Ten-organ developmental proteome atlas from infancy to adulthood mice

Abstract The early-life organ development and maturation process shapes the fundamental blueprint for the later-life phenotype. However, the proteome atlas of self-multi-organs from infancy to adulthood is currently not available. Herein, we present a comprehensive proteomic analysis of ten mice organs (brain, heart, lung, liver, kidney, spleen, stomach, intestine, muscle and skin) acquired from the same individuals at three essential developmental stages (1-week, 4-week and 8-week after birth) by data-independent acquisition mass spectrometry. We identified and quantified 11,533 proteins in 10 organs and obtained 115 age-related differentially expressed proteins that were co-expressed in all organs from infancy to adulthood. We found that spliceosome proteins prevalently play essential regulatory functions in the early-life development of multiple organs, in the expression of unique organ properties, and in the sexual dimorphism of organs. This self-multi-organ proteome atlas provides a fundamental baseline for understanding the molecular mechanisms underlying organ development and maturation in early- life.