (2021) Reduced cytotoxicity of CTAB-templated silica layer on gold nanorod using fluorescence dyes and its application in cancer theranostics. J Ind Eng Chem. https://doi.org/10.1016/j.jiec.2021.01.020 Malanovic N, Buttress JA, Vejzovic D et al (2022) Disruption of the Cytoplasmic Membrane Structure and Barrier Function Underlies the Potent Antiseptic Activity of Octenidine in Gram-Positive Bacteria. Appl Environ Microbiol. https://doi.org/10.1128/aem.00180-22 Additional Declarations No competing interests reported. Supplementary Files GraphicalAbstract.png Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 15 May, 2026 Reviews received at journal 19 Jan, 2026 Reviewers agreed at journal 13 Jan, 2026 Reviewers invited by journal 07 Jan, 2026 Editor assigned by journal 11 Dec, 2025 Submission checks completed at journal 11 Dec, 2025 First submitted to journal 08 Dec, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8310871","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":571711225,"identity":"672fe95c-2bca-4ccb-b965-55777b42763c","order_by":0,"name":"Elizaveta A. Lantsova","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABEElEQVRIie2PsUrEQBCGRwJnc7KdTBDZV9jjmisOfJVZDlJfaSHhRLg06rURH8EmEki9sJAqwTaiRSo7YStJJW4UDos1h53gfsXsDMzH/Avg8fxV2u8D6wstdygEgNsuXPWv+I0iVN8MKAyCFujsOebJ5cTsdbHMH+6kaQVwdqicSrgaCaDyBUVVTRFIy6J5zdEGm9zcklOxMawy0igwIqsoq9RZr5B4cisnat8AvWvkabTogGywtM67IUXAWIBca4RmUdorgczYVTF4BfV4qeS1DrNKlzOK9DRtDooZCfzxLyxJ7lvzphlPzi8aM4+PN5s6f+xO55wduRUIANR2+NzBr+ped8PUzhWPx+P5X3wAOwdgffRr/1AAAAAASUVORK5CYII=","orcid":"","institution":"Tula State University","correspondingAuthor":true,"prefix":"","firstName":"Elizaveta","middleName":"A.","lastName":"Lantsova","suffix":""},{"id":571711226,"identity":"4fcae5e2-077b-454a-b394-702b0c079888","order_by":1,"name":"Violetta A. Pertseva","email":"","orcid":"","institution":"Tula State University","correspondingAuthor":false,"prefix":"","firstName":"Violetta","middleName":"A.","lastName":"Pertseva","suffix":""},{"id":571711227,"identity":"1d399c8f-797d-41e4-b88f-0530a3835484","order_by":2,"name":"Denis A. Kolykhalov","email":"","orcid":"","institution":"Tula State University","correspondingAuthor":false,"prefix":"","firstName":"Denis","middleName":"A.","lastName":"Kolykhalov","suffix":""},{"id":571711228,"identity":"cec35609-9818-4b73-b13d-2506f772b870","order_by":3,"name":"Olga A. Kamanina","email":"","orcid":"","institution":"Tula State University","correspondingAuthor":false,"prefix":"","firstName":"Olga","middleName":"A.","lastName":"Kamanina","suffix":""}],"badges":[],"createdAt":"2025-12-08 20:23:14","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8310871/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8310871/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":100363887,"identity":"d207f140-b82e-41c5-a154-021e7284d93b","added_by":"auto","created_at":"2026-01-16 07:51:59","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":3738741,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.docx","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/cfa35669a742ed1e0eaccf86.docx"},{"id":100363716,"identity":"8c3cc330-994b-4dc8-9ea0-c466b3dc29cc","added_by":"auto","created_at":"2026-01-16 07:51:20","extension":"json","order_by":1,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":5686,"visible":true,"origin":"","legend":"","description":"","filename":"71c1d4b90902405bb0b966c65b9dcd1b.json","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/8e15d9fa0c367e490e2ebd4d.json"},{"id":100041811,"identity":"188f9cf8-5eb1-4590-9bab-c8674e5c9b29","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"xml","order_by":2,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":128769,"visible":true,"origin":"","legend":"","description":"","filename":"71c1d4b90902405bb0b966c65b9dcd1b1enriched.xml","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/fcbd372a005a08fa0b1367f4.xml"},{"id":100363276,"identity":"6a2a55e1-b40a-4ff7-b9ef-399caa16731c","added_by":"auto","created_at":"2026-01-16 07:49:16","extension":"png","order_by":3,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":473042,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/5e72fc86d95c5fc65f469cae.png"},{"id":100041804,"identity":"7f5c9f58-8e33-47b8-ad20-a2b68342390e","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"jpeg","order_by":4,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":323079,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage2.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/34e72e8d7138e1e65f9e360b.jpeg"},{"id":100041818,"identity":"b0395d62-ff09-46a2-b39e-ab443ed2485c","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"jpeg","order_by":5,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":399835,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/3376d1047d201003e8d643d2.jpeg"},{"id":100363833,"identity":"d4c6e483-f57b-4025-8bda-4b5288913843","added_by":"auto","created_at":"2026-01-16 07:51:54","extension":"jpeg","order_by":6,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":2065758,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/7adc4bba29334fa9ad688f3c.jpeg"},{"id":100363915,"identity":"b2f40c74-c9ff-47ed-8a47-44a204121367","added_by":"auto","created_at":"2026-01-16 07:52:04","extension":"jpeg","order_by":7,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1023086,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/8cbc16d930ec2fd535c06a70.jpeg"},{"id":100362844,"identity":"a4fd0776-f1f3-4e20-b0f4-5cdaec8541a4","added_by":"auto","created_at":"2026-01-16 07:48:09","extension":"jpeg","order_by":8,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1129354,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage6.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/267ebdd8faa4c022a3e4605d.jpeg"},{"id":100041814,"identity":"0e00b2f3-4ec6-4511-be0f-060560f7348d","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":9,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":169037,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage7.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/12ae0faa2a4777df172286e1.png"},{"id":100363895,"identity":"e0c1998f-f21d-43ee-be1e-054fb93802e7","added_by":"auto","created_at":"2026-01-16 07:51:59","extension":"jpeg","order_by":10,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":533190,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage8.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/0af4c91a64537d2190de2b29.jpeg"},{"id":100363881,"identity":"9c8402be-67ca-4924-874d-f628c93e1bf9","added_by":"auto","created_at":"2026-01-16 07:51:59","extension":"jpeg","order_by":11,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":324503,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage9.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/54391972e056cb99c129b626.jpeg"},{"id":100041823,"identity":"f58d0d9e-76a5-47ad-8172-29f54e16b4bc","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":12,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":59017,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/179a30408ff6a0bb6939848e.png"},{"id":100363830,"identity":"25bd57b8-4bc1-4cab-b9e9-07053668b6ab","added_by":"auto","created_at":"2026-01-16 07:51:53","extension":"png","order_by":13,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":181167,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/827daf5f4f8537c3e60244d8.png"},{"id":100362845,"identity":"611622f4-5fa7-4128-9ece-52208a73ffe6","added_by":"auto","created_at":"2026-01-16 07:48:09","extension":"png","order_by":14,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":179311,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/56599bb05589cf3a640e64dc.png"},{"id":100363347,"identity":"94af8a6b-0ed8-4b5b-b2f5-08d8cb3557c3","added_by":"auto","created_at":"2026-01-16 07:49:31","extension":"png","order_by":15,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":720991,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage4.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/5dd9a2d1ed63c4c7de781f2e.png"},{"id":100363772,"identity":"a6f7f29a-eb58-4bda-96ff-95fa36248574","added_by":"auto","created_at":"2026-01-16 07:51:44","extension":"png","order_by":16,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":218026,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage5.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/e6c75aba5689594fee51c45d.png"},{"id":100041821,"identity":"3ddc30f7-7259-4fea-b2c0-d4c78de41677","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":17,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":238086,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage6.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/7dd79d1456f5f6d30bf34c9f.png"},{"id":100041830,"identity":"36176934-1a1a-4d2d-aafc-25e41fe6f304","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":18,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":56535,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage7.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/6460fb27f0af3cd5af5fc272.png"},{"id":100363283,"identity":"6202f7e1-1693-4e4f-957e-5ce443cc0feb","added_by":"auto","created_at":"2026-01-16 07:49:16","extension":"png","order_by":19,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":120679,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage8.