Serum RANKL, OPG and Vitamin D in children with systemic lupus erythematosus

Serum RANKL, OPG and Vitamin D in children with systemic lupus erythematosus | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research article Serum RANKL, OPG and Vitamin D in children with systemic lupus erythematosus Sheng Hao, Jing Zhang, Bingxue Huang, Dan Feng, Xiaoling Niu, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1331100/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 7 You are reading this latest preprint version Abstract Indroduction: SLE is an autoimmune multisystem disease. Glucocorticoid is an irreplaceable medication for SLE. Glucocorticoid and inflammation impact bone remodeling by OPG/RANKL/RANK signal system, which could lead to osteoporosis. Our aim is to clarify the expression of RANKL/OPG in children with SLE, and to preliminarily explore the changes of bone metabolism related indexes in children with SLE. Methods: Serum RANKL and OPG of 40 children with SLE and healthy children were detected by ELISA, while 25(OH)VitD 3 was detected routinely. Clinical data of children with SLE were recorded, including gender, age, height, weight, BMI, duration of the disease, accumulated dose of glucocorticoid, and correlation analysis was conducted with RANKL, OPG and 25(OH)VitD 3 . Results: Serum RANKL concentration in SLE group was significantly higher than health group (9.82±7.20 vs. 6.80±4.35 pg/ml, P<0.05), and the concentrations of OPG and 25(OH)VitD 3 in serum were significantly lower than health group (156.34±57.33 vs. 189.16±68.70 pg/ml and 43.66±31.27 vs. 59.04±21.56 mmol/L, P<0.05). Serum RANKL in children with SLE was positively correlated with the duration of SLE, accumulated dose of GC(r=0.593, 0.727, P<0.05). And it was negatively correlated with serum OPG and 25(OH)VitD 3 (r=-0.601, -0.469, P<0.05). In addition, serum OPG and 25(OH)VitD 3 concentrations were inversely correlated with accumulated dose of GC (r=-0.66, -0.508, P<0.05). Conclusion: Low levels of vitamin D3 and bone metabolic abnormalities were found in children with SLE under the condition of disease remission, while serum RANKL expression was elevated, OPG expression was reduced. These changes associated with the duration of SLE, accumulated dose of GC. In the case of exclusion of disease activity, GC and other factors may be involved in the occurrence and development of abnormal bone metabolism through RANKL/OPG. Pediatric systemic lupus erythematosus Receptor activator of nuclear factor-κB ligand Osteoprotegerin Vitamin D Glucocorticoid Figures Figure 1 Indroduction Systemic lupus erythematosus (SLE) is an autoimmune multisystem disease with high disability rate and mortality. Glucocorticoid-induced osteoporosis (GIOP) is one of the most common complications of SLE[ 1 ]. Bone remodeling is a dynamic equilibrium biological process of the interaction between Osteoblast (OB) and Osteoclast (OC). Receptor activator of nuclear factor-κB ligand (RANKL) and Osteoprotegerin (OPG) act as a pair of biologic active proteins, which bind to their common receptor activator of nuclear factor-κB (RANK) to regulate the balance process of bone remodeling. Glucocorticoid(GC) and inflammation impact bone remodeling by OPG/RANKL/RANK signal system[ 2 ], which could lead to osteoporosis. In order to define and verify the SLE patients in the presence of RANKL/OPG expression changes and bone metabolic abnormalities, we detected RANKL, OPG and 25(OH)VitD 3 in 40 children with SLE in this study, while correlation between RANKL and other items were analysed, to discuss bone metabolism related changes in SLE condition. Participants And Methods Patients and controls 40 children with SLE were enrolled, who were treated and followed up in Shanghai Children's Hospital from January 2019 to July 2020, including 5 males and 35 females. All patients were diagnosed according to 2012 Systemic Lupus International Collaborating(SLICC) classification criteria[ 3 ]. Systemic lupus erythematosus disease activity index(SLEDAI) scores[ 4 ] of the patients were below 4, which means the disease were in remission. The control group included 40 healthy children who underwent physical examination at the same period, and the age and sex matched. Patients with early onset of lupus-like syndrome due to genetic mutation, SLEDAI scores greater than 4, and severe infection were excluded. The study was approved by the Ethics Committee of Shanghai Children’s Hospital(No. 2020R015), and the informed consent was signed by the patient’s parents. This study was performed in accordance with the Helsinki Declaration of 1964 and its later amendments. Experimental detection methods The whole blood samples of 40 children with SLE and healthy control group were collected and agglutinated for 30 minutes, then centrifuged at 1000 RPM for 10 min. Serum samples were stored at -80℃. RANKL and OPG were detected by Human Trance/TNFSF11/RANKL ELISA Kit and Human Osteoprotegerin/OPG ELISA Kit(Multisciences Biotech, Hangzhou, China). Serum 25(OH)VitD 3 was detected by Roche cobas E601 Analyzer. Statistical analysis SPSS 25.0 statistical software package was used for statistics. Serum RANKL, OPG, 25(OH) VitD 3 concentrations, age, duration of SLE, height, weight and accumulated dose of GC were expressed as mean ± standard deviation(SD). The comparisons between two groups were used by t-tests, and Pearson analysis was used to analyze the correlation between two indicators in children with SLE. Results General clinical data In this study, there were 40 children in the SLE group and 40 children in the normal control group, and there was no statistical difference in gender composition and age between the two groups (Table 1 ). Table 1 General clinical data of children with SLE group and control group (n or mean ± SD) SLE group (n = 40) Control group (n = 40) statistics P value Sex(male/female) 5/35 6/34 χ2 = 0.3922 0.5312 Age(years) 10.75 ± 2.56 11.4 ± 1.53 t = 1.378 0.1721 Serum RANKL, OPG and 25(OH) VitD3 concentrations in the SLE group Serum RANKL concentration in SLE group was significantly higher than that in normal control group (*P < 0.05), and the concentrations of OPG and 25(OH) VitD3 in serum were significantly lower than those in normal control group (*P < 0.05), the difference was statistically significant(Table 2 , Fig. 1 ). Table 2 Serum concentrations of RANKL, OPG and 25(OH) vitD 3 in SLE and control group (n or mean ± SD) SLE group(n = 40) Control group (n = 40) Statistics P value RANKL(pg/ml) 9.82 ± 7.20 6.80 ± 4.35 t = 2.276 0.0256 * OPG(pg/ml) 156.34 ± 57.33 189.16 ± 68.70 t = 2.319 0.0230 * 25(OH)vitD 3 (mmol/L) 43.66 ± 31.27 59.04 ± 21.56 t = 2.712 0.0082 * * P < 0.05 Correlation analysis of serum RANKL level and other indicators in SLE group Serum RANKL concentration in children with SLE was correlated with other indexes, including age, course of disease, weight, height, Body mass index (BMI), GC accumulation, OPG and 25(OH) VitD 3 . The results showed that in children with SLE serum RANKL was positively correlated with the duration of SLE and the accumulated dose of GC (*P < 0.05), and was negatively correlated with serum OPG and 25(OH) VitD3 (*P 0.05). In addition, serum OPG and 25(OH) VitD3 concentrations were inversely correlated with GC cumulants (*P < 0.05), the difference was statistically significant(Table 3 ). Table 3 Correlation analysis of serum RANKL level and other indexes in children with SLE(n or mean ± SD SLE group(n = 40) R值 P值 Age(years) 10.75 ± 2.56 -0.105 0.519 Duration of SLE(Months) 14.95 ± 4.94 0.593 < 0.0001 * Weight(kg) 42.33 ± 9.68 -0.279 0.081 Height(cm) 131.33 ± 13.92 -0.141 0.385 BMI(kg/m 2 ) 24.64 ± 4.88 -0.176 0.278 Accumulated dose of GC (mg/kg) 292.33 ± 67.93 0.727 < 0.0001 * RANKL(pg/ml) 9.82 ± 7.20 - - OPG(pg/ml) 156.34 ± 57.33 -0.601/-0.66 ** < 0.0001 * 25(OH)vitD 3 (mmol/L) 43.66 ± 31.27 -0.469/-0.508 ** 0.002 * /-0.001 * *P < 0.05, the difference was statistically significant ** The correlation with accumulated dose of GC Discussion Bone is a constantly metabolizing organ, and bone remodeling is a dynamic balance process of bone resorption and bone formation. OC absorbs old bone and Osteoblast OB forms new bone, respectively. Bone remodeling relies on the precise coordination of the two kinds of cells to keep in balance. OC can regulate the functions of OB in both positive and negative ways, while OB can adjust OC through OPG/RANKL/RANK signaling system. RANKL and OPG are a pair of important bioactive proteins in regulating the balance of bone metabolism, which are widely expressed in lymphoid tissues. RANKL is a member of tumor necrosis factor(TNF) superfamily, which activates downstream related molecules after binding to its receptor RANK to enhance osteoclast differentiation and activity, then leads to increased bone resorption. RANKL is highly expressed in the thymus gland, lungs and lymph nodes, and low expressed in the spleen and bone marrow[ 5 ]. A variety of cytokines, hormones, growth factors can regulate the expression of RANKL. RANK and OPG are also members of TNF Receptor superfamily. RANKL binds to RANK on the surface of osteoclasts to induce the accumulation of TNF receptor-related factor (TRAF6) in osteoclasts and promotes the differentiation and activation of osteoclasts, while the apoptosis of osteoclasts was inhibited. OPG has two forms including monomer and dimer, and is mainly expressed in osteoblasts and vascular cells[ 6 ]. It can competitively bind RANK, so the ratio of RANKL/OPG plays a key role in the process of bone remodeling. Our study mainly detected serum RANKL and OPG in children with SLE. The expression of RANKL, OPG and RANK is regulated by different hormones and cytokines, including Parathormone(PTH), 1,25(OH) 2 VitD 3 , prostaglandins, TNF-α, Interleukin (IL)-6 and M-CSF, etc. The high expression of estrogen could increase the expressions of transforming growth factor (TGF) -β and OPG, which inhibited RANKL signal transduction, and then promoted bone formation. On the contrary, the low expression of estrogen could significantly increase the expressions of IL-1, IL-6, TNF-α and other pro-inflammatory factors, which increased the expression of RANKL and promoted bone resorption[ 2 ]. At present, the most commonly used method for bone mineral density(BMD) assessment is dual energy x-ray absorptiometry (DXA). But local DXA is insufficient to reflect the condition, and the whole body DXA is difficult to perform in children because of the high dose of radiation and coordination problem. PTH, 25 (OH)VitD 3 and osteocalcin and can only represent the body endocrine levels, so that new and simple biomarkers are needed, which are the reflection of bone metabolic state. Fracture risk assessment should be performed on all patients with long-term oral GC and should also be regularly assessed during treatment[ 7 ]. RANKL and OPG play a key role in the balance of osteoblasts and osteoclasts, which are very important in the regulation of bone metabolism in vivo and believed to be related to the reduction of bone mineral density in patients with SLE. A number of studies have shown that the RANKL/OPG ratio is decreased in chronic diseases such as type 1 diabetes, juvenile idiopathic arthritis, and nephrotic syndrome[ 8 , 9 , 10 , 11 ], which may represent the negative balance of bone remodeling. Therefore, the detection of RANKL and OPG may become new sensitive biomarkers to evaluate bone metabolism in children. It is reported that annual incidence of SLE in adults ranges from 0.3 to 31.5/100,000[ 3 , 4 ], and 30 to 70/100,000 in China[ 12 ]. The incidence in children ranges from 0.36 to 2.50/ 100,000, and the prevalence ranges from 1.89 to 25.70/100,000[ 13 ]. Although the incidence of SLE in children is not high, the vast majority of children with SLE need lifelong treatment, and the mortality is still high in young patients[ 14 ]. GC is widely used in patients with inflammatory, autoimmune and allergic diseases, and is one of the most common and irreplaceable drugs for SLE at present. With the application of GC and other immunosuppressants, the mortality of SLE patients has decreased significantly in recent decades. More than 80% of patients with SLE need long-term duration[ 15 , 16 ] so that GIOP is more common. GC stimulates RANKL and inhibit OPG, which promotes osteoclast differentiation and osteolysis, while it induces osteocyte apoptosis and inhibits osteocyte generation[ 17 ]. Studies have shown that bone loss caused by the GC can be roughly divided into two stages, bone mineral density loss fast which is about 6 ~ 12% in the first year of treatment, and about 3% a year later[ 18 ]. At the later stages, GC increased apoptosis of osteoblasts and osteocytes, and then the expression of RANKL decreased[ 19 ], while the number of osteoclasts decreased by apoptosis and autophagy. In addition, control of inflammation and reduction of GC dosage at the later stage also resulted in less bone loss. A study found that OPG, RANKL and RANKL/OPG were significantly increased in the SLE group, and OPG level was related to the activity of the disease[ 20 ]. Studies on children with SLE also showed that RANKL level was not related to disease activity[ 21 ]. Therefore our study selected the patients with disease activity controlled to reduce the interference of inflammation. The results showed that serum RANKL and OPG concentrations in inactive state of disease are also significantly different with normal children. It suggested that dynamic balance regulation of bone metabolism still exists in the remission state of the disease, partially verifying the above conclusion that RANKL is not related to disease activity, but the effect of the active stage of SLE on bone still needs to be further explored and clarified. Studies on lupus nephritis (LN) showed that OPG levels in active LN were increased, and OPG levels were significantly decreased when disease activity was controlled, suggesting that OPG levels may be increased by inflammatory stimulation, but decreased by GC[ 22 ]. Our study showed that RANKL expression was increased and OPG expression was decreased in the remission state in children with SLE, and respectively they were positively and negatively correlated with duration of SLE and accumulated dose of GC. It speculated that bone remodeling process could keep active by the action of GC. Since the durations of the disease in our patients are all less than 2 years, longer follow-up and observation are needed. One limitation in this part is absence of patients with other rheumatic diseases on corticosteroid therapy, so we will try to collect more patients, which would have been helpful to better clarify whether or not elevated serum RANKL/OPG ratios are primarily related to disease rather than corticosteroid. Another limitation is that we have not done multivariate analysis because of small sample size, and more patients will be enroll our research in the future. At present, the correlation between BMD and RANKL and OPG levels is still inconclusive, but the correlation of increased RANKL/OPG ratio and BMD has been confirmed in many childhood diseases[ 8 , 9 , 10 , 11 ]. Since SLE may lead to decreased bone mineral density, the effect of RANKL and OPG on bone mineral density in SLE active stage is not clear. In our study, all the 40 children with SLE were in remission, so the chronic inflammation in SLE had little effect on RANKL and OPG. Meanwhile, the correlation between GC accumulation and RANKL/OPG suggested that GC played a very important role in regulating the balance of RANKL and OPG. Considering the amount of radiation and the difficulty of operation, whole-body BMD was not performed in these cases, which is a limitation and the