Another Feature of Silicon Nanowire Field Effect Transistor Biosensor: Dynamic Detection

Silicon nanowire field effect transistor (SiNW-FET) biosensors are capable of label-free, real-time and biological detection with high sensitivity and specificity. However, direct observation on protein-protein interaction in blood or serum is still very difficult because of the complex physiological environment and Debye-screening effect. In order to overcome the detection obstacles, we used dialysis desalination method to purify the detection fluid and overcome Debye-screening effect. In our research, a top-down approach was proposed to fabricate the SiNW-FET, APTES-Glu chemical chain was used to link antibody to the SiNWs. And after verified the detection ability of silicon nanometer biosensors, the dynamic detection process of HbA1c was successfully realized. This could be helpful for accurate diagnosis of diabetes for clinical application. And it makes it possible to dynamic research of small biomolecules without markers.