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/99ea03d83c412cad4a19ffb9.png"},{"id":100041826,"identity":"99392442-84ba-4f11-a480-c63a91bda057","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":20,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":83076,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage9.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/af4a66b2db58d919fdf09017.png"},{"id":100041832,"identity":"129850e7-ab86-4d64-85cb-5133b3489c8a","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"xml","order_by":21,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":127267,"visible":true,"origin":"","legend":"","description":"","filename":"71c1d4b90902405bb0b966c65b9dcd1b1structuring.xml","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/32fd3d02da9f4287961992dc.xml"},{"id":100363618,"identity":"7baf4ea2-5c64-4954-86d6-f59302592457","added_by":"auto","created_at":"2026-01-16 07:50:46","extension":"html","order_by":22,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":142377,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/abf39a42d85e3b5ff5eb06bc.html"},{"id":100041803,"identity":"8f9a9c62-4c41-4818-8017-1c345a1b0ae5","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":393037,"visible":true,"origin":"","legend":"\u003cp\u003eScanning electron microscopy: A – culture of \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 microorganisms; B – culture of \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877 microorganisms.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/b067491246c050ba0e776cea.png"},{"id":100041807,"identity":"f3f01f16-cf9a-4ac9-850b-555d096725b2","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":865925,"visible":true,"origin":"","legend":"\u003cp\u003eExternal appearance of formed matrices of types I-IV\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/483fe30938fa44823b3b7a35.png"},{"id":100363597,"identity":"58fcffef-f5ae-454d-ac57-4b2eafc9bb2a","added_by":"auto","created_at":"2026-01-16 07:50:35","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":294244,"visible":true,"origin":"","legend":"\u003cp\u003eScanning electron microscopy for type I-IV matrices with different annealing temperatures.\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/893ca9d8c63cfda221f2d34a.png"},{"id":100363621,"identity":"1a330efd-682b-4e19-bcfa-9bf03fec4aea","added_by":"auto","created_at":"2026-01-16 07:50:46","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":56429,"visible":true,"origin":"","legend":"\u003cp\u003eIR spectroscopy for samples of materials of types I-IV with different annealing temperatures. A – Type I matrix, B – Type II matrix, C – Type III matrix, D – Type IV matrix\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/58a76a252b54021133bd6713.png"},{"id":100362122,"identity":"79ed6e19-fdad-41c3-b266-8e22c85f066f","added_by":"auto","created_at":"2026-01-16 07:46:12","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":199337,"visible":true,"origin":"","legend":"\u003cp\u003eDependence sorption of octenidine on time for I and II matrix types\u003c/p\u003e","description":"","filename":"6.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/8fb8a678302fb8b086b7ed01.png"},{"id":100041813,"identity":"1c9d15a8-7f2b-447c-90c5-b40f5d491f3d","added_by":"auto","created_at":"2026-01-12 11:21:20","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":299388,"visible":true,"origin":"","legend":"\u003cp\u003eDependence of amount of desorbed octenidine on material type and temperature.\u003c/p\u003e","description":"","filename":"7.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/2b19821f41d2839f3b09d603.png"},{"id":100381428,"identity":"db9edf51-0544-4fc3-bbd4-fb2c0a3e6631","added_by":"auto","created_at":"2026-01-16 10:38:52","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":3004455,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/8249cae0-422a-4094-8b55-2bcb9f0116aa.pdf"},{"id":100363269,"identity":"54124e4d-c6b9-4789-80ee-e789f83c92b3","added_by":"auto","created_at":"2026-01-16 07:49:15","extension":"png","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":309650,"visible":true,"origin":"","legend":"","description":"","filename":"GraphicalAbstract.png","url":"https://assets-eu.researchsquare.com/files/rs-8310871/v1/fcea8abaf201d24492b4e31f.png"}],"financialInterests":"No competing interests reported.","formattedTitle":"Microbial biotemplates for obtaining silica porous carrier for antibacterial substance","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003ePorous silicate materials represent a highly versatile class of inorganic matrices, which are distinguished by their ability to exhibit tunable texture, surface chemistry, and hierarchical porous structure [\u003cspan additionalcitationids=\"CR2\" citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]. At present, there is an increased demand for effective catalysts, adsorbents, membrane and filtration elements, sensors, drug delivery systems and materials for the energy sector, where controlled porosity and stability play a key role [\u003cspan additionalcitationids=\"CR5 CR6\" citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Sol-gel technology is a convenient method for synthesizing materials with customisable properties. It is characterized by its simplicity, environmentally friendly and rapid synthesis of non-toxic matrices, and the ability to obtain a variety of materials, such as thin films, membranes, powders, etc. [\u003cspan additionalcitationids=\"CR9\" citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. Sol-gel material has been found to exhibit a number of advantageous properties, which have resulted in its extensive utilization in a variety of fields. These include medicine, ecology, electrochemistry, and the formation of catalysts and biocatalysts [\u003cspan additionalcitationids=\"CR12 CR13 CR14 CR15 CR16\" citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. A notable area of interest lies in the utilization of sol-gel for the fabrication of sorbents [\u003cspan additionalcitationids=\"CR19 CR20\" citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e], carrier materials for antimicrobial substances [\u003cspan additionalcitationids=\"CR23\" citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e] and systems for delivering medicines.\u003c/p\u003e \u003cp\u003eIn order to obtain materials with controlled release of the substance, it is important to have a sufficiently large surface area and a developed pore system for diffusion of the active component. The template method, a widely utilized technique, facilitates the synthesis of micro- and mesoporous materials, characterized by an extensive surface area. However, the diffusion of the active substance may be limited by the presence of small-diameter pores, a phenomenon that is particularly pronounced when a sufficiently sterically loaded molecule is desorbed [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e]. Furthermore, cetylmethyltrimonium bromide (CTAB) is a frequently utilized template. This quaternary ammonium compound functions as a surfactant [\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e, \u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e]. However, CTAB is a toxic substance and may not be fully removed even by high-temperature treatment [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e]. Furthermore, during the synthesis of materials, there is a possibility of environmental accumulation, which would have a detrimental effect on the environment.\u003c/p\u003e \u003cp\u003eMicroorganism cells have been found to serve as an alternative to surfactant templates in the formation of porous materials. These cells are characterized by a wide variety of shapes, similar morphology among cells of the same strain, reproducibility of cell structure, environmental friendliness, and ease of biomass reproduction [\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e, \u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e]. In order to utilize microbial cells as templates, it is necessary to first encapsulate them completely in organosilicon material, and then to anneal them to form pores of a size corresponding to that of the microbial cells (typically 1\u0026ndash;3 micrometres). The sol-gel material formed around the microorganism cells has a developed porous structure, which has been previously studied in the process of forming a number of bioreceptor elements for the formation of biosensors [\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e, \u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e]. The development of a pore system enables the encapsulation of microorganisms within matrices, thereby preserving their catalytic activity and facilitating the acquisition of substrates through diffusion. This process also enables the elimination of waste products, thereby extending the operational lifespan of biosensors without the necessity of replacing the biomaterial. It has previously been established that each cell is immobilized individually, which is