correlation between RANKL/OPG and BMD cannot be determined. So we've done animal and cell study to find out the machanisms of the bone loss by GC in SLE model. Vitamin D is an important indicator of bone metabolism, which regulates the balance of calcium and phosphorus. It is also associated with a number of non-skeletal diseases, including cardiovascular disease, cancer, autoimmune disease and diabetes. Serum 25(OH)VitD 3 is considered to be the best indicator of vitamin D status. Low vitamin D level is a risk factor of osteoporosis. Our study also found that children in SLE with have low vitamin D level, which is correlated with accumulated dose of GC, RANKL and OPG. It speculated that GC may participate in bone remodeling process in SLE mediated by the transformation of RANKL/OPG, which characterized by low levels of vitamin D status. But the specific regulatory mechanism still needs further research. Conclusion Children with SLE had low levels of vitamin D3 and bone metabolic abnormalities. In remission of the disease, serum RANKL expression is elevated, OPG expression is reduced, and these changes are associated with duration of SLE and accumulated dose of GC. It states that GC is involved in the process of the occurrence and development of bone metabolic abnormalities through RANKL/OPG which rules out other factors such as disease activity. Abbreviations SLE systemic lupus erythematosus GIOP glucocorticoid-induced osteoporosis GC glucocorticoid OB osteoblastOB OC osteoclast RANKL receptor activator of nuclear factor-κB ligand OPG osteoprotegerin RANK receptor activator of nuclear factor-κB SLICC Systemic Lupus International Collaborating SLEDAI systemic lupus erythematosus disease activity index TNF tumor necrosis factor TRAF6 TNF receptor-related factor TGF transforming growth factor PTH parathormone LN lupus nephritis IL interleukin DXA dual energy x-ray absorptiometry BMD bone mineral density. Declarations Ethical Approval and Consent to participate The studies involving human participants were reviewed and approved by The Ethics Committee of Shanghai Children’s Hospital. Written informed consent to participate in this study was provided by the participants’ legal guardian/next of kin. Consent for publication Each author listed on the manuscript has approved the submission of the manuscript. Availability of supporting data Please contact the corresponding author for data requests. Competing interests The authors declare that they have no competing interests. Funding This work was supported by Natural Science Foundation of Shanghai Children’s Hospital (NO.2020YGZM01 to Sheng Hao). This work was also supported in part by Medical and Industrial cross research Fundation of Shanghai Jiao Tong University (YG2021QN118 to Sheng Hao). Authors' contributions HS and HWY designed the study, drafted the initial manuscript, and reviewed and revised the manuscript. HBX and NXL collected data. ZJ and FD contributed to the detection of RANKL, OPG and VitD3. All authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work. Acknowledgements Natural Science Foundation of Shanghai Children’s Hospital (NO.2020YGZM01). Medical and Industrial cross research Fundation of Shanghai Jiao Tong University (YG2021QN118). Authors' information SH is a Ph.D.-holder and an associate chief physician in a hospital. JZ is a doctoral student in a medical school. BXH an M.A.-holder and a resident doctor in a hospital. DF is an M.A.-holder and a resident doctor in a hospital. XLN is an M.A.-holder and an attending doctor in a hospital. WYH is a Ph.D.-holder and a chief physician and director of the Department of Nephrology and Rheumatology in a hospital. References Bultink IEM. Osteoporosis and fractures in systemic lupus erythematosus. Arthritis Care Res. 2012;64(1):2–8. Petri M, Orbai AM, Graciela S, Alarcón, et al. Derivation and validation of the Systemic Lupus International Collaborating Clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum. 2012;64(8):2677–86. Fanouriakis A, Tziolos N, Bertsias G, et al. Update οn the diagnosis and management of systemic lupus erythematosus. Ann Rheum Dis. 2021;80(1):14–25. Chen X, Wang Z, Duan N, et al. Osteoblast-osteoclast interactions. 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Lupus. 2019;28(10):1233–42. Sandal S, Rawat A, Gupta A, et al. 141 soluble receptor activator of nuclear factor Κ b ligand (s rank-l) levels in paediatric onset sle. Lupus Sci Med. 2017;4(Suppl 1):A64. Gupta R, Aggarwal A, Sinha S, et al. Urinary osteoprotegerin: a potential biomarker of lupus nephritis disease activity. Lupus. 2016;25(11):1230–6. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Major revision 08 May, 2022 Reviews received at journal 14 Mar, 2022 Reviewers invited by journal 10 Mar, 2022 Editor assigned by journal 14 Feb, 2022 Submission checks completed at journal 13 Feb, 2022 Editor invited by journal 13 Feb, 2022 First submitted to journal 05 Feb, 2022 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1331100","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research article","associatedPublications":[],"authors":[{"id":84269471,"identity":"b57b0901-86f8-4d11-b0db-dc60388624c1","order_by":0,"name":"Sheng Hao","email":"","orcid":"","institution":"Shanghai Children's Hospital, Shanghai Jiao Tong University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Sheng","middleName":"","lastName":"Hao","suffix":""},{"id":84269472,"identity":"da8f231a-445d-4648-8f5e-df9cb173b3ff","order_by":1,"name":"Jing Zhang","email":"","orcid":"","institution":"Shanghai Children's Hospital, Shanghai Jiao Tong University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jing","middleName":"","lastName":"Zhang","suffix":""},{"id":84269473,"identity":"aee108e6-305d-41e5-968b-c592e568342a","order_by":2,"name":"Bingxue Huang","email":"","orcid":"","institution":"Shanghai Children's Hospital, Shanghai Jiao Tong University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Bingxue","middleName":"","lastName":"Huang","suffix":""},{"id":84269474,"identity":"82f8b52b-2939-4cff-8478-af2752d83d2b","order_by":3,"name":"Dan Feng","email":"","orcid":"","institution":"Shanghai Children's Hospital, Shanghai Jiao Tong University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Dan","middleName":"","lastName":"Feng","suffix":""},{"id":84269475,"identity":"563f9c22-7e88-4385-b305-9b99d339ddca","order_by":4,"name":"Xiaoling Niu","email":"","orcid":"","institution":"Shanghai Children's Hospital, Shanghai Jiao Tong University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Xiaoling","middleName":"","lastName":"Niu","suffix":""},{"id":84269476,"identity":"f361213e-e65e-488d-a0e1-0761c7df9267","order_by":5,"name":"Wen-Yan Huang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/0lEQVRIiWNgGAWjYBACAyQ24wMGHgxB/FqYDRh4DEjTwiYB5ePXYi6R/Ozh1zabPIPjZ49V/pD5k9jA3rxNgqHmDk4tljPSzI1l29KKDc7kpd3m4TFIbOA5VibBcOwZbofdSDCTltx2OHHDgRyz2wwgLRI5ZhKMDYfxaEn/BtTyP3HD+TdmhT9AWuTfENKSYyb5cduBxA1ABgPYYRI8BLSceVMmzfgvOXHmjTfG0jw8xsZtPGnFFgnH8Gg5nr5N8scZu8S+8zmGH3/2yMn2sx/eeONDDW4tIMAMinOFA0CCsQcYOyChBLwagAp/AAn5BhDzBwGlo2AUjIJRMCIBAOIpV4y45NncAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-2197-0781","institution":"Shanghai Jiaotong University Children's Hospital: Children's Hospital of Shanghai","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Wen-Yan","middleName":"","lastName":"Huang","suffix":""}],"badges":[],"createdAt":"2022-02-06 02:10:13","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1331100/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1331100/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":18379962,"identity":"c78b3d0f-7fc2-4a96-bf8e-054b171a789f","added_by":"auto","created_at":"2022-02-18 20:31:20","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":26094,"visible":true,"origin":"","legend":"\u003cp\u003eSerum RANKL, OPG, and 