Full-Length Transcriptome Sequencing and EST-SSR marker development of tiger lily (Lilium lancifolium Thunb.)

It is well known that transcriptional diversity plays important roles in plant biological regulation. But for the difficulty in full-length transcripts obtainment, the available tiger lily ( Lilium lancifolium Thunb ) transcriptome characterization are still not complete. To improve the integrity of tiger lily transcriptome information, (SMRT PacBio single-molecule long-read sequencing technology) was employed to accomplish the whole transcriptome profiling. A total of 815,624 CCS (Circular Consensus Sequence) reads with mean length of 1,295 bp were obtained. Based on these transcripts, 61,744 reads were full-length reads containing both the 5’ primer, 3’ primer and the poly (A) tail and 3,319 EST-derived SSRs were developed from 2968 unigenes. With the obtained informative reference transcriptome,768 transcription factors and 6,852 long non-coding RNAs were identified, providing a comprehensive framework of the transcriptional regulation network. Of all the annotated transcripts, 15,608 were distributed into 25 various Clusters of euKaryotic Orthologous Groups (KOG), and 10,706 unigenes were categorized into 52 functional groups which were divided into three categories. These results would provide a comprehensive set of reference transcripts and further improve our understanding of the tiger lily transcriptomes.