important for their use as templates [\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e, \u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eGram-positive and gram-negative bacteria of various shapes can be used as templates. \u003cem\u003eRhodococcus fascians\u003c/em\u003e (\u003cem\u003eR. fascians\u003c/em\u003e) are gram-positive, non-spore-forming bacteria measuring 1.0-2.5*0.5-1.0 \u0026micro;m, and capable of pleomorphism. In the exponential phase of active growth, the bacteria exhibit an elongated rod-like morphology. In the stationary phase, the bacteria acquire an oval shape, which is the predominant form for this particular type of microorganism. \u003cem\u003eR. fascians\u003c/em\u003e possesses a Gram-positive cell wall that exhibits characteristics consistent with those of mycobacteria, including the presence of mycolic acids. These acids have been demonstrated to facilitate the adsorption of precursor molecules and function as effective nucleation centres within the sol-gel process. Another template employed is \u003cem\u003ePseudomonas veronii\u003c/em\u003e, a Gram-negative rod-shaped non-spore-forming bacterium measuring 0.5-1.0 * 1.5-5.0 \u0026micro;m. The stable, rod-shaped form and small diameter of the fibres enable the creation of materials with a high specific surface area and an elongated, fibrous architecture. The outer membrane of \u003cem\u003eP. veronii\u003c/em\u003e, which is rich in lipopolysaccharides, provides a multitude of hydroxyl and carboxyl groups that act as centres for the chemical binding of organosilicon precursors. The utilization of Gram-positive and Gram-negative bacteria has been observed to result in the formation of matrices exhibiting diverse morphologies. This phenomenon has been demonstrated to impact the sorption and desorption properties of the matrices.\u003c/p\u003e \u003cp\u003eThe application of cells as biotemplates for the synthesis of porous carrier materials has the potential to enhance the sorption characteristics of matrices and mitigate diffusion-related challenges. This approach facilitates the regulated release of diverse active substances, including octenidine dihydrochloride, thereby achieving an antibacterial effect. Numerous studies utilize benzalkonium chloride [\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e, \u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e] and cetylpyridinium chloride as active agents [\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e, \u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e]. However, these compounds were initially described in the 1930s and are still widely employed today, despite their rapid loss of effectiveness due to the development of bacterial resistance [\u003cspan additionalcitationids=\"CR39\" citationid=\"CR38\" class=\"CitationRef\"\u003e38\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e]. The choice of octenidine as an antibacterial agent is predicated on several factors. Firstly, it is non-toxic, which makes it a suitable agent for use in humans. Secondly, it is capable of effectively combating both Gram-positive and Gram-negative microorganisms [\u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e, \u003cspan citationid=\"CR42\" class=\"CitationRef\"\u003e42\u003c/span\u003e]. The material will be more environmentally friendly, as the active component will be desorbed in small but effective concentrations. Furthermore, during the synthesis process, the toxic template (CTAB) will be replaced with biogenic templates based on bacterial cells.\u003c/p\u003e"},{"header":"2. Experimental part","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003e2.1 Reagents\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003eSilica sol\u0026thinsp;\u0026minus;\u0026thinsp;gel matrix based on methyltriethoxysilane (MTES) and tetraethoxysilane (TEOS) (Sigma\u0026thinsp;\u0026minus;\u0026thinsp;Aldrich, USA), and 5% aqueous PVA solution (FerakBerlin, Germany) was used as the basis for formation porous carrier with bacterial cells. Cetyltrimethylammonium bromide (CTAB) (Macklin, China) was used as a traditional surfactant template. Octenidine dihydrochloride (OCT) (Macklin, China) was used as the active antimicrobial agent.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003e2.2 Microorganism cultivation\u003c/h2\u003e \u003cp\u003eBacterial strains of the \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 and \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877 were obtained from the All-Russian collection of microorganisms (VKM) of the Institute of Biochemistry and Physiology of Microorganisms, Pushchino. Cultivation of the \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 was carried out on a Corynebacterium agar (Casein peptone 10.0 g; Yeast extract 5.0 g; Glucose 5.0 g; NaCl 5.0 g; Agar 15.0 g (Helicon, Russia)). Cultivation of the \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877 was carried out on a Peptone meat agar (Peptone 10.0 g; NaCl 5.0 g; Beef extract 3.0 g; Agar 20.0 g (Helicon, Russia)).\u003c/p\u003e \u003cp\u003eCultures of microorganisms were grown in flasks with a volume of 750 cm\u003csup\u003e3\u003c/sup\u003e (medium volume 200 cm\u003csup\u003e3\u003c/sup\u003e) at 28\u0026deg;C and aeration in a shaker at 180 rpm. The inoculum was introduced in an amount of 2% by volume of the medium to a final concentration of ~\u0026thinsp;10\u003csup\u003e8\u003c/sup\u003e CFU/cm\u003csup\u003e3\u003c/sup\u003e. The grown biomass was centrifuged at 10000 rpm for 10 min, and the precipitate was washed with phosphate buffer solution (20 mmol/dm\u003csup\u003e3\u003c/sup\u003e, pH 6.8). Cell biomass was stored in microtubes at \u0026minus;\u0026thinsp;15\u0026deg;C.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003e2.3 Formation of organosilicon biohybrid material\u003c/h2\u003e \u003cp\u003e \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 and \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877 bacteria strains were used as biotemplates to obtain materials of types I and II, respectively. To synthesize the silicon material, 172 \u0026micro;l of a 5% polyvinyl alcohol solution was added to a 430 \u0026micro;l cell suspension (1.0\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1 \u0026times; 10⁹ CFU/ml) in a phosphate buffer solution (pH 6.8), which was then mixed for five minutes using an Elmi CM-70M07 mixer (Riga, Latvia). Then, add a mixture of methyltriethoxysilane and tetraethoxysilane in volume ratios of 50/50 to the mixture. Stir the mixture at 100 rpm for 5 minutes. Then added 43 \u0026micro;l basic catalysts 0.2 M NaF and stir at 100 rpm for 15 minutes. Leave to dry in air at room temperature for 24 hours.\u003c/p\u003e \u003cp\u003eThe synthesis of materials of types III and IV was achieved by the addition of 170 mg of cetyltrimethylammonium bromide (CTAB) and 50 mg of NaOH (Helicon, Russia) to 80 ml of distilled water at 80\u0026deg;C. The mixture was stirred until complete dissolution occurred. Subsequently, 417 \u0026micro;l of methyltriethoxysilane and 417 \u0026micro;l of tetraethoxysilane (for type III) or 834 \u0026micro;l of tetraethoxysilane (for type IV) were added dropwise and stirred for 2 hours. Thereafter, the mixture was precipitated in a centrifuge at a speed of 10,000 rpm for 5 minutes. The specimens were then subjected to a thorough cleansing process involving distilled water, followed by drying in an oven at a precise temperature of 90\u0026deg;C.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec6\" class=\"Section2\"\u003e \u003ch2\u003e2.4 Removal of templates from the formed material\u003c/h2\u003e \u003cp\u003eMaterials of types I\u0026ndash;IV, formed using templates, were then annealed in an oxygen atmosphere at a heating rate of 10\u0026deg;C per minute at temperatures of 200, 400, 600, 800, 1000 and 1200\u0026deg;C, with a 1-hour hold at the set temperature.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003e2.5 Scanning electron microscopy (SEM)\u003c/h2\u003e \u003cp\u003ePrior to measurements, samples encapsulated in a silica sol-gel matrix of \u003cem\u003eRhodococcus fascians and Pseudomonas veronii\u003c/em\u003e cells were mounted on a 25 mm diameter aluminum pin, fixed with conductive carbon adhesive tape and covered with a thin film (15 nm) of carbon. Observations were conducted using a Hitachi TM4000Plus scanning electron microscope. Images were acquired in secondary electron mode at an accelerating voltage of 10 kV. EDX-SEM studies were performed using an Oxford Instruments X-max 80 EDX system at an accelerating voltage of 10 kV.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003e2.6 IR spectroscopy\u003c/h2\u003e \u003cp\u003eIR spectra of materials were recorded on a Fourier infrared spectrometer FMS 1201 (Monitoring, St. Petersburg, Russia). The samples and pure potassium bromide were dried for 2 hours in a desiccator at 90 degrees Celsius. The spectra of the solids were measured using KBr discs. A solid sample (3 mg) was thoroughly mixed with potassium bromide (150\u0026ndash;200 mg) in a mortar and the mixture was pressed at a pressure of 10 t/cm\u0026sup2; for 3 min. The resulting sample spectrum was recorded relative to the background air spectrum.