25(OH) VitD\u003csub\u003e3\u003c/sub\u003e concentrations in children with SLE and healthy children(control group, CTL). \u003cstrong\u003e(A)\u003c/strong\u003e Serum RANKL concentration in SLE group was significantly higher than control group (*\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u0026lt;0.05); \u003cstrong\u003e(B)\u003c/strong\u003e Serum OPG concentration was significantly lower in the SLE group than control group (*\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u0026lt;0.05);\u003cstrong\u003e (C)\u003c/strong\u003e RANKL/OPG ratio in SLE group was significantly higher than control group (*\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u0026lt;0.05)\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-1331100/v1/38e8f70d52d124551af15ce3.png"},{"id":18379963,"identity":"3e8c81c4-9f83-4225-b753-f30f62f7ca21","added_by":"auto","created_at":"2022-02-18 20:31:23","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":409930,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1331100/v1/dbb7751d-d17b-482f-8c5a-f075e710ad67.pdf"}],"financialInterests":"","formattedTitle":"Serum RANKL, OPG and Vitamin D in children with systemic lupus erythematosus","fulltext":[{"header":"Indroduction","content":"\u003cp\u003eSystemic lupus erythematosus (SLE) is an autoimmune multisystem disease with high disability rate and mortality. Glucocorticoid-induced osteoporosis (GIOP) is one of the most common complications of SLE[\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. Bone remodeling is a dynamic equilibrium biological process of the interaction between Osteoblast (OB) and Osteoclast (OC). Receptor activator of nuclear factor-κB ligand (RANKL) and Osteoprotegerin (OPG) act as a pair of biologic active proteins, which bind to their common receptor activator of nuclear factor-κB (RANK) to regulate the balance process of bone remodeling. Glucocorticoid(GC) and inflammation impact bone remodeling by OPG/RANKL/RANK signal system[\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e], which could lead to osteoporosis. In order to define and verify the SLE patients in the presence of RANKL/OPG expression changes and bone metabolic abnormalities, we detected RANKL, OPG and 25(OH)VitD\u003csub\u003e3\u003c/sub\u003e in 40 children with SLE in this study, while correlation between RANKL and other items were analysed, to discuss bone metabolism related changes in SLE condition.\u003c/p\u003e"},{"header":"Participants And Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003ePatients and controls\u003c/h2\u003e \u003cp\u003e 40 children with SLE were enrolled, who were treated and followed up in Shanghai Children's Hospital from January 2019 to July 2020, including 5 males and 35 females. All patients were diagnosed according to 2012 Systemic Lupus International Collaborating(SLICC) classification criteria[\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]. Systemic lupus erythematosus disease activity index(SLEDAI) scores[\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e] of the patients were below 4, which means the disease were in remission. The control group included 40 healthy children who underwent physical examination at the same period, and the age and sex matched. Patients with early onset of lupus-like syndrome due to genetic mutation, SLEDAI scores greater than 4, and severe infection were excluded. The study was approved by the Ethics Committee of Shanghai Children\u0026rsquo;s Hospital(No. 2020R015), and the informed consent was signed by the patient\u0026rsquo;s parents. This study was performed in accordance with the Helsinki Declaration of 1964 and its later amendments.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003eExperimental detection methods\u003c/h2\u003e \u003cp\u003eThe whole blood samples of 40 children with SLE and healthy control group were collected and agglutinated for 30 minutes, then centrifuged at 1000 RPM for 10 min. Serum samples were stored at -80℃. RANKL and OPG were detected by Human Trance/TNFSF11/RANKL ELISA Kit and Human Osteoprotegerin/OPG ELISA Kit(Multisciences Biotech, Hangzhou, China). Serum 25(OH)VitD\u003csub\u003e3\u003c/sub\u003e was detected by Roche cobas E601 Analyzer.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003eStatistical analysis\u003c/h2\u003e \u003cp\u003eSPSS 25.0 statistical software package was used for statistics. Serum RANKL, OPG, 25(OH) VitD\u003csub\u003e3\u003c/sub\u003e concentrations, age, duration of SLE, height, weight and accumulated dose of GC were expressed as mean\u0026thinsp;\u0026plusmn;\u0026thinsp;standard deviation(SD). The comparisons between two groups were used by t-tests, and Pearson analysis was used to analyze the correlation between two indicators in children with SLE.\u003c/p\u003e \u003c/div\u003e"},{"header":"Results","content":"\u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003eGeneral clinical data\u003c/h2\u003e \u003cp\u003eIn this study, there were 40 children in the SLE group and 40 children in the normal control group, and there was no statistical difference in gender composition and age between the two groups (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eGeneral clinical data of children with SLE group and control group (n or mean\u0026thinsp;\u0026plusmn;\u0026thinsp;SD)\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSLE group (n\u0026thinsp;=\u0026thinsp;40)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eControl group (n\u0026thinsp;=\u0026thinsp;40)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003estatistics\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eP value\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eSex(male/female)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e5/35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e6/34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e\u003cspan type=\"BoldItalic\" class=\"BoldItalic\" name=\"Emphasis\"\u003eχ2\u003c/span\u003e\u0026thinsp;=\u0026thinsp;0.3922\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.5312\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAge(years)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e10.75\u0026thinsp;\u0026plusmn;\u0026thinsp;2.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e11.4\u0026thinsp;\u0026plusmn;\u0026thinsp;1.53\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e\u003cspan type=\"BoldItalic\" class=\"BoldItalic\" name=\"Emphasis\"\u003et\u003c/span\u003e\u0026thinsp;=\u0026thinsp;1.378\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.1721\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003eSerum RANKL, OPG and 25(OH) VitD3 concentrations in the SLE group\u003c/h2\u003e \u003cp\u003eSerum RANKL concentration in SLE group was significantly higher than that in normal control group (*P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), and the concentrations of OPG and 25(OH) VitD3 in serum were significantly lower than those in normal control group (*P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), the difference was statistically significant(Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e, Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eSerum concentrations of RANKL, OPG and 25(OH) vitD\u003csub\u003e3\u003c/sub\u003e in SLE and control group (n or mean\u0026thinsp;\u0026plusmn;\u0026thinsp;SD)\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSLE group(n\u0026thinsp;=\u0026thinsp;40)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eControl group (n\u0026thinsp;=\u0026thinsp;40)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eStatistics\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eP value\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eRANKL(pg/ml)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e9.82\u0026thinsp;\u0026plusmn;\u0026thinsp;7.20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e6.80\u0026thinsp;\u0026plusmn;\u0026thinsp;4.35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003et\u0026thinsp;=\u0026thinsp;2.276\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.0256\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eOPG(pg/ml)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e156.34\u0026thinsp;\u0026plusmn;\u0026thinsp;57.33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e189.16\u0026thinsp;\u0026plusmn;\u0026thinsp;68.70\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003et\u0026thinsp;=\u0026thinsp;2.319\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.0230\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e25(OH)vitD\u003csub\u003e3\u003c/sub\u003e(mmol/L)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e43.66\u0026thinsp;\u0026plusmn;\u0026thinsp;31.27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e59.04\u0026thinsp;\u0026plusmn;\u0026thinsp;21.