Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani
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CC-BY-NC-ND-4.0
Abstract
This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani . A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [ 3 H]-spermidine in L. donovani at a relevant IC 50 of 60 µM.
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Source provenance
- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-22T02:00:06.705733+00:00
License: CC-BY-NC-ND-4.0