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003e2.7 Sorption of octenidine dihydrochloride\u003c/h2\u003e \u003cp\u003eIn order to study the desorption properties of the formed materials, 20 milligrams of the sample was placed in 4 milliliters of octenidine dihydrochloride solution (titer \u0026minus;\u0026thinsp;5 mg/ml) for a different period of time at 180 rpm. Subsequently, the materials were separated by centrifugation (10,000 rpm, 5 min). The upper layer was then decanted and analyzed, after which the material was dried at 85\u0026deg;C for a period of two hours.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec10\" class=\"Section2\"\u003e \u003ch2\u003e2.8 Study of desorption capacity\u003c/h2\u003e \u003cp\u003eAt 30-minute intervals, 5 mg of the substance was deposited into a test tube, followed by the addition of 30 ml of distilled water. The mixture was then subjected to a centrifugal process at 10,000 rpm for a duration of 10 minutes. Two milliliters of the solution were placed into a quartz cuvette, after which the optical density was measured at a wavelength of 280 nm.\u003c/p\u003e \u003c/div\u003e"},{"header":"3. Results and Discussions","content":"\u003cp\u003eThe following four categories of material were formed. The formation of materials of types I and II involved the use of bacterial cells, specifically \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 and \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877, as biotemplates (Fig. \u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e). These two strains of microorganisms display significant disparities in morphology, a factor that has the potential to influence the materials obtained and their sorption capacity.\u003c/p\u003e\n\u003cp\u003eThe material employed for the formation of the silica shells was composed of methyltriethoxysilane and tetraethoxysilane in a volume ratio of 50/50 vol%, a proportion that had been determined to be more effective for complete encapsulation of bacteria [\u003cspan class=\"CitationRef\"\u003e31\u003c/span\u003e]. As comparison samples, type III and IV materials were synthesized using cetyltrimethylammonium bromide micelles as templates, using methyltriethoxysilane and tetraethoxysilane 50/50 vol.%, as well as 100 vol.% TEOS. The compositions under scrutiny were selected to elucidate the discrepancy between the utilization of biotemplates and CTAB, as well as a conventional material based on tetraethoxysilane in combination with CTAB.\u003c/p\u003e\n\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\n \u003ch2\u003e3.1 Determination of the morphology of silicon-containing materials obtained using various templates\u003c/h2\u003e\n \u003cp\u003eIn the initial phase of the research, a comprehensive array of materials was subjected to a thermal treatment within the temperature range of 200\u0026ndash;1200\u0026deg;C. It is evident that all samples under consideration are fine-grained materials. The matrices that were not subjected to heat treatment are white in color. It has been established that when the materials are subjected to annealing at temperatures of 200 and 400\u0026deg;C, they exhibit a brown coloration. However, at a temperature of 600\u0026deg;C, a transformation to a light grey hue is observed. Samples of types I and II exhibit white coloration at temperatures in excess of 800\u0026deg;C. Materials of types III and IV, when subjected to temperatures ranging from 800 to 1200\u0026deg;C, exhibit a light grey hue accompanied by dark grey spots. This phenomenon may be attributed to the incomplete annealing of cetyltrimethyl ammonium bromide (CTAB) micelles. This suggests the presence of residual carbon within the materials, as illustrated in Fig. \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e.\u003c/p\u003e\n \u003cp\u003eThe morphology of the materials is influenced by the type of template used. Type I samples that have not undergone annealing typically exhibit a plate-like appearance; however, upon reaching 800\u0026deg;C, they typically acquire a powdery appearance. Type II materials consist of thicker (up to 2 mm) platelets that retain their structure even after annealing at 1200\u0026deg;C. This phenomenon may be attributed to variations in the composition of the cell wall of gram-positive and gram-negative bacteria, including the number of hydroxyl groups present. Materials obtained using CTAB micelles exhibit no change in appearance, forming fine powders.\u003c/p\u003e\n \u003cp\u003eIn the subsequent stage of the research, the morphology of the obtained materials was studied using scanning electron microscopy in order to investigate the influence of templates and annealing temperature (Fig. \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e).\u003c/p\u003e\n \u003cp\u003eMaterials formed using microorganism cells consist of particles remaining after encapsulation of bacteria. \u003cem\u003eRhodococcus fascians\u003c/em\u003e cells were used for sample type I, and when immobilized, silicon-containing shells with average dimensions of 1\u0026times;1.6 \u0026micro;m are formed. In the process of forming type II material using \u003cem\u003ePseudomonas veronii\u003c/em\u003e cells, the formation of more elongated cylindrical particles with dimensions of 1\u0026times;3.5 \u0026micro;m is observed, which corresponds to the description of their morphology. It has been established that at annealing temperatures of 200 and 400\u0026deg;C, rough materials exhibit uneven areas of irregular shape. At a temperature of 600\u0026deg;C, materials of types I and II are characterized by a smoother folded layer with cylindrical particles. Prior to the initiation of the melting process, the dimensions of the cylindrical particles remain constant. Type III material is formed from small spherical particles that are joined together, and this morphology is observed at annealing temperatures ranging from 200 to 600\u0026deg;C. Type IV material is more uniform than type III material. This phenomenon may be attributed to the use of a single silane precursor, tetraethoxysilane, which facilitates both hydrolysis and polycondensation reactions across all four Si-O bonds. At the same time, the introduction of a second silane precursor, methyltriethoxysilane, into the type III material allows for a more heterogeneous surface to be obtained, which may have a more developed surface area, and consequently affect the sorption properties.\u003c/p\u003e\n \u003cp\u003eAll materials are characterized by matrix fusion at annealing temperatures ranging from 800 to 1200\u0026deg;C. It has been demonstrated that at 800\u0026deg;C, materials formed using bacterial cells become more porous, and cylindrical particles on the surface disappear. It is evident that an increase in temperature to 1200\u0026deg;C results in a further enhancement of the samples\u0026apos; smoothness. The type I matrix sample is a smooth material with round visible cavities. Materials of types II and III are characterized by their heterogeneity, with pores of varying sizes. At an elevated temperature of 1200\u0026deg;C, the type IV material exhibits a monolithic structure, devoid of any discernible pores on the surface. The morphology of materials obtained by employing different templates can exhibit notable variation, which can subsequently result in divergent sorption and desorption properties when utilized as loading platforms.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\n \u003ch2\u003e3.2 Confirmation of silicon carrier formation using IR spectroscopy\u003c/h2\u003e\n \u003cp\u003eIn order to confirm the formation of polymeric silicon-containing materials, their samples were studied using IR spectroscopy (Fig. \u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003e). The materials are characterized by peaks that are indicative of the formation of Si-O-Si bonds. The 800 cm\u003csup\u003e-1\u003c/sup\u003e spectrum is indicative of inter-tetrahedral vibrations, while the 1075 and 1100 cm\u003csup\u003e-1\u003c/sup\u003e peaks correspond to stretching vibrations.\u003c/p\u003e\n \u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n \u003cp\u003eDespite annealing, all materials demonstrate an retention of CH\u003csub\u003e3\u003c/sub\u003e groups, being part of methyltriethoxysilane, which exhibit stretching vibrations at 1265 (1285) cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e and bending vibrations at 1400 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e. However, it is observed that the intensity of these vibrations decreases with increasing temperature. The presence of bonds at 1650 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e is indicative of the occurrence of bending vibrations of OH groups. Furthermore, for materials of types III and IV, at processing temperatures up to 600\u0026deg;C, peaks with high intensity at 2850 and 2965 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e are evident. These vibrations may be indicative of asymmetric and symmetric stretching vibrations of CTAB. [\u003cspan class=\"CitationRef\"\u003e43\u003c/span\u003e]. Furthermore, at temperatures up to 400\u0026deg;C, asymmetric stretching vibrations of N\u003csup\u003e+\u003c/sup\u003e(CH\u003csub\u003e3\u003c/sub\u003e)\u003csub\u003e3\u003c/sub\u003e groups and insignificant vibrations of bromide anions at 720 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e are observed, which suggests that up to 600\u0026deg;C, cetyltrimethylammonium bromide remains in the material, as described in the literature, which may have a negative effect on the loading capacity of the matrices [\u003cspan class=\"CitationRef\"\u003e28\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e44\u003c/span\u003e].