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003et\u0026thinsp;=\u0026thinsp;2.712\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.0082\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003ctfoot\u003e \u003ctr\u003e\u003ctd colspan=\"5\"\u003e*\u003cspan type=\"BoldItalic\" class=\"BoldItalic\" name=\"Emphasis\"\u003eP\u003c/span\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05\u003c/td\u003e\u003c/tr\u003e \u003c/tfoot\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003eCorrelation analysis of serum RANKL level and other indicators in SLE group\u003c/h2\u003e \u003cp\u003eSerum RANKL concentration in children with SLE was correlated with other indexes, including age, course of disease, weight, height, Body mass index (BMI), GC accumulation, OPG and 25(OH) VitD\u003csub\u003e3\u003c/sub\u003e. The results showed that in children with SLE serum RANKL was positively correlated with the duration of SLE and the accumulated dose of GC (*P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), and was negatively correlated with serum OPG and 25(OH) VitD3 (*P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), while height, weight, BMI and age were not correlated (P\u0026thinsp;\u0026gt;\u0026thinsp;0.05). In addition, serum OPG and 25(OH) VitD3 concentrations were inversely correlated with GC cumulants (*P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), the difference was statistically significant(Table\u0026nbsp;\u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab3\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eCorrelation analysis of serum RANKL level and other indexes in children with SLE(n or mean\u0026thinsp;\u0026plusmn;\u0026thinsp;SD\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"4\"\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSLE group(n\u0026thinsp;=\u0026thinsp;40)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eR值\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eP值\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAge(years)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e10.75\u0026thinsp;\u0026plusmn;\u0026thinsp;2.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-0.105\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.519\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eDuration of SLE(Months)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e14.95\u0026thinsp;\u0026plusmn;\u0026thinsp;4.94\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.593\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.0001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eWeight(kg)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e42.33\u0026thinsp;\u0026plusmn;\u0026thinsp;9.68\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-0.279\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.081\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eHeight(cm)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e131.33\u0026thinsp;\u0026plusmn;\u0026thinsp;13.92\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-0.141\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.385\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eBMI(kg/m\u003csup\u003e2\u003c/sup\u003e)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e24.64\u0026thinsp;\u0026plusmn;\u0026thinsp;4.88\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-0.176\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.278\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAccumulated dose of GC (mg/kg)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e292.33\u0026thinsp;\u0026plusmn;\u0026thinsp;67.93\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.727\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.0001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eRANKL(pg/ml)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e9.82\u0026thinsp;\u0026plusmn;\u0026thinsp;7.20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e-\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eOPG(pg/ml)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e156.34\u0026thinsp;\u0026plusmn;\u0026thinsp;57.33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-0.601/-0.66\u003csup\u003e**\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;0.0001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e25(OH)vitD\u003csub\u003e3\u003c/sub\u003e(mmol/L)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e43.66\u0026thinsp;\u0026plusmn;\u0026thinsp;31.27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e-0.469/-0.508\u003csup\u003e**\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.002\u003csup\u003e*\u003c/sup\u003e/-0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003ctfoot\u003e \u003ctr\u003e\u003ctd colspan=\"4\"\u003e*P\u0026thinsp;\u0026lt;\u0026thinsp;0.05, the difference was statistically significant\u003c/td\u003e\u003c/tr\u003e \u003ctr\u003e\u003ctd colspan=\"4\"\u003e** The correlation with accumulated dose of GC\u003c/td\u003e\u003c/tr\u003e \u003c/tfoot\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e"},{"header":"Discussion","content":"\u003cp\u003eBone is a constantly metabolizing organ, and bone remodeling is a dynamic balance process of bone resorption and bone formation. OC absorbs old bone and Osteoblast OB forms new bone, respectively. Bone remodeling relies on the precise coordination of the two kinds of cells to keep in balance. OC can regulate the functions of OB in both positive and negative ways, while OB can adjust OC through OPG/RANKL/RANK signaling system. RANKL and OPG are a pair of important bioactive proteins in regulating the balance of bone metabolism, which are widely expressed in lymphoid tissues. RANKL is a member of tumor necrosis factor(TNF) superfamily, which activates downstream related molecules after binding to its receptor RANK to enhance osteoclast differentiation and activity, then leads to increased bone resorption. RANKL is highly expressed in the thymus gland, lungs and lymph nodes, and low expressed in the spleen and bone marrow[\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. A variety of cytokines, hormones, growth factors can regulate the expression of RANKL. RANK and OPG are also members of TNF Receptor superfamily. RANKL binds to RANK on the surface of osteoclasts to induce the accumulation of TNF receptor-related factor (TRAF6) in osteoclasts and promotes the differentiation and activation of osteoclasts, while the apoptosis of osteoclasts was inhibited. OPG has two forms including monomer and dimer, and is mainly expressed in osteoblasts and vascular cells[\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. It can competitively bind RANK, so the ratio of RANKL/OPG plays a key role in the process of bone remodeling. Our study mainly detected serum RANKL and OPG in children with SLE.