Effect of All-Trans-Retinal on the Migration of Endometrial Stromal Cells and Expression of RDH10 mRNA in Endometriosis

Objective:To investigate the effect of all-trans-retinal(at-Ral)on the migration and retinol dehydrogenase(RDH10)mRNA expression of in vitro cultured human endometrial eutopic endometrial stromal cells.Methods:Endometrial stromal cells were isolated in vitroin normal women(N-ESC)and women with endometriosis(EMT-ESC).Then we observed the effects of different concentrations of at-Ral by cell scratch assay and real time PCR.Results:The migration distance and expression of RDH 10 mRNA of N-ESC decreased with the increased concentration of at-Ral.There were significant differences between 1,10μmol/L groups and the control group(P0.05),while the migration distance and expression of RDH 10 mRNA of EMT-ESC increased with the increased concentration of at-Ral.There were significant differences between 1,10μmol/L groups and the control group(P0.05).Conclusion:At-Ral can promote migration and expression of RDH10 mRNA of eutopic endometrial stromal cell of endometriosis.