\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec14\" class=\"Section2\"\u003e\n \u003ch2\u003e3.3 Study of the sorption and desorption properties of materials\u003c/h2\u003e\n \u003cp\u003eOctenidine dihydrochloride, a quaternary ammonium compound with proven antiseptic efficacy, was applied to the obtained material samples [\u003cspan class=\"CitationRef\"\u003e42\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e45\u003c/span\u003e]. To determine the sorption time, materials of types I and II were used, which showed the best loading capacity in preliminary studies (Fig. \u003cspan class=\"InternalRef\"\u003e5\u003c/span\u003e). The dependence of octenidine sorption in materials on the exposure time of samples in a concentrated solution was determined using UV spectroscopy.\u003c/p\u003e\n \u003cp\u003eIt has been determined that the optimal time for sorption of these materials is 1 hour, after which octenidine desorbs from the surface of the samples into the solution. Subsequent studies were conducted with sorption for a duration of one hour. Furthermore, the desorption properties of the materials were the subject of study. The results of the study are presented in Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e\n \u003cdiv class=\"gridtable\"\u003e\u0026nbsp;\u003ctable id=\"Tab1\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eCharacteristics of the formed materials\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003ccolgroup cols=\"4\"\u003e\u003c/colgroup\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003eMatrix\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eOСT loading, \u0026micro;g/1 mg of carrier\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eCumulative release of OCT from 1 mg of carrier, \u0026micro;g\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"4\"\u003e\n \u003cp\u003e200 \u0026deg;С\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eI type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e183\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e146\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e199\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e27\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIV type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e213\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e27\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"4\"\u003e\n \u003cp\u003e400 \u0026deg;С\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eI type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e217\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e29\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e229\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e32\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e269\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e33\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIV type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e253\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e31\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"4\"\u003e\n \u003cp\u003e600 \u0026deg;С\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eI type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e303\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e87\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e268\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e53\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e299\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e63\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIV type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e311\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e67\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"4\"\u003e\n \u003cp\u003e800 \u0026deg;С\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eI type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e\u003cstrong\u003e376\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e\u003cstrong\u003e130\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e\u003cstrong\u003e340\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e\u003cstrong\u003e94\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e318\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e73\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIV type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e327\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e69\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"4\"\u003e\n \u003cp\u003e1000 \u0026deg;С\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eI type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e271\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e56\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e253\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e46\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e176\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e28\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIV type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e183\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e30\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"4\"\u003e\n \u003cp\u003e1200 \u0026deg;С\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eI type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e277\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e321\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e23\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIII type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e294\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e19\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eIV type\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e302\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n \u003cp\u003eFor silicon-containing matrices of all types, an increase in sorption and desorption characteristics is observed with rising temperature. The highest values for octenidine loading and release are achieved for materials with an annealing temperature of 800\u0026deg;C. This can be attributed to changes in the morphology of the samples upon reaching the Tammann temperature for silicates (759\u0026deg;C). An further increase in temperature has been shown to result in the fusion of the matrices, which has a negative effect on their characteristics. Materials obtained using biotemplates have been shown to exhibit high values of adsorbed and desorbed octenidine.\u003c/p\u003e\n \u003cp\u003eThe highest values of octenidine sorption and desorption are observed in type I matrix formed using \u003cem\u003eRh. fascians\u003c/em\u003e cells (Fig. \u003cspan class=\"InternalRef\"\u003e6\u003c/span\u003e) and annealing temperature of 800\u0026deg;C. This phenomenon may be attributed to the formation of material with sufficiently large transport pores, thereby enabling octenidine to desorb without diffusion difficulties. The kinetic curves for octenidine release were plotted for materials of all types with different annealing temperatures (Fig. \u003cspan class=\"InternalRef\"\u003e7\u003c/span\u003e). The release profiles for materials that have undergone annealing at 800\u0026deg;C are distinguished by the presence of two distinct stages. The initial stage is characterized by a short-term release effect, while the subsequent stage is marked by a more prolonged release, exhibiting an approximate linear dependence. For types I, III, IV samples at 600\u0026deg;C, a similar release pattern is observed, but with lower cumulative release values.\u003c/p\u003e\n \u003cp\u003eMaterials formed using CTAB are characterised by the release of smaller amounts of octenidine, which may be due to the morphology and preservation of larger amounts of carbon after annealing. The size of CTAB micelles is 2\u0026ndash;3 nm, which is comparable to the size of the octenidine molecule (0.9 nm). This has the effect of causing diffusion difficulties during the desorption of the active substance. Kinetic models (zero order, first order, Korsmeyer-Peppas) were utilised to characterise the release of octenidine from all materials at an annealing temperature of 800\u0026deg;C (Table \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e\n \u003cp\u003e\u003cimg src=\"data:image/png;base64,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\" width=\"622\" height=\"601\"\u003e\u003c/p\u003e\n \u003cp\u003eThe data processing using a modified Korsmeyer-Peppas model that takes into account the burst effect is characterised by high correlation coefficients. For all materials in the first stage with a burst effect, the diffusion exponents \u003cem\u003en\u003c/em\u003e\u003csub\u003e\u003cem\u003eI\u003c/em\u003e\u003c/sub\u003e of the Korsmeyer-Peppas model range from 0.37 to 0.57, which is characteristic of Fickian diffusion-controlled release. The release constants \u003cem\u003ek\u003c/em\u003e\u003csub\u003e\u003cem\u003eI\u003c/em\u003e\u003c/sub\u003e in the first stage exceed the release constants in the second stage to a significant degree, thus confirming two-stage desorption. In stage I, more than 20% of octenidine is rapidly released from the surface of materials or from large transport pores. The diffusion flow is directed towards the concentration gradient, and diffusion difficulties are minimal. Type I material is characterised by the highest constant.