\u003c/p\u003e \u003cp\u003eThe expression of RANKL, OPG and RANK is regulated by different hormones and cytokines, including Parathormone(PTH), 1,25(OH)\u003csub\u003e2\u003c/sub\u003eVitD\u003csub\u003e3\u003c/sub\u003e, prostaglandins, TNF-α, Interleukin (IL)-6 and M-CSF, etc. The high expression of estrogen could increase the expressions of transforming growth factor (TGF) -β and OPG, which inhibited RANKL signal transduction, and then promoted bone formation. On the contrary, the low expression of estrogen could significantly increase the expressions of IL-1, IL-6, TNF-α and other pro-inflammatory factors, which increased the expression of RANKL and promoted bone resorption[\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. At present, the most commonly used method for bone mineral density(BMD) assessment is dual energy x-ray absorptiometry (DXA). But local DXA is insufficient to reflect the condition, and the whole body DXA is difficult to perform in children because of the high dose of radiation and coordination problem. PTH, 25 (OH)VitD\u003csub\u003e3\u003c/sub\u003e and osteocalcin and can only represent the body endocrine levels, so that new and simple biomarkers are needed, which are the reflection of bone metabolic state. Fracture risk assessment should be performed on all patients with long-term oral GC and should also be regularly assessed during treatment[\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. RANKL and OPG play a key role in the balance of osteoblasts and osteoclasts, which are very important in the regulation of bone metabolism in vivo and believed to be related to the reduction of bone mineral density in patients with SLE. A number of studies have shown that the RANKL/OPG ratio is decreased in chronic diseases such as type 1 diabetes, juvenile idiopathic arthritis, and nephrotic syndrome[\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e, \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e, \u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e, \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e], which may represent the negative balance of bone remodeling. Therefore, the detection of RANKL and OPG may become new sensitive biomarkers to evaluate bone metabolism in children.\u003c/p\u003e \u003cp\u003eIt is reported that annual incidence of SLE in adults ranges from 0.3 to 31.5/100,000[\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e], and 30 to 70/100,000 in China[\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e]. The incidence in children ranges from 0.36 to 2.50/ 100,000, and the prevalence ranges from 1.89 to 25.70/100,000[\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. Although the incidence of SLE in children is not high, the vast majority of children with SLE need lifelong treatment, and the mortality is still high in young patients[\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e]. GC is widely used in patients with inflammatory, autoimmune and allergic diseases, and is one of the most common and irreplaceable drugs for SLE at present. With the application of GC and other immunosuppressants, the mortality of SLE patients has decreased significantly in recent decades. More than 80% of patients with SLE need long-term duration[\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e] so that GIOP is more common. GC stimulates RANKL and inhibit OPG, which promotes osteoclast differentiation and osteolysis, while it induces osteocyte apoptosis and inhibits osteocyte generation[\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. Studies have shown that bone loss caused by the GC can be roughly divided into two stages, bone mineral density loss fast which is about 6\u0026thinsp;~\u0026thinsp;12% in the first year of treatment, and about 3% a year later[\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. At the later stages, GC increased apoptosis of osteoblasts and osteocytes, and then the expression of RANKL decreased[\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e], while the number of osteoclasts decreased by apoptosis and autophagy. In addition, control of inflammation and reduction of GC dosage at the later stage also resulted in less bone loss. A study found that OPG, RANKL and RANKL/OPG were significantly increased in the SLE group, and OPG level was related to the activity of the disease[\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]. Studies on children with SLE also showed that RANKL level was not related to disease activity[\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e]. Therefore our study selected the patients with disease activity controlled to reduce the interference of inflammation. The results showed that serum RANKL and OPG concentrations in inactive state of disease are also significantly different with normal children. It suggested that dynamic balance regulation of bone metabolism still exists in the remission state of the disease, partially verifying the above conclusion that RANKL is not related to disease activity, but the effect of the active stage of SLE on bone still needs to be further explored and clarified. Studies on lupus nephritis (LN) showed that OPG levels in active LN were increased, and OPG levels were significantly decreased when disease activity was controlled, suggesting that OPG levels may be increased by inflammatory stimulation, but decreased by GC[\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e]. Our study showed that RANKL expression was increased and OPG expression was decreased in the remission state in children with SLE, and respectively they were positively and negatively correlated with duration of SLE and accumulated dose of GC. It speculated that bone remodeling process could keep active by the action of GC. Since the durations of the disease in our patients are all less than 2 years, longer follow-up and observation are needed. One limitation in this part is absence of patients with other rheumatic diseases on corticosteroid therapy, so we will try to collect more patients, which would have been helpful to better clarify whether or not elevated serum RANKL/OPG ratios are primarily related to disease rather than corticosteroid. Another limitation is that we have not done multivariate analysis because of small sample size, and more patients will be enroll our research in the future.\u003c/p\u003e \u003cp\u003eAt present, the correlation between BMD and RANKL and OPG levels is still inconclusive, but the correlation of increased RANKL/OPG ratio and BMD has been confirmed in many childhood diseases[\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e, \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e, \u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e, \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. Since SLE may lead to decreased bone mineral density, the effect of RANKL and OPG on bone mineral density in SLE active stage is not clear. In our study, all the 40 children with SLE were in remission, so the chronic inflammation in SLE had little effect on RANKL and OPG. Meanwhile, the correlation between GC accumulation and RANKL/OPG suggested that GC played a very important role in regulating the balance of RANKL and OPG. Considering the amount of radiation and the difficulty of operation, whole-body BMD was not performed in these cases, which is a limitation and the correlation between RANKL/OPG and BMD cannot be determined. So we've done animal and cell study to find out the machanisms of the bone loss by GC in SLE model.\u003c/p\u003e \u003cp\u003eVitamin D is an important indicator of bone metabolism, which regulates the balance of calcium and phosphorus. It is also associated with a number of non-skeletal diseases, including cardiovascular disease, cancer, autoimmune disease and diabetes. Serum 25(OH)VitD\u003csub\u003e3\u003c/sub\u003e is considered to be the best indicator of vitamin D status. Low vitamin D level is a risk factor of osteoporosis. Our study also found that children in SLE with have low vitamin D level, which is correlated with accumulated dose of GC, RANKL and OPG. It speculated that GC may participate in bone remodeling process in SLE mediated by the transformation of RANKL/OPG, which characterized by low levels of vitamin D status. But the specific regulatory mechanism still needs further research.