Optimization of Subcritical Water Pretreatment on Glutinous Rice Husk for Efficient Sugar and Hydrogen Production

Optimization of Subcritical Water Pretreatment on Glutinous Rice Husk for Efficient Sugar and Hydrogen Production | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Optimization of Subcritical Water Pretreatment on Glutinous Rice Husk for Efficient Sugar and Hydrogen Production Izzah Farhana Ab Aziz, Hasfalina Che Man, Anelka Dau, Norhashila Hashim, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4551137/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract There are thousands of different rice cultivars available worldwide, and glutinous rice ( Oryza sativa var. glutinosa ), sometimes known as sticky rice, is one of the most well-known varieties. Glutinous Rice Husk (GRH) is categorized as lignocellulosic biomass obtained as a by-product of rice manufacturing. The GRH is residue with a substantial amount of cellulose, hemicelluloses, and lignin, but it is difficult to degrade GRH during anaerobic digestion or fermentation. This research aims to investigate the factors affecting the sugar production of GRH by subcritical water (SCW) pretreatment. It is expected that the sugar produced could increase biohydrogen production. The biohydrogen yield was compared between raw and SCW-treated GRH as a fermentation substrate. Response Surface Methodology (RSM) was utilized to determine the ideal parameters for the subcritical water pretreatment of GRH using a Central Composite Design in the Design Expert Software. SCW pretreatment with temperature (195°C), solid-to-liquid ratio (1:10), and reaction time (17.5 minutes) were selected for the optimum sugar production (647.2 mg/L total sugar) of GRH. This pretreatment improves hydrogen production during dark fermentation by increasing the availability of easily accessible simple sugars for microbes. Additionally, it improves the lignocellulosic structure of GRH to enhance the fermentation process. Dark fermentation with SCW-treated GRH (0.73 mL H 2 /g-TS) obtained higher hydrogen yield than raw GRH (0.09 mL H 2 /g-TS). These findings highlight the potential of SCW pretreatment as an effective strategy for maximizing hydrogen production from GRH and contributing to using lignocellulosic biomass for renewable energy applications. glutinous rice husk (GRH) lignocellulosic biomass subcritical water pretreatment hydrolysis sugar production hydrogen Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4551137","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":314377706,"identity":"21d0f129-cdda-4ce5-9461-c842a3861c1f","order_by":0,"name":"Izzah Farhana Ab Aziz","email":"","orcid":"","institution":"Universiti Putra Malaysia Fakulti Kejuruteraan","correspondingAuthor":false,"prefix":"","firstName":"Izzah","middleName":"Farhana Ab","lastName":"Aziz","suffix":""},{"id":314377707,"identity":"cebecaf4-8ccd-4fe8-abd9-2dae3dfff49a","order_by":1,"name":"Hasfalina Che 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Glutinous Rice Husk (GRH) is categorized as lignocellulosic biomass obtained as a by-product of rice manufacturing. The GRH is residue with a substantial amount of cellulose, hemicelluloses, and lignin, but it is difficult to degrade GRH during anaerobic digestion or fermentation. This research aims to investigate the factors affecting the sugar production of GRH by subcritical water (SCW) pretreatment. It is expected that the sugar produced could increase biohydrogen production. The biohydrogen yield was compared between raw and SCW-treated GRH as a fermentation substrate. Response Surface Methodology (RSM) was utilized to determine the ideal parameters for the subcritical water pretreatment of GRH using a Central Composite Design in the Design Expert Software. SCW pretreatment with temperature (195\u0026deg;C), solid-to-liquid ratio (1:10), and reaction time (17.5 minutes) were selected for the optimum sugar production (647.2 mg/L total sugar) of GRH. This pretreatment improves hydrogen production during dark fermentation by increasing the availability of easily accessible simple sugars for microbes. Additionally, it improves the lignocellulosic structure of GRH to enhance the fermentation process. Dark fermentation with SCW-treated GRH (0.73 mL H\u003csub\u003e2\u003c/sub\u003e/g-TS) obtained higher hydrogen yield than raw GRH (0.09 mL H\u003csub\u003e2\u003c/sub\u003e/g-TS). 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