The application of induced pluripotent stem cells to assess the toxic effects of environmental contaminants.

The application of induced pluripotent stem cells to assess the toxic effects of environmental contaminants. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The application of induced pluripotent stem cells to assess the toxic effects of environmental contaminants. Juned Kadiwala, Corina Ciocan, Rameen Shakur This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4642469/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Human exposure to environmental contaminants is inevitable due to lifestyle, geographical proximity to the source, socioeconomic status, and the omnipresence of pollutants. The growing body of literature has advanced our understanding of the potential toxicity of a wide range of contaminants, from pharmaceuticals to nano plastics, however, knowledge gaps still exist. Homosalate (HS) and Triphenyl Phosphate (TPP) are chemical compounds of different commercial products used extensively by consumers. Both chemicals have been detected in the human bloodstream, raising concerns about the potential adverse effects on the cardiovascular system and human health. In this study, we harness the potential of human induced pluripotent stem cell-derived cardiomyocytes (hiPSCs-CMs) as a model to test the effect of these two chemicals on cardiac gene expression profiles, using the quantitative PCR method. Our findings showed that continuous exposure to HS and TPP at 30 and 60 µM doses for 24 hours leads to a dose-dependent significant reduction in the expression of the key cardiac structural and functional genes (TNNT2, TNNC1, MYH-6, MYH-7, MYL-2, ACTN2, and MYBPC3). Thus, this study demonstrated the feasibility of using hiPSCs-CMs as an in vitro model to access cardiotoxic and other functional parameters. Full Text Supplementary Files ESM1.doc ESM2.doc ESM3.doc Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4642469","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":323854083,"identity":"d74ce6dc-ebea-4fd1-b751-edc9924fdac0","order_by":0,"name":"Juned Kadiwala","email":"","orcid":"","institution":"University of Brighton School of Applied Sciences","correspondingAuthor":false,"prefix":"","firstName":"Juned","middleName":"","lastName":"Kadiwala","suffix":""},{"id":323854084,"identity":"84edabc9-dc4d-4724-98a4-08bb70a3d7e1","order_by":1,"name":"Corina 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