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eChildren with SLE had low levels of vitamin D3 and bone metabolic abnormalities. In remission of the disease, serum RANKL expression is elevated, OPG expression is reduced, and these changes are associated with duration of SLE and accumulated dose of GC. It states that GC is involved in the process of the occurrence and development of bone metabolic abnormalities through RANKL/OPG which rules out other factors such as disease activity.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cdiv class=\"DefinitionList\"\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eSLE\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003esystemic lupus erythematosus\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eGIOP\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eglucocorticoid-induced osteoporosis\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eGC\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eglucocorticoid\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eOB\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eosteoblastOB\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eOC\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eosteoclast\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eRANKL\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003ereceptor activator of nuclear factor-κB ligand\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eOPG\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eosteoprotegerin\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eRANK\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003ereceptor activator of nuclear factor-κB\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eSLICC\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eSystemic Lupus International Collaborating\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eSLEDAI\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003esystemic lupus erythematosus disease activity index\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eTNF\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003etumor necrosis factor\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eTRAF6\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eTNF receptor-related factor\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eTGF\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003etransforming growth factor\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003ePTH\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003eparathormone\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eLN\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003elupus nephritis\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eIL\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003einterleukin\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eDXA\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003edual energy x-ray absorptiometry\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv class=\"DefinitionListEntry\"\u003e \u003cdiv class=\"Term\"\u003eBMD\u003c/div\u003e \u003cdiv class=\"Description\"\u003e \u003cp\u003ebone mineral density.\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003c/div\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthical Approval and Consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe studies involving human participants were reviewed and approved by The Ethics Committee of Shanghai Children\u0026rsquo;s Hospital. Written informed consent to participate in this study was provided by the participants\u0026rsquo; legal guardian/next of kin.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eEach author listed on the manuscript has approved the submission of the manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of supporting data\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePlease contact the corresponding author for data requests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no competing interests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis work was supported by Natural Science Foundation of Shanghai Children\u0026rsquo;s Hospital (NO.2020YGZM01 to Sheng Hao). This work was also supported in part by Medical and Industrial cross research Fundation of Shanghai Jiao Tong University (YG2021QN118 to Sheng Hao).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026apos; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eHS and HWY designed the study, drafted the initial manuscript, and reviewed and revised the manuscript. HBX and NXL collected data. ZJ and FD contributed to the detection of RANKL, OPG and VitD3. All authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNatural Science Foundation of Shanghai Children\u0026rsquo;s Hospital (NO.2020YGZM01).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMedical and Industrial cross research Fundation of Shanghai Jiao Tong University (YG2021QN118).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026apos; information\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSH is a Ph.D.-holder and an associate chief physician in a hospital.\u003c/p\u003e\n\u003cp\u003eJZ is a doctoral student in a medical school.\u003c/p\u003e\n\u003cp\u003eBXH an M.A.-holder and a resident doctor in a hospital.\u003c/p\u003e\n\u003cp\u003eDF is an M.A.-holder and a resident doctor in a hospital.\u003c/p\u003e\n\u003cp\u003eXLN is an M.A.-holder and an attending doctor in a hospital.\u003c/p\u003e\n\u003cp\u003eWYH is a Ph.D.-holder and a chief physician and director of the Department of Nephrology and Rheumatology in a hospital.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eBultink IEM. Osteoporosis and fractures in systemic lupus erythematosus. Arthritis Care Res. 2012;64(1):2\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePetri M, Orbai AM, Graciela S, Alarc\u0026oacute;n, et al. Derivation and validation of the Systemic Lupus International Collaborating Clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum. 2012;64(8):2677\u0026ndash;86.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eFanouriakis A, Tziolos N, Bertsias G, et al. Update οn the diagnosis and management of systemic lupus erythematosus. Ann Rheum Dis. 2021;80(1):14\u0026ndash;25.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChen X, Wang Z, Duan N, et al. Osteoblast-osteoclast interactions. Connect Tissue Res. 2018;59(2):99\u0026ndash;107.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOmar HS, Shaker OG, Nassar YH, et al. The association between RANKL and Osteoprotegerin gene polymorphisms with breast cancer. Mol Cell Biochem. 2015;403(1\u0026ndash;2):219\u0026ndash;29.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNagy V, Penninger JM. The RANKL-RANK Story. Gerontology. 2015;61(6):534\u0026ndash;42.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChotiyarnwong P, McCloskey EV. Pathogenesis of glucocorticoid-induced osteoporosis and options for treatment. Nat Rev Endocrinol. 2020;16(8):437\u0026ndash;47.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTsentidis C, Gourgiotis D, Kossiva L, et al. Higher levels of s-RANKL and osteoprotegerin in children and adolescents with type 1 diabetes mellitus may indicate increased osteoclast signaling and predisposition to lower bone mass: a multivariate cross-sectional analysis. Osteoporos Int. 2016;27(4):1631\u0026ndash;43.