Phyllobacterium melilotisp. nov. a novel non-symbiotic bacterium isolated from root nodules ofMelilotus albus(white sweet clover) grown in Canada

Two novel bacterial strains isolated from root-nodules of white sweet clover ( Melilotus albus ) plants grown at a Canadian site were previously characterized and placed in the genus Phyllobacterium . Here we present phylogenomic and phenotypic data to support the description of strain T1293 T as representative of a novel species and present the first complete closed genome sequence of a bacterial strain (T1018) representing the species ‘ P. pellucidum’ . Phylogenetic analysis of genome sequences as well as analysis of 53 core genes placed novel strain T1293 T in a highly supported cluster of strains distinct from named Phyllobacterium species with P. myrsinacearum and P. calauticae as closest relatives. The highest average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of genome sequences of T1293 T compared to closest species type strains (84.1% and 26.5%, respectively) are well below the threshold values for bacterial species circumscription. The genome of strain T1293 T has a size of 5074034 bp with a DNA G+C content of 55 mol% and possesses three plasmids with sizes of 397619 bp, 476847 bp and 519835 bp. Detected in the genome were Type III and Type VI secretion system genes, implicated in plant-microbe and microbe-microbe interactions, but key nodulation, nitrogen-fixation and photosystem genes were not detected. Further analysis revealed that T1293 T , like other Phyllobacterium species, possesses key genes encoding an enzyme complex implicated in the degradation of glyphosate, a widely used broad-spectrum herbicide that has negative consequences for many microorganisms including the human gut microbiome. A novel prophage (size ∼ 41.5 kb) was also detected in the genome of T1293 T . Data for multiple phenotypic tests complemented the sequence-based characterization of strain T1293 T . The data presented support the description of a new species and the name Phyllobacterium meliloti sp. nov. is proposed with T1293 T = LMG32641 T = HAMBI 3765 T as the species type strain.