Identification and Expression Analysis of the Magnolia officinalis AP2/ERF Gene Family Based on Different Age Groups

Identification and Expression Analysis of the Magnolia officinalis AP2/ERF Gene Family Based on Different Age Groups | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Identification and Expression Analysis of the Magnolia officinalis AP2/ERF Gene Family Based on Different Age Groups Hui Tian, Qian Wang, MingXin Zhong, Yuanyuan Zhang, Bainian Zhang, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4565493/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective The purpose of this study was to identify the AP2/ERF gene family in Magnolia officinalis (M. officinalis) and to examine changes in AP2/ERF gene expression and functional annotation as M. officinalis grows. Methods The transcriptome data of M. officinalis were evaluated and compared to those of the model plant Arabidopsis thaliana . After screening, 75 distinct AP2/ERF gene family sequences (Ho AP2/ERF 1 ~ Ho AP2/ERF 75) were identified and analyzed via bioinformatics methods. The expression levels of XR and DR in the two groups were determined using Heatmap Tree, and genes with substantial expression were examined and functionally annotated. Results The 75 Ho AP2/ERF proteins all had one or more conserved AP2 domains. Its protein stability is usually indicative of an unstable protein. Ho AP2/ERF family proteins are hydrophilic. Subcellular research revealed that it was primarily located in the nucleus. The expression levels of certain AP2/ERF genes differed considerably across the XR and DR groups, with XR being overexpressed compared to those in the DR group. Conclusion M. officinalis' AP2/ERF gene family is divided into four subfamilies. Its role not only is related to M. officinalis growth and development, as well as secondary metabolism but also affects its stress tolerance. Furthermore, the low-aged group of M. officinalis XR exhibited greater stress tolerance than did the high-aged group of M. officinalis DR. Among the significantly expressed genes, Ho AP2/ERF 55 controls the activity of imidazole glycerol phosphate synthase, while Ho AP2/ERF 39 plays a role in plant–pathogen interactions by regulating the expression of PR genes. Magnolia officinalis AP2/ERF transcription factor Bioinformatics Resistance against the environment Full Text Additional Declarations No competing interests reported. Supplementary Files ManuscriptData1.txt Magnolia officinalis AP2/ERF sequences Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4565493","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":317515168,"identity":"a1965d0a-e59f-4169-acf7-e6049ca76cb0","order_by":0,"name":"Hui Tian","email":"","orcid":"","institution":"Mianyang Normal University","correspondingAuthor":false,"prefix":"","firstName":"Hui","middleName":"","lastName":"Tian","suffix":""},{"id":317515169,"identity":"bbc5d825-bcc1-4251-b916-e6a088e3885d","order_by":1,"name":"Qian Wang","email":"","orcid":"","institution":"Mianyang Normal 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Ltd","correspondingAuthor":false,"prefix":"","firstName":"Zhenpeng","middleName":"","lastName":"Xi","suffix":""}],"badges":[],"createdAt":"2024-06-11 16:24:13","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4565493/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4565493/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":67779578,"identity":"94bcb048-6491-44a6-97a3-5eb73da891a8","added_by":"auto","created_at":"2024-10-29 15:39:14","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1330080,"visible":true,"origin":"","legend":"","description":"","filename":"reviewedCleanManuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4565493/v1_covered_e6759139-913c-4c97-867f-8637976d8bf7.pdf"},{"id":59295071,"identity":"5bd3c549-3211-4281-804c-a2b94ec9fa4d","added_by":"auto","created_at":"2024-06-28 19:50:31","extension":"txt","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":22994,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cem\u003eMagnolia\u003c/em\u003e officinalis \u003cem\u003eAP2/ERF\u003c/em\u003e sequences\u003c/p\u003e","description":"","filename":"ManuscriptData1.txt","url":"https://assets-eu.researchsquare.com/files/rs-4565493/v1/d41c051eca48edd1d9f30569.txt"}],"financialInterests":"No competing interests reported.","formattedTitle":"Identification and Expression Analysis of the Magnolia officinalis AP2/ERF Gene Family Based on Different Age Groups","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Magnolia officinalis, AP2/ERF transcription factor, Bioinformatics, Resistance against the environment","lastPublishedDoi":"10.21203/rs.3.rs-4565493/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4565493/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eObjective The purpose of this study was to identify the AP2/ERF gene family in Magnolia officinalis (M. officinalis)\u003c/h2\u003e \u003cp\u003eand to examine changes in AP2/ERF gene expression and functional annotation as M. officinalis grows. \u003cb\u003eMethods\u003c/b\u003e The transcriptome data of M. officinalis were evaluated and compared to those of the model plant \u003cem\u003eArabidopsis thaliana\u003c/em\u003e. After screening, 75 distinct \u003cem\u003eAP2/ERF\u003c/em\u003e gene family sequences (Ho\u003cem\u003eAP2/ERF\u003c/em\u003e1\u0026thinsp;~\u0026thinsp;Ho\u003cem\u003eAP2/ERF\u003c/em\u003e75) were identified and analyzed via bioinformatics methods. The expression levels of XR and DR in the two groups were determined using Heatmap Tree, and genes with substantial expression were examined and functionally annotated. \u003cb\u003eResults\u003c/b\u003e The 75 Ho\u003cem\u003eAP2/ERF\u003c/em\u003e proteins all had one or more conserved AP2 domains. Its protein stability is usually indicative of an unstable protein. Ho\u003cem\u003eAP2/ERF\u003c/em\u003e family proteins are hydrophilic. Subcellular research revealed that it was primarily located in the nucleus. The expression levels of certain \u003cem\u003eAP2/ERF\u003c/em\u003e genes differed considerably across the XR and DR groups, with XR being overexpressed compared to those in the DR group. \u003cb\u003eConclusion\u003c/b\u003e M. officinalis' \u003cem\u003eAP2/ERF\u003c/em\u003e gene family is divided into four subfamilies. Its role not only is related to M. officinalis growth and development, as well as secondary metabolism but also affects its stress tolerance. Furthermore, the low-aged group of M. officinalis XR exhibited greater stress tolerance than did the high-aged group of M. officinalis DR. Among the significantly expressed genes, Ho\u003cem\u003eAP2/ERF\u003c/em\u003e55 controls the activity of imidazole glycerol phosphate synthase, while Ho\u003cem\u003eAP2/ERF\u003c/em\u003e39 plays a role in plant\u0026ndash;pathogen interactions by regulating the expression of PR genes.\u003c/p\u003e","manuscriptTitle":"Identification and Expression Analysis of the Magnolia officinalis AP2/ERF Gene Family Based on Different Age Groups","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-06-28 19:50:27","doi":"10.21203/rs.3.rs-4565493/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"3f3fae8e-48b3-4885-a94a-ba61cc9add21","owner":[],"postedDate":"June 28th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-10-29T15:38:47+00:00","versionOfRecord":[],"versionCreatedAt":"2024-06-28 19:50:27","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4565493","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4565493","identity":"rs-4565493","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}