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLien G, Ueland T, Godang K, et al. Serum levels of osteoprotegerin and receptor activator of nuclear factor -κB ligand in children with early juvenile idiopathic arthritis: a 2-year prospective controlled study. Pediatr Rheumatol Online J. 2010;8(1):30.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOzkaya O, Buyan N, Bideci A, et al. Osteoprotegerin and RANKL serum levels and their relationship with serum ghrelin in children with chronic renal failure and on dialysis. Nephron Clin Pract. 2007;105(4):c153\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWasilewska A, Rybi-Szuminska A, Zoch-Zwierz W. Serum RANKL. osteoprotegerin (OPG), and RANKL/OPG ratio in nephrotic children. Pediatr Nephrol. 2010;25(10):2067\u0026ndash;75.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChinese Rheumatology Association, National Clinical Research Center for Dermatologic and Immunologic Diseases, \u0026amp; Chinese Systemic Lupus Erythematosus Treatment and Research Group. 2020 Chinese guidelines for the diagnosis and treatment of systemic lupus erythematosus. Zhonghua nei ke za zhi. 2020;59(3):172\u0026ndash;85.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePineles D, Valente A, Warren B, et al. Worldwide incidence and prevalence of pediatric onset systemic lupus erythematosus. Lupus. 2011;20(11):1187\u0026ndash;92.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNieves CEF, Izmirly PM. Mortality in Systemic Lupus Erythematosus: an Updated Review. Curr Rheumatol Rep. 2016;18(4):21.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRuiz-Arruza I, Ugarte A, Cabezas-Rodriguez I, et al. Glucocorticoids and irreversible damage in patients with systemic lupus erythematosus. Rheumatology (Oxford). 2014;53(8):1470\u0026ndash;6.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eApostolopoulos D, Kandane-Rathnayake R, Raghunath S, et al. Independent association of glucocorticoids with damage accrual in SLE. Lupus Sci Med. 2016;3(1):e000157.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eBultink IE, Baden M, Lems WF. Glucocorticoid-induced osteoporosis: an update on current pharmacotherapy and future directions. Expert Opin Pharmacother. 2013;14(2):185\u0026ndash;97.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOshagbemi OA, Burden AM, Shudofsky KN, et al. Use of high-dose intermittent systemic glucocorticoids and the risk of fracture in patients with chronic obstructive pulmonary disease. Bone. 2018;110:238\u0026ndash;43.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTeitelbaum. Bone: the conundrum of glucocorticoid-induced osteoporosis. Nat Rev Endocrinol. 2012;8(8):451\u0026ndash;2.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAli R, Hammad A, El-Nahrery E, et al. Serum RANKL, osteoprotegerin (OPG) and RANKL/OPG ratio in children with systemic lupus erythematosus. Lupus. 2019;28(10):1233\u0026ndash;42.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSandal S, Rawat A, Gupta A, et al. 141 soluble receptor activator of nuclear factor Κ b ligand (s rank-l) levels in paediatric onset sle. Lupus Sci Med. 2017;4(Suppl 1):A64.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGupta R, Aggarwal A, Sinha S, et al. Urinary osteoprotegerin: a potential biomarker of lupus nephritis disease activity. Lupus. 2016;25(11):1230\u0026ndash;6.\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"pediatric-rheumatology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"proj","sideBox":"Learn more about [Pediatric Rheumatology](http://ped-rheum.biomedcentral.com)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/proj/default.aspx","title":"Pediatric Rheumatology","twitterHandle":"@BioMedCentral","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Pediatric systemic lupus erythematosus, Receptor activator of nuclear factor-κB ligand, Osteoprotegerin, Vitamin D, Glucocorticoid","lastPublishedDoi":"10.21203/rs.3.rs-1331100/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1331100/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eIndroduction: \u003c/strong\u003eSLE is an autoimmune multisystem disease. Glucocorticoid is an irreplaceable medication for SLE. Glucocorticoid and inflammation impact bone remodeling by OPG/RANKL/RANK signal system, which could lead to osteoporosis. Our aim is to clarify the expression of RANKL/OPG in children with SLE, and to preliminarily explore the changes of bone metabolism related indexes in children with SLE. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eMethods: \u003c/strong\u003eSerum RANKL and OPG of 40 children with SLE and healthy children were detected by ELISA, while 25(OH)VitD\u003csub\u003e3\u003c/sub\u003e was detected routinely. Clinical data of children with SLE were recorded, including gender, age, height, weight, BMI, duration of the disease, accumulated dose of glucocorticoid, and correlation analysis was conducted with RANKL, OPG and 25(OH)VitD\u003csub\u003e3\u003c/sub\u003e. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eResults: \u003c/strong\u003eSerum RANKL concentration in SLE group was significantly higher than health group (9.82±7.20 vs. 6.80±4.35 pg/ml, P\u0026lt;0.05), and the concentrations of OPG and 25(OH)VitD\u003csub\u003e3\u003c/sub\u003e in serum were significantly lower than health group (156.34±57.33 vs. 189.16±68.70 pg/ml and 43.66±31.27 vs. 59.04±21.56 mmol/L, P\u0026lt;0.05). Serum RANKL in children with SLE was positively correlated with the duration of SLE, accumulated dose of GC(r=0.593, 0.727, P\u0026lt;0.05). And it was negatively correlated with serum OPG and 25(OH)VitD\u003csub\u003e3\u003c/sub\u003e (r=-0.601, -0.469, P\u0026lt;0.05). In addition, serum OPG and 25(OH)VitD\u003csub\u003e3 \u003c/sub\u003econcentrations were inversely correlated with accumulated dose of GC (r=-0.66, -0.508, P\u0026lt;0.05). \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusion: \u003c/strong\u003eLow levels of vitamin D3 and bone metabolic abnormalities were found in children with SLE under the condition of disease remission, while serum RANKL expression was elevated, OPG expression was reduced. These changes associated with the duration of SLE, accumulated dose of GC. In the case of exclusion of disease activity, GC and other factors may be involved in the occurrence and development of abnormal bone metabolism through RANKL/OPG.\u003c/p\u003e","manuscriptTitle":"Serum RANKL, OPG and Vitamin D in children with systemic lupus erythematosus","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-02-18 20:31:19","doi":"10.21203/rs.3.rs-1331100/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revision","date":"2022-05-08T21:41:41+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2022-03-14T04:19:40+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2022-03-11T03:21:35+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2022-02-14T09:38:19+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2022-02-13T23:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2022-02-13T23:00:00+00:00","index":"","fulltext":""},{"type":"submitted","content":"Pediatric Rheumatology","date":"2022-02-05T21:09:46+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"pediatric-rheumatology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"proj","sideBox":"Learn more about [Pediatric Rheumatology](http://ped-rheum.biomedcentral.com)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/proj/default.aspx","title":"Pediatric Rheumatology","twitterHandle":"@BioMedCentral","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"cb4e353b-51aa-4a95-aaac-0add2ffc7b61","owner":[],"postedDate":"February 18th, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2022-07-19T02:50:06+00:00","versionOfRecord":[],"versionCreatedAt":"2022-02-18 20:31:19","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1331100","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1331100","identity":"rs-1331100","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}