Microscopic and Spectroscopic insights of antibacterial mechanism of Ag-loaded TiO 2 Electrospun nanofibers

Microscopic and Spectroscopic insights of antibacterial mechanism of Ag-loaded TiO 2 Electrospun nanofibers | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Microscopic and Spectroscopic insights of antibacterial mechanism of Ag-loaded TiO 2 Electrospun nanofibers Bisma Khanam, Shahzad Anwar, Muhammad Zakria, Taj Muhammad Khan, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4210161/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Pristine TiO2 nanofibers with a range of Ag-loading (wt.% 0, 2, 4, 6 and 8) were prepared by electrospinning technique whose mechanistic insight into their improved antibacterial activity against E.coli as a model microorganism has been investigated. Calcination of as prepared electrospun nanofibers was carried out in ambient air at 500℃ for 3h to promote transformation crystalline phase of TiO2 from anatase to the rutile, removal of reaction moieties and genesis of Ag clusters in these nanofibers. The physicochemical properties of nanofiber samples were analyzed and observed by employing Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and Raman spectroscopy. On the whole, these nanofibers have been found to exhibit a smooth surface morphology with minimal beading effect and sustained compositions of the original elements. Heat treatment of the as prepared samples lead to preferential formation of rutile phase as a function of wt.% loading of Ag that strongly supported phase transformation of TiO2 from anatase to rutile. These nanofibers have been tested for their antimicrobial efficacy against the E. Coli bacterium by Well plate method. The results have exhibited formation of consistent zones of inhibition and log10 CFU/mL with the rising content of Ag loading in the TiO2 matrix, while highest antimicrobial efficacy has been observed with the sample containing 8wt.% of Ag loading. Confocal microscopy and Scanning electron microscopy analysis of treated bacterial samples has disclosed the interaction between nanofibers and bacterial cells, resulting into disruption of cell membrane. This disruption led to the leakage of cell contents and ultimately causing bacterial cell death. Electrospun Nanofibers FESEM Confocal microscopy fluorescence spectroscopy Antibacterial efficacy Reactive oxygen species (ROS) Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryFile2Aprail2024.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4210161","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":288743871,"identity":"354d337f-30e7-4eda-bcc5-bd0bf8915fce","order_by":0,"name":"Bisma Khanam","email":"","orcid":"","institution":"Government College University (GCU)","correspondingAuthor":false,"prefix":"","firstName":"Bisma","middleName":"","lastName":"Khanam","suffix":""},{"id":288743872,"identity":"7acf6aac-61fc-4ebc-885c-e9b80b791b42","order_by":1,"name":"Shahzad Anwar","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABDUlEQVRIiWNgGAWjYBAC+RkMbECKGcJLMLCRY2Dgwa/F4AaylgcFacaEtUggaWF88OFwYgNBLdLNzx7+qLGWN5dIfvYhwYA5fcPxswcffGCwk9NtwOGXOcfMDSSOpRvunJFmPCPBgC13w5m8ZMMZDMnGZgdwWHMjwUzCgO0w44bbCcZA7/PkbjiQYybNw3AgcRtOLenfJBL+HbbfcDv9M1CLRLrB+TeEtOSYSRxsO5y44XYOyBaDBIMbBGwBKiiTbOxLT95w/00xUEuC4cwbb4wNZxjg9ov8jPRtkj++WdtuOHN8M+OPP//l+c7nGD74UGEnh9P7GEABrNKAWOVgextIUT0KRsEoGAUjAQAA+4VjnEiLoukAAAAASUVORK5CYII=","orcid":"","institution":"National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, Islamabad 45650, Pakistan","correspondingAuthor":true,"prefix":"","firstName":"Shahzad","middleName":"","lastName":"Anwar","suffix":""},{"id":288743873,"identity":"3fcd15fe-d42f-4b29-9e75-1d7a6ecaa2c4","order_by":2,"name":"Muhammad Zakria","email":"","orcid":"","institution":"National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, Islamabad 45650, Pakistan","correspondingAuthor":false,"prefix":"","firstName":"Muhammad","middleName":"","lastName":"Zakria","suffix":""},{"id":288743874,"identity":"d4f25404-4202-4a9b-a4fa-2c1352ee0c7a","order_by":3,"name":"Taj Muhammad Khan","email":"","orcid":"","institution":"National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, Islamabad 45650, Pakistan","correspondingAuthor":false,"prefix":"","firstName":"Taj","middleName":"Muhammad","lastName":"Khan","suffix":""},{"id":288743875,"identity":"6e960305-1d3e-43c0-beff-c8a4e57d791a","order_by":4,"name":"A. 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