Routine Histopathological Evaluation After Appendectomy: Is It Necessary? A Systematic Review.

Acute appendicitis is referred to as inflammation of the inner lining of the appendix, which then may advance to other parts of the organ and surrounding areas. Obstruction of the lumen is one of the major causative factors behind this pathology which can be due to a variety of reasons. Fecaliths and lymphoid hyperplasia are the usual etiology of this clinico-pathological condition, however, other unusual causes can also not be neglected which can be benign neuroma, mucocele, mucinous cystadenoma, endometriosis, gastrointestinal stromal tumor, carcinoid tumors of the appendix, hyperplastic polyp, lymphoma, leukemia, diverticulitis, granulomatous diseases, or infections say amebiasis, enterobiasis, actinomycosis, balantidiasis, schistosomiasis, trichuriasis, or tuberculosis (TB) [ 1 ]. Acute appendicitis is one of the most common reasons for acute abdominal pain. Though it can occur in any age group, it is quite frequently seen between the ages of 10 and 20 years. The lifetime risk for males is 8.6% and for females is 6.7% in the United States [ 2 ]. The clinical features which primarily put suspicion at diagnosis of acute appendicitis in adults are right lower quadrant pain, abdominal rigidity, and radiation of periumbilical pain to the right lower quadrant. In children, absent or decreased bowel sounds, a positive Psoas sign (pain on passive extension of the right thigh), a positive Rovsing sign (palpation of the left lower quadrant of abdomen intensifies the pain felt in the right lower quadrant), positive Obturator sign (pain on passive internal rotation of the flexed thigh) help in ruling in the diagnosis in favor of acute appendicitis [ 3 ]. Surgical appendectomy is the mainstay treatment of acute appendicitis which can be performed either as an open surgery or laparoscopic (minimally invasive) procedure. A recent meta-analysis of randomized controlled trials comparing postoperative results of laparoscopic and open surgery showed that there is lesser postoperative complications, lower incidence of wound infection, shorter duration of stay, and a comparatively faster return to normal activity in favor of laparoscopic operation and a smaller operation time in favor of open surgery via a limited right lower quadrant incision [ 4 ]. The protocol of sending appendectomy specimens for histopathological examination (HPE) is variable in various hospitals. This article presents a systematic review from various studies to provide a better basis for assessing the frequency of unusual findings in appendectomy specimens and whether a routine HPE of appendectomy specimens is needed.

Materials and methods On the basis of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines [ 5 ], a thorough literature search was conducted using PubMed, PubMed Central (PMC), and Google Scholar for the relevant published studies using the following keywords both alone and in combination: “Acute appendicitis” and “routine histopathological examination”. Inclusion/Exclusion Criteria Papers from 2000 till June 2020 in the English language were screened. Research papers in other languages and duplicate papers were not included. Only peer-reviewed articles were included and grey literature was not included. PRISMA diagram depicting the selection and screening of records is shown in Figure 1 . Results After applying the above-mentioned search criteria, 15 articles were selected for the final review that collectively had 57,524 cases. All these studies included in this systematic review are peer-reviewed. Details of the studies and relevant related details are summarized in Table 1 . HPE: Histopathological examination Discussion The appendix is a blind-ended tube that generally sits in the lower right quadrant of the abdomen. Earlier, the appendix has been considered as a rudimentary organ and labeled “vestigial organ” (organ which has lost its function along the evolutionary pathway) until recently when a significant number of studies have shown its immunoprotective role in the intestinal immune system [ 20 ]. Pathophysiology of Acute Appendicitis The main underlying etiology of acute appendicitis is luminal obstruction of the appendix which may be due to lymphatic hyperplasia resulting in increased intraluminal pressure leading to edema, ulceration, and inflammation which also increases its susceptibility to bacterial infections. The usual causes of acute appendicitis include fecalith and lymphatic hyperplasia. The unusual causes of appendicitis can be - Infectious or Lesions (Benign/Malignant). The unusual infectious causes of appendicitis can be due to ascariasis, amebiasis, enterobiasis, actinomycosis, balantidiasis, tuberculosis, trichuriasis, or schistosomiasis [ 1 ]. Lesions such as benign neuroma, mucocele, mucinous cystadenoma, endometriosis, gastrointestinal stromal tumor, carcinoid tumors of the appendix, hyperplastic polyp, lymphoma, leukemia, granulomatous diseases, or leiomyoma can also be unusual causes of appendicitis [ 1 ]. Diagnosis and Management Alvarado score is a clinical scoring system that takes into account six clinical features and two laboratory findings. It is a useful diagnostic score to rule out other diagnosis, however, its efficacy is inconsistent in children and overpredicts the chance of appendicitis in females. The score of 7-10 is highly suggestive and needs surgery. A score of 5-6 should not be discharged but kept in observation, however, a score of 1-4 can be discharged on the doctor’s discretion [ 21 ]. Table 2 depicts the scoring criteria of Alvarado score. Role of Routine Histopathological Examination of Surgical Specimens Routine HPE of a surgical specimen is generally considered beneficial as it helps to find out any unusual findings. However, due to the substantial costs of lab tests and limited resources available to the hospital, the efficacy and positive impact on the management needs to be significant to encourage routine pathological examinations. A recent meta-analysis involving analysis of 4012 cases of acute appendicitis clearly stated that though the patients with unusual pathological findings are less (6.4%) it is still a significant value and impacts not only the prognosis but also the management of the patient. The whole treatment plan may need to be changed in case of unusual findings and therefore, clearly recommended routine HPE for all appendectomy specimens [ 6 ]. Another retrospective study involving systematic review and meta-analysis of 24,697 cases found the incidence of neoplastic lesions at 0.7% and unusual pathological diagnosis in 0.9%. Neoplastic lesions seen were carcinoid tumors, mucinous neoplasms, and adenocarcinoma. It concluded that though routine HPE is expensive but it is still recommended to continue doing routine histology because of a significant number of unusual diagnosis [ 11 ]. A clinical audit of a hospital in the United Kingdom on meta-analysis showed that about 1.7% of the total cases had unusual pathology other than inflammation of the appendix. These abnormal pathologies resulted in the change of the management plan of the patients. It justified doing routine HPE as there was a significant change in the management plan if unusual findings were seen [ 9 ]. To summarize, for a better holistic approach, several recent retrospective studies and meta-analysis have favored and recommended the policy of doing routine HPE of appendectomy specimens to rule out unusual pathologies. Limitations Even after all the accessible records available, our systematic review has some limitations. We only included literature available in the English language and excluded some abstracts for which we were unable to retrieve the full text. Non peer-reviewed articles were not included.

Acute appendicitis is not caused only due to a single etiology but can occur due to a variety of causes which may be parasitic infections, benign or malignant lesions. On finding unusual etiology on histopathological examination of appendectomy specimen, it usually results in changing the management plan of the patient. There is sufficient evidence suggesting various uncommon etiologies that may present as acute appendicitis in several cases. Though lab costs may be substantial and unusual findings are only seldom seen but it is still recommended to perform a routine histopathological examination of all appendectomy specimens to rule out unusual pathologies.