Design and Development of Advanced Biocompatible Hydrogels for Burn Wound Healing Applications

Design and Development of Advanced Biocompatible Hydrogels for Burn Wound Healing Applications | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Design and Development of Advanced Biocompatible Hydrogels for Burn Wound Healing Applications Manju Paramshetty, Zainab Khan, Shashank Lokesh, Chaitanya Parmar, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7439402/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 12 You are reading this latest preprint version Abstract Burn wound healing poses a significant medical challenge and remains a critical global health concern, necessitating innovative approaches to improve treatment outcomes. The rising prevalence of burn injuries emphasizes the pressing need for advanced wound dressings capable of providing antimicrobial protection, maintaining a moist healing environment, and effectively managing exudative fluids. Hydrogels are particularly notable for their biocompatibility, sustainability, and multifunctionality and have emerged as promising materials to meet these requirements. This study focuses on the development of novel hydrogels using acetic acid (AA) and gelatin as base materials, supplemented with functional additives such as chitosan (CS), selenium (SE), Ag (Ag) nanopowders, Cu (Cu) nanopowders, and natural bioactive compounds including almond oil, neem oil, propolis, and vitamins A and C. The study involves hydrogel preparation, comprehensive characterization, and functional performance evaluation. The hydrogels were characterized using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and X-ray Diffraction (XRD) employed to investigate the chemical, thermal, and crystalline properties. Swelling behavior and drug release kinetics were analyzed to assess their moisture retention capacity and healing agent release capabilities, while biocompatibility was assessed using methyl thiazolyl tetrazolium (MTT) assays to determine cytotoxicity and cell viability at varying concentrations. The results demonstrated that SE-AA-based hydrogels exhibited superior cell viability and biocompatibility, effectively supporting tissue regeneration, cellular attachment, and growth. This study highlights the potential of multifunctional hydrogels as advanced wound dressings, offering a significant advancement in burn wound healing applications. These findings pave the way for the development of next-generation hydrogels tailored to enhance clinical outcomes and improve the quality of life for burn patients. Multifunctional hydrogels burn wound healing biocompatibility cytotoxicity drug release Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 09 Oct, 2025 Reviews received at journal 23 Sep, 2025 Reviews received at journal 22 Sep, 2025 Reviews received at journal 14 Sep, 2025 Reviewers agreed at journal 28 Aug, 2025 Reviewers agreed at journal 27 Aug, 2025 Reviewers agreed at journal 27 Aug, 2025 Reviewers agreed at journal 27 Aug, 2025 Reviewers invited by journal 27 Aug, 2025 Editor assigned by journal 26 Aug, 2025 Submission checks completed at journal 26 Aug, 2025 First submitted to journal 23 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7439402","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":507128379,"identity":"e2c1f53c-c25b-4ef0-9b46-ffa2267aa284","order_by":0,"name":"Manju Paramshetty","email":"","orcid":"","institution":"Wichita State University","correspondingAuthor":false,"prefix":"","firstName":"Manju","middleName":"","lastName":"Paramshetty","suffix":""},{"id":507128380,"identity":"c98d0d40-8886-4875-81e5-148b5aa2a637","order_by":1,"name":"Zainab Khan","email":"","orcid":"","institution":"Wichita State 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