Effect of butyrate on glucose uptake and de novo lipogenesis in adipocytes.

(A) Glucose uptake assay in adipocytes. (B) De novo lipogenesis assay in adipocytes. Cells were treated with butyrate at 1500 μM concentration throughout the differentiation period (day 0 to 8). On day 8, cells were incubated overnight with DMEM/F12 containing 0.1% BSA in the absence of insulin. On day 9, cells were subjected to either glucose uptake or de novo lipogenesis assay in the absence or presence of insulin. Data were analyzed by two-way ANOVA with Tukey multiple comparison test. Data are means ± SE (n = 20). Different letters on bars indicate significant differences (P<0.05).