Anti-inflammatory potential of bromelain complexed with cashew leaf polyphenol

Anti-inflammatory potential of bromelain complexed with cashew leaf polyphenol | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Anti-inflammatory potential of bromelain complexed with cashew leaf polyphenol Reymark A. Placambo, Antonio Basilio, Ma. Cristina Las Llagas This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4676871/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Inflammation is a tissue reaction in which circulating immune cells and inflammatory mediators are sent to areas of infection and tissue injury. Chronic inflammation is treated using nonsteroidal anti-inflammatory medicines, which can have negative side effects on the body if used for an extended period. Hence, there is a need to develop effective and safe alternative anti-inflammatory compounds from natural sources to address this problem. Bromelain, a proteolytic enzyme derived from pineapple stems and fruits, has been extensively studied for its anti-inflammatory potential. This study determined the anti-inflammatory potentials of bromelain complexed with cashew leaf polyphenol (BCLP) from the bromelain recovered from pineapple core, pulp, and peel through the egg albumin denaturation assay, human red blood cell membrane stabilization assay, and human IL-8 secretion inhibition through ELISA. The results showed that the BCLP from pineapple core at 1000 µg/mL, 750 µg/mL, 250 µg/mL, and from 750 µg/mL pineapple pulp had a comparable inhibition of protein denaturation with the commercial bromelain at 1000 µg/mL, which may indicate that the BCLP from the pineapple core and pulp may be used as a potential anti-inflammatory compound alternative. Moreover, the anti-inflammatory activities of the BCLP from pineapple core, pulp, and peel were due to its complexation with CLP and were not caused by CLP alone. The discovery of the anti-inflammatory potentials of BCLP may elevate the value of pineapple fruit waste from waste products to being pharmaceutically relevant. Bromelain Bromelain complexed with cashew leaf polyphenol (BCLP) Cashew Leaf Polyphenols (CLP) Inflammation Egg albumin denaturation assay Human red blood cell membrane stabilization assay ELISA Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4676871","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":332929231,"identity":"ba9497f5-69a3-418e-ba48-ce0840c6f703","order_by":0,"name":"Reymark A. 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