Effects of Jiawei Sanlengwan on estrogen secretion and angiogenesis of human endometrium of endometriosis

AIM To study the effect of Jiawei Sanlengwan(SLW) on the secretion level of estradiol(E2) and the expression and secretion level of vascular endothelial growth factor(VEGF) in endometrial cells of endometriosis,and then to approach the mechanism of inhibiting angiogenic potential of endometriosis.METHODS The eutopic endometrial cells of hysteromyoma and endometriosis patients were cultured,and the secretion level of E2 in endometrial cells in the culture media supernatant at different time point with the treatment of serum of rats ig SLW(5.0,2.5,1.25 g·kg-1·d-1,ig,bid × 7 d) was detected by electrochemilu-minescence immunoassay,then the expression and the secretion level of VEGF with the treatment of serum of rats ig SLW 2.5 g·kg-1·d-1 was analyzed by Western blot and ELISA,respectively.After the optimal time for SLW 2.5 g·kg-1·d-1 to inhibit the expression and secretion level of VEGF was identified based on the time-effect relationship,another endometrial cells were divided into six groups:hysteromyoma control group,endometriosis control group,endometriosis + SLW(2.5 g·kg-1·d-1) group,endometriosis + anastrozole group,endometriosis + SLW(2.5 g·kg-1·d-1) + E2 group,and endometriosis + E2 group.The expression and secretion level of VEGF in endometrial cells of each group was detected according to the optimal time point respectively.RESULTS The secretion level of E2 of eutopic endometria with endometriosis could be decreased by high dose and middle dose of SLW(5.0,1.25 g·kg-1·d-1),which showed the dependence of time and dose.The result of Western blot and ELISA indicated that the expression and the secretion level of VEGF protein of the endometriosis control group were significantly higher than that of the hysteromyoma control group at each time point(P 0.01).After the 24-hour treatment with serum of rats admistrated by SLW 2.5 g·kg-1·d-1,the expression and secretion level of VEGF protein was significantly lower than that of the endometriosis control group(P 0.01).After the 24-hour combined treatment of 1.34 ng·L-1 E2 and serum of rats admistrated by SLW 2.5 g·kg-1·d-1,the angiogenesis promoting effect of eutopic endometrial cells reversed by SLW could be partly or completely recovered by E2,and there was no statistical difference(P 0.05) in the secretion level of VEGF protein compared with the endometriosis control group.With the treatment of 1.34 ng·L-1 E2 for 24 h,the expression and secretion level of VEGF protein in eutopic endometrial cells was significantly higher than that of the endometriosis control group(P 0.01),but anastrozole 0.1 mg·kg-1·d-1 had the contrary effect to E2.CONCLUSION SLW could decrease the secretion of E2 of the eutopic endometrial cells so as to inhibit the expression and secretion of VEGF to play a role in inhibition of angiogenesis in the eutopic endometrial cells of endometriosis.