Lacticaseibacillus paracasei-Derived Cell-Free Supernatant-10 as a Novel Therapeutic for Acne Vulgaris: Efficacy and Mechanisms in a Murine Model

Lacticaseibacillus paracasei-Derived Cell-Free Supernatant-10 as a Novel Therapeutic for Acne Vulgaris: Efficacy and Mechanisms in a Murine Model | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 7 January 2026 V1 Latest version Share on Lacticaseibacillus paracasei-Derived Cell-Free Supernatant-10 as a Novel Therapeutic for Acne Vulgaris: Efficacy and Mechanisms in a Murine Model Authors : Xu Zhou , Jiahui Xu , Xiaoyong Chen , Qin Qin , Zsolt Zalán , and Huayi Suo [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.176778924.44802054/v1 139 views 76 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Previous research has shown that the cell-free supernatant (CFS) from Lacticaseibacillus paracasei has potent antimicrobial properties, but the mechanisms underlying its therapeutic effects on acne vulgaris remain unclear. We investigated the effects of L. paracasei LPH01 cell-free supernatant (CFS-10) using a Propionibacterium acnes -induced acne-like mouse model. Our results demonstrated that topical CFS-10 treatment significantly alleviated skin lesions, reduced inflammatory cell infiltration, and suppressed pro-inflammatory cytokine expression in acne-induced mice. Furthermore, CFS-10 substantially inhibited skin lipid synthesis, and reduced sebum secretion by downregulating the expression of key genes and proteins involved in the PPAR-γ/SREBP-1 signaling pathway. Transcriptome analysis revealed that CFS-10 mitigated inflammation by inhibiting the TLR4/NF-κB signaling pathway and reducing inflammation-related gene expression. Additionally, CFS-10 strengthened the skin barrier, restored skin microbiota homeostasis, increased beneficial Lactobacillus species, and decreased pathogenic Staphylococcus . Our results provide thereby establishing a robust theoretical foundation for its therapeutic application in acne management. Supplementary Material File (figures.docx) Download 43.09 MB File (manuscript.docx) Download 83.95 KB Information & Authors Information Version history V1 Version 1 07 January 2026 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords atopic dermatitis dermatology inflammation microbiome Authors Affiliations Xu Zhou Southwest University College of Food Science View all articles by this author Jiahui Xu Southwest University College of Food Science View all articles by this author Xiaoyong Chen Southwest University College of Food Science View all articles by this author Qin Qin The First Affiliated Hospital of Chongqing Medical University View all articles by this author Zsolt Zalán Hungarian University of Agriculture and Life Sciences View all articles by this author Huayi Suo [email protected] Southwest University College of Food Science View all articles by this author Metrics & Citations Metrics Article Usage 139 views 76 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Xu Zhou, Jiahui Xu, Xiaoyong Chen, et al. Lacticaseibacillus paracasei-Derived Cell-Free Supernatant-10 as a Novel Therapeutic for Acne Vulgaris: Efficacy and Mechanisms in a Murine Model. Authorea . 07 January 2026. DOI: https://doi.org/10.22541/au.176778924.44802054/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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