\u003c/p\u003e\n \u003cp\u003eIn the second stage, the diffusion exponent values exceed 0.43 for all materials, indicating abnormal transport release occurring according to non-Fickian diffusion. Various diffusion difficulties arise due to the release of octenidine from the thickness of the materials, the desorption rate decreases, and re-adsorption of octenidine on the surface is possible due to the presence of OH groups in the matrices, as well as the occurrence of steric difficulties due to the peculiarities of the morphology of the materials. The values of the constants are significantly lower than in stage I of release.\u003c/p\u003e\n \u003cp\u003eDespite the use of different ratios of silane precursors, there is a insignificant difference in the sorption and desorption properties of materials of types III and IV. This may indicate that the nature of the template has a great influence. The utilisation of \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 and \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877 bacterial cells as biotemplates resulted in a 1.3\u0026ndash;1.7 fold increase in the desorption of octenidine compared to materials formed using cetyl trimethyl ammonium bromide (130 and 94 \u0026micro;g of octenidine/mg of carrier for bacterial templates, 73 and 69 \u0026micro;g of octenidine/mg of carrier for surfactants).Consequently, bacterial cells can be utilized as a viable alternative to conventional templates. The employment of these biotemplates has the potential to facilitate the formation of porous materials for sorption, drug delivery systems, catalyst carriers, and antibacterial materials\u003c/p\u003e\n\u003c/div\u003e"},{"header":"4. Conclusion","content":"\u003cp\u003eThe application of microbial cells as biological templates for the synthesis of silica has been shown to be an effective method for the controlled release of octenidine. The optimal heat treatment was determined to be annealing at 800\u0026deg;C, a process which ensured the removal of the biological template without sintering the silicate matrix. At lower temperatures, templates persist within the samples, thereby impacting the loading of octenidine. It is evident that an increase in temperature results in material fusion. This phenomenon has a subsequent impact on the pore volume, which is reduced, and the sorption capacity of the matrices, which is negatively affected. The optimal time for maximum adsorption of octenidine (18%) was determined to be 1 hour, after which the substance desorbs into the solution. An increase in adsorbed octenidine is observed when the annealing temperature of the materials is raised to 800\u0026deg;C, after which there is a decrease in loading capacity, a characteristic of all samples.\u003c/p\u003e \u003cp\u003eThe highest adsorption was observed for the matrix obtained using Gram-positive bacteria \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM Ac-1462. A comparative analysis demonstrated the advantage of the Gram-positive bacterium \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 over the Gram-negative \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877 when used as biotemplates. The rigid cell wall of \u003cem\u003eR. fascians\u003c/em\u003e, stabilized by mycolic acids, retained its structural integrity during the ageing and drying of the sol-gel material. This ensured the formation of a porous surface with higher sorption characteristics. In contrast, the thin cell wall of \u003cem\u003eP. veronii\u003c/em\u003e was likely to undergo rapid deformation during xerogel formation. The elongated shape of \u003cem\u003eP. veronii\u003c/em\u003e also contributes to the sorption properties of the material, leading to the formation of an irregular pore structure with defects.\u003c/p\u003e \u003cp\u003eIn the process of annealing materials formed using CTAB, the quaternary ammonium compound is known to remain in the matrices. This phenomenon has been confirmed through the analysis of infrared spectroscopy data. The presence of CTAB in samples III and IV has been shown to affect the sorption and desorption of octenidine. This, in turn, requires a change in the procedure for removing surfactants from silicate material in order to improve the properties of the materials.\u003c/p\u003e \u003cp\u003eThe kinetic curves obtained demonstrate that matrices with an annealing temperature of 800\u0026deg;C are characterised by two-phase desorption, in which the initial stage involves the explosive release of the active substance from the surface of the samples, followed by a phase of prolonged release from the depths of the material. It was determined that the desorption of octenidine in the initial phase is in accordance with Fick's diffusion theory. The mechanism of desorption in the second stage is non-Fickian diffusion; the release of octenidine is slowed down due to diffusion difficulties caused by the possible re-adsorption of octenidine in the narrow pores of the matrices.\u003c/p\u003e \u003cp\u003eIn comparison with the CTAB template, the employment of microbial cells has the potential to offer a number of applications due to cost-effectiveness, environmental safety, and ease of removal of the pore-forming agent. The results confirm the hypothesis that biological templates can be utilized to create functional porous materials for the controlled delivery of antiseptic agents and drugs. Subsequent research will concentrate on the study of the antimicrobial activity of the obtained materials, the testing of biocompatibility, and the investigation of the possibility of adding alternative antiseptics and medicinal products.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e \u003ch2\u003eConflict of Interest\u003c/h2\u003e \u003cp\u003eThe authors declare no competing financial interest.\u003c/p\u003e \u003c/p\u003e\u003ch2\u003eFunding\u003c/h2\u003e \u003cp\u003eThis research was funded by grants from the Russian Science Foundation, RSF № 25-23-00410, \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://rscf.ru/en/project/25-23-00410/\u003c/span\u003e\u003cspan address=\"https://rscf.ru/en/project/25-23-00410/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e.\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eAll authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by EAL, PVA and KDA. The first draft of the manuscript was written by EAL and OAK, all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eRashid AB, Shishir SI, Mahfuz MA, Hossain MT, Hoque ME (2023) Silica aerogel: synthesis, characterization, applications, and recent advancement. Part Part Syst Charact. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1002/ppsc.202200186\u003c/span\u003e\u003cspan address=\"10.1002/ppsc.202200186\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNayl AA, Abd-Elhamid AI, Aly AA, Br\u0026auml;se S (2022) Recent progress in the applications of silica-based nanoparticles. RSC Adv. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1039/D2RA01587K\u003c/span\u003e\u003cspan address=\"10.1039/D2RA01587K\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCiriminna R, Pagliaro M (2022) Open challenges in sol\u0026ndash;gel science and technology. J Sol-Gel Sci Technol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1007/s10971-021-05535-9\u003c/span\u003e\u003cspan address=\"10.1007/s10971-021-05535-9\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYu X, Williams CT (2022) Recent advances in the applications of mesoporous silica in heterogeneous catalysis. Catal Sci Technol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1039/D2CY00001F\u003c/span\u003e\u003cspan address=\"10.1039/D2CY00001F\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eShinde PS, Suryawanshi PS, Patil KK, Belekar VM, Sankpal SA, Delekar SD, Jadhav SA (2021) A brief overview of recent progress in porous silica as catalyst supports. J Compos Sci. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.3390/jcs5030075\u003c/span\u003e\u003cspan address=\"10.3390/jcs5030075\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWang J, Liu Y, Cai Q, Dong A, Yang D, Zhao D (2022) Hierarchically porous silica membrane as separator for high-performance lithium‐ion batteries. Adv Mater. