Colchicine-Induced Polyploidy in pineapple (Ananas comosus L.) and Its Identification

Colchicine-Induced Polyploidy in pineapple (Ananas comosus L.) and Its Identification | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Colchicine-Induced Polyploidy in pineapple ( Ananas comosus L.) and Its Identification Junshuo Qiu, Yuanyuan Wang, Jing Zhang, Tao Li, Yuanrong Xu, Tingting Wang, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7507195/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 17 Nov, 2025 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted 4 You are reading this latest preprint version Abstract Enhancing the horticultural and commercial value of Ananas comosus through polyploidy has great potential, as does meeting market demands. This study used different colchicine treatments, durations, and concentrations to induce polyploidy in pineapple somatic embryo callus. Consequently, a maximum polyploid induction rate of 40.00% was achieved in somatic embryogenic callus exposed to 0.1% colchicine for 20 days. Flow cytometry revealed 202 tetraploids among the regenerated plants analyzed. Polyploid plants, in contrast to diploid ones, are morphologically characterized by having thicker, heavier, wider, and shorter leaves, a larger leaf area, a darker color, a shorter overall height, thicker stems, and fewer, shorter leaf spines that are more irregularly distributed and differently shaped. In polyploids, the stomata were both larger and less numerous than in diploid plants, and the stomatal guard cells and leaf epidermal cells were larger too. The study revealed that polyploid plants exhibited much higher levels of chlorophyll and PEPC enzyme activity than the diploid control plants. Compared to diploid control plants, polyploid plants displayed enhanced resistance to salt and heat following treatment. Overall, this research outlined an efficient strategy for inducing polyploidy in pineapples, paving the way for future advancements in pineapple germplasm resources. pineapple somatic embryogenesis polyploid plant tolerance tetraploidy Full Text Supplementary Files KeyMessage.pdf Supplementaryinformation.pdf Cite Share Download PDF Status: Published Journal Publication published 17 Nov, 2025 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted Reviewers agreed at journal 03 Sep, 2025 Reviewers invited by journal 03 Sep, 2025 Editor assigned by journal 03 Sep, 2025 First submitted to journal 01 Sep, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7507195","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":509880528,"identity":"7b26f21e-254e-4376-b811-4b91543ce8ee","order_by":0,"name":"Junshuo Qiu","email":"","orcid":"","institution":"Foshan University","correspondingAuthor":false,"prefix":"","firstName":"Junshuo","middleName":"","lastName":"Qiu","suffix":""},{"id":509880529,"identity":"bd88b526-7b44-496a-b270-27b1d38e6763","order_by":1,"name":"Yuanyuan Wang","email":"","orcid":"","institution":"Foshan 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