Primary vaginal adenocarcinoma arising in vaginal adenosis after CO2 laser vaporization and 5-fluorouracil therapy.

Formalin-fixed, paraffin-embedded tissue sections were used. Biotin-labeled DNA-specific HPV probe solutions for types 16 and 18 were applied to individual sections. Detection of hybridized probe was performed by tyramid-catalyzed signal amplification using the Dako Genpoint Kit (Dakocytomation, CA). Chromogenic detection was performed with DAB/H 2 O 2 . Controls included tissue sections positive for HPV wide spectrum, the HeLa cell line for HPV 18 and the SiHa cell line for HPV 16. Biotin-labeled plasmid probes served as a negative control in each case. Cases with a discrete punctate reaction product in the cell nuclei were interpreted as positive.

A 45-year-old White women with a longstanding history of abnormal Pap smears and cervical biopsies including high and low-grade cervical dysplasia, and positive testing for both high and low-risk HPV types, was referred to the gynecologic oncology service for follow-up and treatment. Her past medical history was unremarkable. Notably, there was no history of DES exposure or mullerian abnormalities. Despite treatment with laser conization and cryosurgery, she continued to have abnormal Pap smears, and she therefore underwent a hysterectomy in 2005 for definitive therapy. The patient has continued to have recurrent vaginal dysplasia since that time, with abnormal vaginal smears. She underwent a vaginal CO 2 laser vaporization 2 years after her hysterectomy, after which she continued to have abnormal vaginal smears ranging from low to high-grade dysplasia. The patient was treated with 5-FU cream on and off for approximately 1 year, alternating with estrogen hormonal therapy. The patient then had a second vaginal laser vaporization, at which time an area with red granulation tissue was biopsied. After the biopsy diagnosis, the patient underwent a radical upper vaginectomy, bilateral pelvic lymph node dissection and bilateral salpingo-oophorectomy.

The presence of vaginal adenosis is most commonly associated with DES exposure in utero , but can also be seen in cases of mullerian developmental disorders. In a series of autopsies examined by Sandberg ( 6 ), occult vaginal adenosis was found in 41% of postpubertal patients and in 0% of prepubertal patients, suggesting that adenosis develops de novo after puberty rather than during vaginal embryogenesis. More recently, there have been reports of vaginal adenosis after different types of vaginal trauma such as the application of 5-FU ( 1 – 3 ), CO 2 laser vaporization ( 4 ), Stevens-Johnson syndrome ( 7 ) and contact irritation from a disinfectant used preoperatively ( 8 ). Although the pathogenesis of vaginal adenosis is still not completely understood, in cases of adenosis after vaginal trauma or irritation, it is suggested that adenosis develops as a metaplastic process similar to other well-known types of metaplasia that occur as a result of noxious stimuli in other parts of the body. Vaginal adenocarcinomas are extremely rare, most commonly being clear cell carcinomas associated with in utero DES exposure ( 9 ). In nonexposed individuals, they are believed to arise from vaginal adenosis, endometriosis, or, rarely, Gartner’s duct remnants ( 10 ). To our knowledge, there has only been one earlier report in the literature of a vaginal adenocarcinoma arising in vaginal adenosis after 5-FU therapy. This case was a clear cell type diagnosed after treatment for condyloma acuminatum. The relationship between HPV infection and genital neoplasia has been well established ( 11 ). There has been one other case reported in the literature showing HPV DNA in clear cell adenocarcinoma of the vagina ( 12 ). Our patient presented with well-differentiated endocervical-type adenocarcinoma, in a background of vaginal adenosis, following a long history of HPV infection and trauma secondary to multiple laser ablation procedures and prolonged 5-FU therapy. As there was no earlier biopsy to document the presence of adenosis, we could not be certain as to the lapse of time of progression from adenosis to carcinoma. In our case, the vaginal adenosis could be the result of numerous factors: (1) metaplastic process resulting from multiple traumas and injuries caused by 5FU and laser vaporization; (2) because occult adenosis is observed in postpuberal women, 5FU could unmask an occult adenosis and even stimulate its extension; (3) as HPV is well known to be a cancer initiating agents, a direct involvement of HPV in the malignant transformation of vaginal adenosis is also a plausible possibility; and (4) simply the combination of all these factors. In conclusion, we report a case of well-differentiated endocervical-type adenocarcinoma of the vagina, arising in a background of vaginal adenosis after treatment of persistent VAIN-III with 5-FU and CO 2 laser vaporization. This case supports the theory that vaginal adenosis can develop as a metaplastic process after trauma and not just embryologically. The finding of HPV DNA in the glandular epithelium suggests that HPV could be involved in the malignant transformation of vaginal adenosis to invasive adenocarcinoma. However, this issue is still open to discussion and speculation and more efforts are required to address it.

The vaginal biopsy revealed well-differentiated endocervical-type adenocarcinoma, measuring 0.3 × 0.2 cm associated with high-grade VAIN and extending to the resection biopsy site ( Figs. 1A–C ). Immunohistochemisty revealed neoplastic glands to be positive for AE1/3, CEA-m and negative for vimentin, and ER to be consistent with endocervical-type adenocarcinoma. In situ hybridization for highrisk HPV types 16 and 18 were positive in both squamous and glandular components ( Fig. 2 ). The earlier hysterectomy specimen slides were reviewed and we confirmed the presence of low-grade cervical dysplasia and that the cervix was removed in its entirety. The upper vaginectomy specimen showed VAIN III of the anterior vagina associated with invasive, well-differentiated adenocarcinoma (CEA-positive) ( Figs. 3A, B ) arising in a background of vaginal adenosis (CEA-negative) ( Figs. 4A, B ). The margins were free of tumor and there was no evidence of metastatic disease. The patient did not have any postoperative complications. No further therapy was given and follow-up at 2 to 3-month intervals is planned.