Human in vitro endometriosis microphysiological system

Abstract Endometriosis is a debilitating condition that causes severe pain and infertility, among other morbidities. It affects 10% of reproductive-aged women worldwide and poses a significant public health challenge due to its prevalence and the lack of effective treatments and diagnostic tools. The study of endometriosis is restricted by the difficulty in modelling its multifactorial nature. To address this challenge, we developed a microphysiological system capable of reproducing key features of the menstrual cycle and endometriosis. In this publication, we describe such system and proof-of-concept data using simplified in vitro models that validate its use.