Machine Learning Discoveries of AURKB-X Synergy in ETC-1922159 Treated Colorectal Cancer Cells

Aurora kinase B (AURKB) is one of the components that make up a complex called the chromosome passenger complex (CPC). The major functions of AURKB are to regulate kinetochore-microtubule attachments as well as cytokinesis. In colorectal cancer (CRC) cells treated with ETC-1922159, AURKB was found to be down regulated along with other genes. A recently developed search engine ranked combinations of AURKB-X (X, a particular gene/protein) at 2nd order level after drug administration. Some of these combinations have been tested in wet lab, however many have been pointed out by the search engine that are yet to be explored/tested. These rankings re- veal which AURKB-X combinations might be working synergistically in CRC. In this research work, I cover combinations of AURKB with ZW10 interacting kinetochore protein (ZWINT/ZWINT-1), inner centromere protein (INCENP), targeting protein for xenopus kinesin-like protein 2 (TPX2), WNT pathway components, chromatin licens- ing and DNA replication factor 1 (CDT1), G2 and S-phase expressed 1 (GTSE1), vaccinia related kinase 1 (VRK1), RecQ like helicase 4 (RECQL4), histone H3 associated protein kinase (HASPIN/GSG2), centromere protein (CENP), E2F transcription factor (E2F), cell division cycle (CDC), ubiquitin specific peptidase (USP), Nucleoporin (NUP), gem nuclear organelle associated protein (GEMIN), mitotic arrest deficient 2 like (MAD2L), homeobox (HOX), DExH-box helicase (DHX), polo like kinase (PLK), budding uninhibited by benzimidazoles (BUB), DNA topoisomerase (TOP), cyclin dependent kinase (CDK), alkB homolog lysine demethylase (ALKBH), protein arginine methyltransferase (PRMT), shugoshin (SGO), spindle and kinetochore associated com- plex subunit (SKA), growth arrest specific (GAS) and kinesin family member (KIF) family.