BIOLOGICAL ACTIVITY ASSESSMENT OF FUNCTIONALIZED SILVER NANOMATERIALS SYNTHESIZED FROM BLACK ROSEHIP (Rosa pimpinellifolia L.) SEEDS

Silver nanoparticles (AgNPs) have garnered significant attention due to their unique properties and wide range of applications. The green synthesis of AgNPs using plant extracts is an eco-friendly and cost-effective approach that has been extensively studied. This study presents the first-time synthesis and evaluation of silver nanoparticles (AgNPs) using black rosehip ( Rosa pimpinellifolia L.) seed aqueous extract as a potential pharmacological agent. The green synthesis approach yielded spherical Rp-AgNPs with dimensions between 5-200 nm and an average size of 51.93 nm, confirmed by various characterization techniques including UV-Vis spectroscopy, FTIR, TEM, SEM, XRD, DSC, Raman spectroscopy, and XPS analysis. The Rp-AgNPs demonstrated significant antioxidant, cytotoxic, and antimicrobial properties. Their antioxidant capacity surpassed that of the seed extract alone, with a lower IC 50 value in the DPPH assay. The nanoparticles exhibited cytotoxicity against H460 non-small cell lung cancer cells, with an IC 50 of 65.8 μg/mL. Antimicrobial activity was observed against both bacteria and fungi, with inhibition zones ranging from 10-14 mm for bacteria and 7-8 mm for Candida strains. Minimum Inhibitory Concentration values ranged from 50-100 μg/mL for bacteria and 100-200 μg/mL for Candida . This comprehensive study highlights the potential of black rosehip seed extract-mediated AgNPs for various pharmacological applications, especially antioxidant, anticancer and antimicrobial agents, paving the way for further exploration and development in the field of green nanotechnology for biomedical applications.