Phytochemical Analysis, In Vitro Antioxidant and Wound Healing Activities of Turbinaria ornata (Turner) J. Agardh from Gulf of Mannar, India

Abstract Turbinaria ornata, tropical brown algae found in the South pacific and Indian Ocean ecosystems. In accordance with recent studies, Turbinaria ornata J. has potent anti-inflammatory effects. Therefore, this study is aimed to explore the biological activities of ethanolic extract of Turbinaria ornata J by analyzing the presence of phytochemical components, antioxidant property, antimicrobial activity and the wound healing activity. From the results, phytochemical analysis of ethanolic extract of T. ornata showed the presence of alkaloids, saponins, oils, total phenolic and total flavanoid content were estimated to be 0.683 Abs and 0.433 Abs respectively. Anti-oxidant activity of the ethanolic extract of T. ornata extract showed remarkable DPPH radical scavenging activity of about 58.8% at 200μg/ml and total anti-oxidant activity of 0.257 absorbance at 100μg/ml concentration, as compared to that of their respective controls. The ethanolic extract of T. ornata exhibited the maximum zone of inhibition against the clinical pathogens like Pseudomonas aeuruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans and Methicillin-resistant Staphylococcus aureus with their potent anti-microbial activity. Wound healing effects of the ethanolic extract of T. ornata was analysed by using Zebra fish model. The results showed the rapidand significant regeneration of the wounded caudal fin on day 14. Therefore, the preliminary results of this study strongly supports the ethanolic extract of T. ornata may be effective in wound healing and regeneration of the wounded tissues.