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1002/adma.202107957\u003c/span\u003e\u003cspan address=\"10.1002/adma.202107957\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOh JY, Yang G, Choi E, Ryu JH (2022) Mesoporous silica nanoparticle-supported nanocarriers with enhanced drug loading, encapsulation stability, and targeting efficiency. Biomater Sci. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1039/D2BM00010E\u003c/span\u003e\u003cspan address=\"10.1039/D2BM00010E\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eEmrie DB (2024) Sol\u0026ndash;gel synthesis of nanostructured mesoporous silica powder and thin films. J Nanomater. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1155/2024/6109770\u003c/span\u003e\u003cspan address=\"10.1155/2024/6109770\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLionello DF, Ramallo JI, Soler-Illia GJ, Fuertes MC (2022) Mechanical properties of ordered mesoporous oxides thin films. J Sol-Gel Sci Technol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1007/s10971-021-05626-7\u003c/span\u003e\u003cspan address=\"10.1007/s10971-021-05626-7\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKurt T, Topuz B (2021) Sol-gel control on mixed network silica membranes for gas separation. Sep Purif Technol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.seppur.2020.117654\u003c/span\u003e\u003cspan address=\"10.1016/j.seppur.2020.117654\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKamanina OA, Lantsova EA, Rybochkin PV, Arlyapov VA, Plekhanova YV, Reshetilov AN (2022) The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices. Membranes. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.3390/membranes12100983\u003c/span\u003e\u003cspan address=\"10.3390/membranes12100983\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eFotie J (2025) Applications of tailored mesoporous silicate nanomaterials in regenerative medicine and theranostics /. Int J Mol Sci. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.3390/ijms26167918\u003c/span\u003e\u003cspan address=\"10.3390/ijms26167918\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWang G, Xu X, Cheng Q, Hu J, Xu X, Zhang Y et al (2023) Preparation of sustainable release mesoporous silica nano-pesticide for control of Monochamus alternatus. Sustain Mater Technol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.susmat.2022.e00538\u003c/span\u003e\u003cspan address=\"10.1016/j.susmat.2022.e00538\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eFan X, Wu J, Zhang T, Liu J (2024) Electrochemical/electrochemiluminescence sensors based on vertically-ordered mesoporous silica films for biomedical analytical applications. ChemBioChem. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1002/cbic.202400320\u003c/span\u003e\u003cspan address=\"10.1002/cbic.202400320\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKamanina OA, Lantsova EA, Rybochkin PV (2025) Efficient Approach to the Immobilization of Glucose Oxidase in Sol-Gel Polymeric Matrices Using Silane Precursors with One and Two Non-Hydrolyzable Bonds J. Anal Chem. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1134/S1061934825700583\u003c/span\u003e\u003cspan address=\"10.1134/S1061934825700583\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eEkeoma BC, Yusuf M, Johari K, Abdullah B (2022) Mesoporous silica supported Ni-based catalysts for methane dry reforming: A review of recent studies. Int J Hydrogen Energy. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.ijhydene.2022.05.297\u003c/span\u003e\u003cspan address=\"10.1016/j.ijhydene.2022.05.297\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDong W, Yan J, Yang Y, Wu Q, Hu X (2024) Immobilization of laccase on magnetic mesoporous silica as a recoverable biocatalyst for the efficient degradation of benzo [a] pyrene // Chemosphere. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.chemosphere.2023.140642\u003c/span\u003e\u003cspan address=\"10.1016/j.chemosphere.2023.140642\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDruzian DM, Da Silva MDC, Da Silva WL, Santos JHZD (2025) Sol-Gel Technologies for Drug Delivery in Potential Cancer Treatments. Novel Approaches in Cancer Treatment: Tumor Targeted Therapy. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1007/978-3-032-00771-1_397\u003c/span\u003e\u003cspan address=\"10.1007/978-3-032-00771-1_397\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhu H, Zheng K, Boccaccini AR (2021) Multi-functional silica-based mesoporous materials for simultaneous delivery of biologically active ions and therapeutic biomolecules. Acta Biomater. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.actbio.2021.05.007\u003c/span\u003e\u003cspan address=\"10.1016/j.actbio.2021.05.007\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePellicer-Castell E, Belenguer-Sapi\u0026ntilde;a C, Amoros P, El Haskouri J, Herrero-Mart\u0026iacute;nez JM, Mauri-Aucejo AR (2022) Mesoporous silica sorbent with gold nanoparticles for solid-phase extraction of organochlorine pesticides in water samples. J Chromatogr A. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.chroma.2021.462729\u003c/span\u003e\u003cspan address=\"10.1016/j.chroma.2021.462729\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRipon RI, Begum ZA, Miyazawa R, Leonard K, Ogata M, Ahmmad B, Rahman IM (2024) Development and application of a new macrocyclic ligand-functionalized mesoporous silica sorbent for selective separation of radiocesium from environmental wastewater. Microchem J. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.microc.2024.111649\u003c/span\u003e\u003cspan address=\"10.1016/j.microc.2024.111649\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNamdar N, Nayeri Fasaei B, Shariati P, Joghataei SM, Arpanaei A (2024) Mesoporous silica nanoparticles co-loaded with lysozyme and vancomycin for synergistic antimicrobial action. Sci Rep Nat. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1038/s41598-024-78922-1\u003c/span\u003e\u003cspan address=\"10.1038/s41598-024-78922-1\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAlharthi S, Ziora ZM, Janjua T, Popat A, Moyle PM (2022) Formulation and Biological Evaluation of Mesoporous Silica Nanoparticles Loaded with Combinations of Sortase A Inhibitors and Antimicrobial Peptides. Pharmaceutics. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.3390/pharmaceutics14050986\u003c/span\u003e\u003cspan address=\"10.3390/pharmaceutics14050986\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLantsova EA, Pertseva VA, Soromotin VN, Saverina EA, Kamanina OA (2025) Functionalized Organosilicon Material as a Potential Support for the Loading of Active Compounds. Silicon. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1007/s12633-025-03293-9\u003c/span\u003e\u003cspan address=\"10.1007/s12633-025-03293-9\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eBarman S, Fager C, R\u0026ouml;ding M et al (2021) New Characterization Measures of Pore Shape and Connectivity Applied to Coatings used for Controlled Drug Release. J Pharm Sci. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.xphs.2021.02.0242021\u003c/span\u003e\u003cspan address=\"10.1016/j.xphs.2021.02.0242021\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eBeltr\u0026aacute;n-Aburto R, Borjas-Garc\u0026iacute;a SE, Mart\u0026iacute;nez-Torres P, Villegas J, Medina Flores A (2025) Synthesis of Mesoporous Samarium Oxide by Using CTAB. Microsc Microanal. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1093/mam/ozaf048.1054\u003c/span\u003e\u003cspan address=\"10.1093/mam/ozaf048.1054\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDe Araujo BRS, Onrubia-Calvo JA, Stambouli I et al (2022) Towards the development of advanced hierarchical chabazite materials: Novel micro-mesoporous silicoaluminophosphate SAPO-34 zeolites. Mater Today Commun. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.mtcomm.2022.103580\u003c/span\u003e\u003cspan address=\"10.1016/j.mtcomm.2022.103580\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCarvalho GC, Marena GD, Karnopp JCF et al (2022) Cetyltrimethylammonium bromide in the synthesis of mesoporous silica nanoparticles: General aspects and in vitro toxicity. Adv Colloid Interface Sci. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.cis.2022.102746\u003c/span\u003e\u003cspan address=\"10.1016/j.cis.2022.102746\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSrujana TL, Rao KJ, Korumilli T (2025) Natural Biogenic Templates for Nanomaterial Synthesis: Advances, Applications, and Environmental Perspectives. ACS Biomater Sci Eng. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1021/acsbiomaterials.4c02075\u003c/span\u003e\u003cspan address=\"10.1021/acsbiomaterials.4c02075\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOh D, Ozgit-Akgun C, Akca E et al (2017) Biotemplating pores with size and shape diversity for Li-oxygen Battery Cathodes. Sci Rep. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1038/srep45919\u003c/span\u003e\u003cspan address=\"10.1038/srep45919\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRybochkin PV, Kamanina OA, Lantsova EA, Arlyapov VA, Saverina EA (2023) Characterization of the catalytic ability and surface properties of a heterogeneous biocatalyst obtained by the sol-gel method. J Sol-Gel Sci Technol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1007/s10971-022-05915-9\u003c/span\u003e\u003cspan address=\"10.1007/s10971-022-05915-9\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKamanina OA, Lantsova EA, Rybochkin PV et al (2023) 3-in-1 Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol\u0026ndash;Gel Matrix for Determining Sewage Pollution. ACS Appl Mater Interfaces. