Study on the morphology of Mature ovarian follicles using Image Processing Toolbox

Abstract Objective: This study aims to investigate the morphological characteristics of mature follicles with clinical value. Study Design:a retrospective studyMethods: The five morphological indexes of follicles in 72 natural ovulation cycles were measured. The shoot time of follicle photos was the day before ovulation. Measurement software was the MATLAB Image Processing Toolbox. Results: The measured average area, perimeter, equivalent circle diameter and roundness were 301.29 ± 8.52 mm2, 62.23 ± 6.82 mm, 20.21 ± 3.08 mm and 0.89 ± 0.05, respectively; and the maximal diameter was 23.12 ± 3.06 mm. Conclusion: Image Processing Toolbox is a simple and convenient measurement software. The standardization of the reference indexes of follicular morphology has an important clinical application value in reproductive medicine.Research Highlights and Graphical Abstract: In the present study, the morphological characteristics of these folliclesinclude the area, perimeter, maximal diameter, equivalent circle diameter and roundness were preliminarily studied using the related measurement software.