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1021/acsami.3c09897\u003c/span\u003e\u003cspan address=\"10.1021/acsami.3c09897\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLantsova EA, Kamanina OA, Rybochkin PV, Arlyapov VA, Saverina EA, Vereshchagin AN (2024) Immobilization of microbial cells in a sol-gel matrix using a precursor with two non-hydrolyzed bonds. J Sol-Gel Sci Technol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1007/s10971-023-06275-8\u003c/span\u003e\u003cspan address=\"10.1007/s10971-023-06275-8\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhang X, Li S, Deng Y, Zuo Z, Sun Z, Li C, Zheng S (2023) Benzalkonium chloride modified kaolinite with rapid bactericidal activity for the development of antibacterial films. Mater Today Commun. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.mtcomm.2023.106152\u003c/span\u003e\u003cspan address=\"10.1016/j.mtcomm.2023.106152\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKhelissa S, Gharsallaoui A, Fadel A et al (2021) Microencapsulation of benzalkonium chloride enhanced its antibacterial and antibiofilm activities against Listeria monocytogenes and Escherichia coli. J Appl Microbiol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1111/jam.15010\u003c/span\u003e\u003cspan address=\"10.1111/jam.15010\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKhan S, Amin F, Amin R, Kumar N (2024) Exploring the Effect of Cetylpyridinium Chloride Addition on the Antibacterial Activity and Surface Hardness of Resin-Based Dental Composites. Polymers. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.3390/polym16050588\u003c/span\u003e\u003cspan address=\"10.3390/polym16050588\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCabral-Romero C, Hern\u0026aacute;ndez-Delgadillo R, Nakagoshi-Cepeda SE et al (2024) Antimicrobial and antitumor activities of an alginate-based membrane loaded with bismuth nanoparticles and cetylpyridinium chloride. J Appl Biomater Funct Mater. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1177/22808000241236590\u003c/span\u003e\u003cspan address=\"10.1177/22808000241236590\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKim M, Weigand MR, Oh S et al (2018) Widely Used Benzalkonium Chloride Disinfectants Can Promote Antibiotic Resistance. Appl Environ Microbiol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1128/AEM.01201-18\u003c/span\u003e\u003cspan address=\"10.1128/AEM.01201-18\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMao X, Auer DL, Buchalla W et al (2020) Cetylpyridinium Chloride: Mechanism of Action, Antimicrobial Efficacy in Biofilms, and Potential Risks of Resistance. Antimicrob Agents Chemother. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1128/aac.00576-20\u003c/span\u003e\u003cspan address=\"10.1128/aac.00576-20\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKampf G (2024) Benzalkonium Chloride BT - Antiseptic Stewardship: Biocide Resistance and Clinical Implications / ed. Kampf G. Springer International Publishing, Cham\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKoeck R, Denkel L, Fessler AT et al (2023) Clinical Evidence for the Use of Octenidine Dihydrochloride to Prevent Healthcare-Associated Infections and Decrease Staphylococcus aureus Carriage or Transmission\u0026mdash;A. Rev Pathogens. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.3390/pathogens12040612\u003c/span\u003e\u003cspan address=\"10.3390/pathogens12040612\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRzycki M, Drabik D, Szostak-Paluch K et al (2021) Unraveling the mechanism of octenidine and chlorhexidine on membranes: Does electrostatics matter? Biophys. J. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.bpj.2021.06.027\u003c/span\u003e\u003cspan address=\"10.1016/j.bpj.2021.06.027\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAi F, Zhao G, Lv W, Lin J (2020) Facile synthesis of cetyltrimethylammonium bromide-loaded mesoporous silica nanoparticles for efficient inhibition of hepatocellular carcinoma cell proliferation. Mater Res Express. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1088/2053-1591/abad15\u003c/span\u003e\u003cspan address=\"10.1088/2053-1591/abad15\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHan HW, Joe A, Jang ES (2021) Reduced cytotoxicity of CTAB-templated silica layer on gold nanorod using fluorescence dyes and its application in cancer theranostics. J Ind Eng Chem. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1016/j.jiec.2021.01.020\u003c/span\u003e\u003cspan address=\"10.1016/j.jiec.2021.01.020\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMalanovic N, Buttress JA, Vejzovic D et al (2022) Disruption of the Cytoplasmic Membrane Structure and Barrier Function Underlies the Potent Antiseptic Activity of Octenidine in Gram-Positive Bacteria. Appl Environ Microbiol. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://doi.org/10.1128/aem.00180-22\u003c/span\u003e\u003cspan address=\"10.1128/aem.00180-22\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"silicon","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scon","sideBox":"Learn more about [Silicon](https://www.springer.com/journal/12633)","snPcode":"12633","submissionUrl":"https://submission.nature.com/new-submission/12633/3","title":"Silicon","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"sol-gel, silicon сarrier, biotemplate, octenidine dehydrochloride, antibacterial, porous material","lastPublishedDoi":"10.21203/rs.3.rs-8310871/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8310871/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis study proposes an alternative to the template method for forming porous silica carriers. Bacterial cells of \u003cem\u003eRhodococcus fascians\u003c/em\u003e VKM B-1462 and \u003cem\u003ePseudomonas veronii\u003c/em\u003e VKM B-877 were utilized as biotemplates for the synthesis of a material based on tetraethoxysilane and methyltriethoxysilane (50/50 vol. %). As comparison samples, type III and IV materials were synthesized using cetyltrimethylammonium bromide (CTAB) micelles as templates, using methyltriethoxysilane and tetraethoxysilane 50/50 vol.%, as well as 100 vol.% tetraethoxysilane. The templates were removed from the materials by high-temperature annealing in the range from 200 to 1200 °C. The formation of a silicon-containing matrix was confirmed by scanning electron microscopy and IR spectroscopy. The optimal temperature for the removal of templates from materials was determined to be 800 °C for all types of material. An antiseptic substance, octenidine dihydrochloride, was loaded onto silicon carriers. UV spectroscopy revealed that the matrix formed using the \u003cem\u003eRhodococcus fascians\u003c/em\u003e biotemplate demonstrated optimal sorption and desorption properties. In comparison with the CTAB template, the employment of microbial cells has the potential to offer a number of applications due to cost-effectiveness, environmental safety, and ease of removal of the pore-forming agent.\u003c/p\u003e","manuscriptTitle":"Microbial biotemplates for obtaining silica porous carrier for antibacterial substance","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-01-12 11:21:15","doi":"10.21203/rs.3.rs-8310871/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2026-05-15T09:54:39+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-01-19T19:24:28+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"183010538393938778062776883164296073204","date":"2026-01-14T00:19:58+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2026-01-08T03:00:43+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-12-11T09:44:39+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-12-11T09:26:59+00:00","index":"","fulltext":""},{"type":"submitted","content":"Silicon","date":"2025-12-08T20:18:58+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"silicon","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scon","sideBox":"Learn more about [Silicon](https://www.springer.com/journal/12633)","snPcode":"12633","submissionUrl":"https://submission.nature.com/new-submission/12633/3","title":"Silicon","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"bef28b9e-0867-4be4-9295-f7669b63350d","owner":[],"postedDate":"January 12th, 2026","published":true,"recentEditorialEvents":[{"type":"decision","content":"Revision requested","date":"2026-05-15T09:54:39+00:00","index":"","fulltext":""}],"rejectedJournal":[],"revision":"","amendment":"","status":"in-revision","subjectAreas":[],"tags":[],"updatedAt":"2026-05-15T10:08:49+00:00","versionOfRecord":[],"versionCreatedAt":"2026-01-12 11:21:15","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8310871","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8310871","identity":"rs-8310871","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}